FastQCFastQC Report
Wed 25 May 2016
SRR1779697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT1296.574923547400611No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT462.344546381243629RNA PCR Primer, Index 24 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC321.6309887869520898RNA PCR Primer, Index 24 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC150.764525993883792No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC130.6625891946992865No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG90.45871559633027525No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC80.40774719673802245No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC70.3567787971457696No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT60.3058103975535168No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT60.3058103975535168RNA PCR Primer, Index 15 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG60.3058103975535168No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT50.254841997961264Illumina PCR Primer Index 10 (95% over 23bp)
ACCTAAAACAATTTCACAGCACCAAATCTCCACCTCCATCATCACCTCAA50.254841997961264No Hit
CAGACCCAGCCGAGGACGCGACCCAGACCCACGCTGAGCTGCGCGCCCGC50.254841997961264No Hit
GCTGCAGGCCGAGTCCGGCCGGGGGCAGGAGCAGTCCTGTCTCTTATACA50.254841997961264No Hit
TCCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCC40.20387359836901123No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT40.20387359836901123TruSeq Adapter, Index 5 (96% over 26bp)
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA40.20387359836901123No Hit
ATGTACGGTAAATGGCTTTATGTACTATGTACTGTTAAGGGTGGGTAGGT40.20387359836901123No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT40.20387359836901123No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT40.20387359836901123RNA PCR Primer, Index 24 (95% over 22bp)
GGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATT40.20387359836901123No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT40.20387359836901123RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG30.1529051987767584No Hit
GTGCGGGCGCACGGACAGCGGCAGGTCCAGGTAGGAACCGGTCTTGAGGA30.1529051987767584No Hit
ACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGGCCATAAT30.1529051987767584No Hit
AGATGAGAGAGCAGCAGGGAACGCTGCTCTGAGGGACGGGGCGACGGGGA30.1529051987767584No Hit
CTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCAT30.1529051987767584No Hit
TATCACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT30.1529051987767584RNA PCR Primer, Index 24 (95% over 23bp)
GATAGTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAG30.1529051987767584No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC30.1529051987767584No Hit
AGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTGGGACTGTCTACTGAG30.1529051987767584No Hit
GTCGGTGAACGCGCCGGCCATGTGTGGCACTGCTCGCTGTCGTTGCGTGC30.1529051987767584No Hit
TGTGGGTGCTGCTCGGCCACGAGCCCTCCGTGGTCCGCGCCGGCTGGCCC30.1529051987767584No Hit
GTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTC30.1529051987767584No Hit
GATTATGGGCGTTGATTAGTAGTAGTTACTGGTTGAACATTGTTTGTTGG30.1529051987767584No Hit
TCAGAGAGCTTGTCGTTCCAGTGAGCTGCGGCGACCTCCCCGAACTCACC30.1529051987767584No Hit
GCTCCATCTTGGCGATCAGGCGGGGCAGGGCGGTCTCGGAGCTGTCTCTT30.1529051987767584No Hit
GGTGGAGCACGGGCAGATCACGGGGTCGAGGCAGAAGATCGTGTGCTCGG30.1529051987767584No Hit
GCAGGGTCAGATGTGTATAAGAGACAGGCCCTGGACAGCTCCCCGCTGCA30.1529051987767584No Hit
