##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779696_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11957
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.106297566279167	34.0	32.0	34.0	23.0	34.0
2	29.29020657355524	34.0	27.0	34.0	12.0	34.0
3	28.217027682529064	34.0	23.0	34.0	11.0	34.0
4	31.079535000418165	34.0	32.0	34.0	23.0	34.0
5	30.486326001505393	34.0	32.0	34.0	21.0	34.0
6	33.33687379777536	37.0	34.0	38.0	21.0	38.0
7	34.328594128962116	38.0	34.0	38.0	26.0	38.0
8	33.704190014217616	38.0	34.0	38.0	21.0	38.0
9	34.966797691728694	38.0	36.0	38.0	27.0	38.0
10-11	34.750397256837005	38.0	36.0	38.0	25.5	38.0
12-13	33.419628669398676	37.5	32.0	38.0	19.0	38.0
14-15	33.70055197792088	37.5	34.0	38.0	22.5	38.0
16-17	33.34264447603914	37.5	31.5	38.0	19.0	38.0
18-19	33.93438989713138	37.5	34.0	38.0	22.5	38.0
20-21	32.87697583005771	37.0	30.5	38.0	18.5	38.0
22-23	32.82332524880823	37.5	31.0	38.0	18.5	38.0
24-25	34.32508154219286	37.5	35.0	38.0	24.5	38.0
26-27	32.666429706448106	37.0	30.5	38.0	16.5	38.0
28-29	35.04378188508824	38.0	36.5	38.0	27.0	38.0
30-31	35.35853474951911	38.0	37.0	38.0	29.0	38.0
32-33	35.48005352513172	38.0	37.0	38.0	31.0	38.0
34-35	35.529355189428784	38.0	37.0	38.0	31.0	38.0
36-37	33.30743497532826	38.0	32.5	38.0	17.5	38.0
38-39	31.159739064982855	36.5	26.5	38.0	11.0	38.0
40-41	32.79957347160659	37.0	32.5	38.0	16.5	38.0
42-43	33.91611608262942	37.5	34.5	38.0	19.0	38.0
44-45	35.511708622564186	38.0	37.0	38.0	31.0	38.0
46-47	33.97838086476541	38.0	34.0	38.0	21.0	38.0
48-49	33.5206155390148	37.5	33.5	38.0	18.5	38.0
50-51	30.983858827465085	36.5	26.0	38.0	11.0	38.0
52-53	34.675252989880406	37.5	35.5	38.0	26.0	38.0
54-55	33.79208831646734	37.5	34.0	38.0	19.0	38.0
56-57	34.19917203311867	38.0	35.5	38.0	19.5	38.0
58-59	35.29890440746007	38.0	36.5	38.0	29.0	38.0
60-61	35.619469766663876	38.0	37.0	38.0	31.0	38.0
62-63	35.55398511332274	38.0	37.0	38.0	30.5	38.0
64-65	35.25499707284436	38.0	37.0	38.0	28.0	38.0
66-67	33.18286359454713	37.5	34.0	38.0	16.0	38.0
68-69	33.27669984109727	37.5	34.0	38.0	16.0	38.0
70-71	32.68951241950322	37.0	32.5	38.0	16.0	38.0
72-73	31.487998661871707	36.5	27.0	38.0	11.0	38.0
74-75	33.979760809567615	37.5	34.5	38.0	24.0	38.0
76	34.58124947729364	37.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	12.0
14	30.0
15	66.0
16	79.0
17	103.0
18	113.0
19	115.0
20	102.0
21	85.0
22	89.0
23	76.0
24	84.0
25	83.0
26	112.0
27	125.0
28	167.0
29	202.0
30	284.0
31	383.0
32	573.0
33	925.0
34	1497.0
35	2218.0
36	2776.0
37	1656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.814000167266038	23.89395333277578	13.030024253575313	31.26202224638287
2	17.63820356276658	17.7469264865769	40.94672576733294	23.668144183323577
3	16.74333026678933	19.645396002341727	35.07568788157565	28.535585849293298
4	11.524629923893952	19.059964874132305	36.305093250815425	33.110311951158316
5	11.332273981768003	33.637199966546795	39.18206908087313	15.848456970812078
6	24.02776616208079	36.33854645814167	17.972735636029107	21.660951743748434
7	23.684870786986703	28.88684452621895	23.701597390649827	23.72668729614452
8	19.09341808145856	35.21786401271222	19.92974826461487	25.758969641214353
9	20.398093167182406	15.112486409634524	21.97039391151627	42.51902651166681
10-11	21.65677009283265	25.94714393242452	28.535585849293298	23.860500125449526
12-13	21.422597641548883	22.334197541189262	28.957932591787237	27.285272225474618
14-15	20.598812411139917	24.663377101279586	24.466839508237854	30.270970979342643
16-17	21.159153633854647	26.214769591034543	27.147277745253827	25.478799029856987
18-19	20.159739064982855	26.599481475286442	27.226729112653675	26.014050347077028
20-21	20.782805051434305	26.528393409718156	27.465083214853227	25.223718323994316
22-23	21.43932424521201	25.227899974910095	28.196872125114997	25.1359036547629
24-25	19.17286944885841	27.628167600568705	26.99255666136991	26.206406289202977
26-27	19.377770343731704	25.855147612277328	28.93284268629255	25.83423935769842
28-29	19.783390482562517	27.983607928410137	27.47762816760057	24.75537342142678
30-31	19.540854729447187	27.582169440495107	26.796019068328174	26.080956761729528