GTGGACGCCACCGTCGGCATGACGGCCACGGACGAGGCCGTGGTCTCGAT30.1529051987767584No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGGATCTCGTATGCCGT30.1529051987767584No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGTCTT30.1529051987767584No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT30.1529051987767584Illumina PCR Primer Index 9 (95% over 22bp)
TCACCTGGTAGGTGATGCGCGACGGGGAGAAGACCATCCGGGACGCCAGC20.10193679918450561No Hit
GCGTCAGATGTGTATAAGAGACAGCCTCAGAAGGCGGCCACGGCCTGGTA20.10193679918450561No Hit
GCGCAAGGATCCCGGCATCAACGGCTGGGTGCTGAACTCGCCAACTAATC20.10193679918450561No Hit
CTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT20.10193679918450561RNA PCR Primer, Index 24 (95% over 22bp)
ACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAGTAC20.10193679918450561No Hit
TCTACGCGGAGGACGAGGACGACGACGCGCGGGCGCGATGACGCCCGCAC20.10193679918450561No Hit
GGCTGCGCGTGCTCCTGCTGGACCGCGGCCTGGACTCCCGGGCGCCGGAG20.10193679918450561No Hit
GCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTT20.10193679918450561No Hit
CACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACCACCTA20.10193679918450561No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCC20.10193679918450561No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGTC20.10193679918450561No Hit
ATCGTGTGGGGGAGCGCGCCCAGCGGACCGAACGACGCACCGCCCTTGAA20.10193679918450561No Hit
AGACGGACATGCTGGCCGACGAGTACCGCGACCCGCGGGGCGTGAAGCTG20.10193679918450561No Hit
GTAGGGGAAGGGAGCCTACTAGGGTGTAGAATAGGAAGTATGTGCCTGCG20.10193679918450561No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT20.10193679918450561No Hit
CTATTACTCTCATCGCTACCTCCCTGACAAGCGCCTATAGCACTCGAATA20.10193679918450561No Hit
CTGCTGGTGGACTCCGCCCCCACCGCGCCGCAGTTGGCGGGCTCCCTGAA20.10193679918450561No Hit
ATACATATGTTAAAATCACCAAGAATTGAGAGAGAAGAGTGACAGTAAAC20.10193679918450561No Hit
GGTTGCCCACCGAGGGGCCGCCGTCGCCGGAGAAGGTGTATGCCGTGTCC20.10193679918450561No Hit
GGGTTGGCTTGAAACCAGCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTC20.10193679918450561No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGCCGAGGCGGACTCGTCCCGT20.10193679918450561No Hit
CAGCTAGGGTATCAGGGCGACGGCGAGGTCGAGGAGATCGTCAACGAGGC20.10193679918450561No Hit
GGGTATGGGGTTAGCAGCGGTGTGTGTGTGCTGGGTAGGATGGGCGGGGG20.10193679918450561No Hit
GCCCTACAAGACGCTGGACGGCTACATCTGCGTCATCGTCTACAACGACA20.10193679918450561No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC20.10193679918450561No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT20.10193679918450561No Hit
GTTGTACGAGCGCACGGTGAGCTCGAGCTCCTCGATCGGCTGGGCCAGGT20.10193679918450561No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT20.10193679918450561TruSeq Adapter, Index 12 (95% over 23bp)
GAGAAGGAGGCCCCGCCCGGAAGCGGGATCGTCACCAAGCCCAGCAGGTA20.10193679918450561No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCTT20.10193679918450561No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT20.10193679918450561No Hit
ACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTT20.10193679918450561No Hit
TTTCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCT20.10193679918450561No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGC20.10193679918450561No Hit
CCTCGACGAGGAATGACAGGGCGTTTTCCGGTTTTCGACGCCACAGGCTA20.10193679918450561No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTCTT20.10193679918450561No Hit
GGCGGAGGTCGGCTCGTCCGCGATCAGCAGCTGCGGCCGGCACGCCAGGC20.10193679918450561No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTATAGCATCTCGTATGCCGT20.10193679918450561No Hit
GAAAGATTGTTGCCGCCGAAGTGGTGTACGAAGGTCGCTATCTCAAGCAG20.10193679918450561No Hit
CTCGGTAGGGGATTCCGCCGGATGTTTGGCGACGGTGGCGGAATCCAGAT20.10193679918450561No Hit
GGGCTCGGAGATGTGTATAAGAGACAGGTGGAGAACACCACGAGGCGCAC20.10193679918450561No Hit
GCCATCCCCGCGCGCACGGCCTCGGCCAGCTGCGCGTCGGAGACGGCGGA20.10193679918450561No Hit
ACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCT20.10193679918450561RNA PCR Primer, Index 24 (96% over 25bp)
TATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTAC20.10193679918450561No Hit
CAGTGTAATGGAATGGAGTGGAGTGGAGTGGTATGGAATTGAGTGGAATG20.10193679918450561No Hit
CCGGAGGCCTGCGCCATGAAGCCCAGGGCGGAGAACACGCCGATGCCGGC20.10193679918450561No Hit
GGTATAAGAGACAGCACATGACCAGCGAGCTCCTGCAGAACACGGTGGAG20.10193679918450561No Hit
ATGTAGACGTTCCAGAAGAACGGGATCAGCGACGCGCCGAGAACGAAGGA20.10193679918450561No Hit
GTTAAGGGTGGGTAGGTTTGTTGGTATCCTAGTGGGTGAGGGGTGGCTTT20.10193679918450561No Hit
CACGAAGGAGCCGCCGGACCACACGGCCGTGTTCAGGGAGGCGAACTTGT20.10193679918450561No Hit
GCAGCGTCAGATGTGTATAAGAGACAGAGAAGGAGGTGCCGGCCCAGTTC20.10193679918450561No Hit
ATGTTAGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAAT20.10193679918450561No Hit
GCAGCGTCAGATGTGTATAAGAGACAGCCCCGGAGGCGCTCGAGGGCGGC20.10193679918450561No Hit
CAGAGACGACGTCGGTCAGGCAGCCGAGCACGGGGATGCGGGCGTCCCGG20.10193679918450561No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT20.10193679918450561Illumina PCR Primer Index 9 (95% over 21bp)
GCGTCAGATGTGTATAAGAGACAGGTGGAGAAGGTCATGCGCGAGGTCTG20.10193679918450561No Hit
ACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGATAACCATACA20.10193679918450561No Hit
GCTGAGATCGGGCTCACATCCACTCGATCTCAGCGCCGCGCCCGACGGCG20.10193679918450561No Hit
GTATAAGAGACAGGCAGCGTCAGATGTGTATAAGAGCTGTCTCTTATACA20.10193679918450561No Hit
ATTGAGGAGTATCCTGAGGCATGGGGGTCAGGGGTTGAGGTCTTGGTGAG20.10193679918450561No Hit
GGTCTACCTGCTGGACGACTACCTGGGCGAGGGCGGCCGGGACAGCATCA20.10193679918450561No Hit
TTTATGGCGTCAGCGAAGGGTTGTAGTAGCCCGTAGGGGCCTACAACGTT20.10193679918450561No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT20.10193679918450561No Hit
GTGCTGAACGGCGCCAAGAAGTGGATCGGCAACGGGGCCGCCGGGGGCAT20.10193679918450561No Hit
TGCTAGGTGTGGTTGGTTGATGCCGATTGTAACTATTATGAGTCCTAGTT20.10193679918450561No Hit
CCACGGTGTGGGACGAGGACGGGCCGATGCCGATCGAGAACAGGTCGAGG20.10193679918450561No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC20.10193679918450561No Hit
TCAATGCATTGTGCGACATCGCAAACACCCCCCCGCACGCGTCTAAGGCC20.10193679918450561No Hit
GTTATTATTCTGAATTTTGGGGGAGGTTATATGGGTTTAATAGTTTTTTT20.10193679918450561No Hit
GAGGATGCCACGGGCATGGTGCCGTCCTTTCCGGAGAGCAGGGGCTCGGA20.10193679918450561No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG20.10193679918450561RNA PCR Primer, Index 34 (96% over 25bp)
GGTCAGGACGTCGCGGCGGTGTGCCGGGAGCTCGGGGTGTCCGAGCAGAC20.10193679918450561No Hit
CTCTCAGGCCGTGCGCGACGCCCTGCCGGAGCTGGCGTCCCTGGTGGGCG20.10193679918450561No Hit
CTAGCACAGAGAGTTCTCCCAGTAGGTTAATAGTGGGGGGTAAGGCGAGG20.10193679918450561No Hit
TACAAAACCCACCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTAC20.10193679918450561No Hit
GCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGTAT20.10193679918450561No Hit
GGATGGATAGTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGAT20.10193679918450561No Hit
ATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATATTGAGG20.10193679918450561No Hit
GGTCATTAGGAGGGCTGAGAGGGCCCCTGTTAGGGGTCATGGGCTGGGTT20.10193679918450561No Hit
GAACAGGGGGGTGCCGGCGGCGTCGGTCTTCAGCTCGGCCTGGTGGGGCA20.10193679918450561No Hit
GCGTCAGATGTGTATAAGAGACAGGCAAGCGTGCCACCTCCGCGACGTCG20.10193679918450561No Hit
ACCGCAAGGTGAGCGCGAGCGAGTGGGGCACGATCGCGGAGCGTTTCGTG20.10193679918450561No Hit
GTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTG20.10193679918450561No Hit
GCGCAAGGACATCGACCTCGTCCGCGAGCGCACGGATCTGAAGGCCGTCG20.10193679918450561No Hit
CAACTTCACCTCGTGGCTGCTGGAGACGCTCATGACGGAGATGCTCAGCG20.10193679918450561No Hit
AAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATATATGGTGTGC20.10193679918450561No Hit
CTCGCTCGCCGGCCAGGCCGCGTTGAAGTGAGATGACGGCGTGAAACGTC20.10193679918450561No Hit
GGCGGCCAGCGCACGCTCGTGCGAGCGGGCGGCGAAGGCGTCGGCGTCCT20.10193679918450561No Hit
CGAGATAGTAGTAGGGTCGTGGTGCTGGAGTTTAAGTTGAGTAGTAGGAA20.10193679918450561No Hit
TCATCCCATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACT20.10193679918450561No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC20.10193679918450561No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG20.10193679918450561No Hit
GCAGCGTCAGATGTGTATAAGAGACAGGCGGGGGACGGCTTCGACCCGGG20.10193679918450561No Hit
GTAGCCTAGGCTCGCGGCGAGGAAGCTTTCCAGCAGCCGGTGCCGGCGGA20.10193679918450561No Hit
GGATGCGGGGGAAATGTTGTTAGTAATGAGAAATCCTGCGAATAGGCTTC20.10193679918450561No Hit
CTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTTGA20.10193679918450561RNA PCR Primer, Index 24 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGCGTCCGAGCAGCTCGGCCGCCTCGTCC20.10193679918450561No Hit
ACGTTGCCGCGCTCGACCGCGCCCACCGCCTCGGCGAACCGCGTCATGAT20.10193679918450561No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT20.10193679918450561TruSeq Adapter, Index 2 (95% over 21bp)
GGTTTAAGCTCCTATTATTTACTCTATCAAAGTAACTCTTTTATCAGACA20.10193679918450561No Hit
TGCCTGTCCTCTGCACGCTCTGAACCTGCATCGCACGAGCGCGCATCTGG20.10193679918450561No Hit
GTTAGGTAGTTGAGGTCTAGGGCTGTTAGAAGTCCTAGGAAAGTGACAGC20.10193679918450561No Hit
TCCACGAGCACGGCGCCCAGCGCCACGGCCCAGGCGCGCGCGCCGGAGCG20.10193679918450561No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT20.10193679918450561No Hit
GACCGACGCTACCTCCGAGGACCAGCCGGACGACGTCCTGGGCCCGCTGC20.10193679918450561No Hit
GGTGGGGTGGATCGGGGTGCTGCCCGTCTGGTTGTACAGGGACACGTTGC20.10193679918450561No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT20.10193679918450561RNA PCR Primer, Index 30 (95% over 22bp)
GTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGTCAGGGAGGT20.10193679918450561No Hit
CTTATTCACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT20.10193679918450561No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC20.10193679918450561No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG405.0549716E-961.2536
TAGCATC405.0549716E-961.2533
ATAGCAT405.0549716E-961.2532
AGCATCT405.0549716E-961.2534
GCATCTC405.0549716E-961.2535
TATACAC600.058.3333323
TTATACA600.058.3333322
CTTATAC600.058.3333321
TGATAGC451.2842065E-854.44444330
AGACGTG451.2842065E-854.44444325
GACGTGA451.2842065E-854.44444326
GATAGCA451.2842065E-854.44444331
GAGACGT451.2842065E-854.44444324
ACGTGAT451.2842065E-854.44444327
AGCCCAC701.8189894E-1250.017
CACATCT701.8189894E-1250.07
CATCTCC701.8189894E-1250.09
GAGCCCA701.8189894E-1250.016
ACATCTC701.8189894E-1250.08
TACACAT701.8189894E-1250.05