32-33	19.40704190014218	27.406540102032285	27.009283265033034	26.177134732792506
34-35	20.163920715898637	27.005101614117255	27.005101614117255	25.825876055866857
36-37	20.858074767918374	25.236263276741656	29.434640796186333	24.471021159153633
38-39	20.728443589529146	24.68010370494271	32.048172618549806	22.54328008697834
40-41	19.30668227816342	26.055866856234843	26.461486995065652	28.175963870536087
42-43	18.487078698670235	26.612026428033786	27.26436397089571	27.63653090240027
44-45	18.829974073764323	27.778707033536843	27.494354771263694	25.896964121435147
46-47	20.481726185498033	26.13949987455047	27.782888684452622	25.595885255498874
48-49	20.352095007108804	26.256586100192358	28.485406038303925	24.905912854394916
50-51	21.95784895876892	25.06063393827883	29.61027013464916	23.371246968303087
52-53	19.68303086058376	27.904156561010286	27.297817178221962	25.114995400183993
54-55	20.31027849795099	27.327088734632433	27.360541941958683	25.00209082545789
56-57	20.84552981517103	26.361127373086894	27.306180480053527	25.487162331688552
58-59	19.273229070837168	27.908338211926072	27.770343731705278	25.048088985531486
60-61	19.7875721334783	26.11022831814	28.301413398009533	25.80078615037217
62-63	17.76783474115581	27.75361712804215	28.297231747093754	26.181316383708285
64-65	19.80011708622564	28.242870285188594	26.796019068328174	25.160993560257587
66-67	18.332357614786318	25.98059713975077	25.223718323994316	30.463326921468596
68-69	18.06281627702731	26.59445443519719	25.97131027560537	29.37141901217013
70-71	18.74634105544869	26.177134732792506	25.930417328761397	29.146106882997408
72-73	18.487078698670235	25.031362381868362	24.429204649995818	32.05235426946558
74-75	18.616709877059463	27.837250146357782	26.908923643054276	26.637116333528475
76	19.33595383457389	27.45671991302166	27.465083214853227	25.742243037551226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	3.0
17	5.0
18	3.0
19	1.0
20	3.5
21	6.0
22	7.5
23	10.5
24	15.5
25	19.0
26	26.5
27	47.5
28	71.5
29	82.0
30	110.5
31	155.5
32	195.0
33	218.0
34	255.5
35	329.5
36	410.0
37	454.0
38	490.5
39	575.5
40	679.0
41	751.0
42	768.0
43	767.0
44	777.5
45	739.0
46	689.0
47	724.0
48	741.0
49	652.0
50	581.0
51	553.0
52	480.5
53	391.0
54	346.0
55	314.0
56	251.0
57	206.5
58	193.0
59	167.0
60	143.0
61	117.0
62	89.0
63	99.5
64	97.5
65	83.5
66	82.5
67	83.0
68	75.0
69	68.0
70	56.5
71	44.0
72	37.5
73	37.5
74	40.0
75	36.0
76	32.0
77	25.5
78	20.5
79	18.0
80	14.0
81	8.0
82	4.5
83	3.0
84	2.5
85	2.0
86	2.0
87	2.0
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.012544952747344651
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	11957.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.21142427030192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.95757997218358	93.28426862925483
2	2.5034770514603615	4.817261854980346
3	0.35639777468706535	1.0286861252822614
4	0.09561891515994438	0.36798528058877644
5	0.03477051460361613	0.16726603663126202
6	0.03477051460361613	0.20071924395751442
7	0.008692628650904033	0.05854311282094171
8	0.0	0.0
9	0.008692628650904033	0.07526971648406791
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.008363301831563101	0.0
20	0.0	0.0	0.0	0.008363301831563101	0.0
21	0.0	0.0	0.0	0.008363301831563101	0.0
22	0.0	0.0	0.0	0.016726603663126203	0.0
23	0.0	0.0	0.0	0.016726603663126203	0.0
24	0.0	0.0	0.0	0.033453207326252406	0.0
25	0.0	0.0	0.0	0.033453207326252406	0.0
26	0.0	0.0	0.0	0.033453207326252406	0.0
27	0.0	0.0	0.0	0.033453207326252406	0.0
28	0.0	0.0	0.0	0.041816509157815505	0.0
29	0.0	0.0	0.0	0.041816509157815505	0.0
30	0.0	0.0	0.0	0.041816509157815505	0.0
31	0.0	0.0	0.0	0.041816509157815505	0.0
32	0.0	0.0	0.0	0.041816509157815505	0.0
33	0.0	0.0	0.0	0.058543112820941705	0.0
34	0.0	0.0	0.0	0.058543112820941705	0.0
35	0.0	0.0	0.0	0.07526971648406791	0.0
36	0.0	0.0	0.0	0.09199632014719411	0.0
37	0.0	0.0	0.0	0.10035962197875721	0.0
38	0.0	0.0	0.0	0.11708622564188341	0.0
39	0.0	0.0	0.0	0.18399264029438822	0.0
40	0.0	0.0	0.0	0.20071924395751442	0.0
41	0.0	0.0	0.0	0.250899054946893	0.0
42	0.0	0.0	0.0	0.29271556410470856	0.0
43	0.0	0.0	0.0	0.35962197875721336	0.0
44	0.0	0.0	0.0	0.39307518608346576	0.0
45	0.0	0.0	0.0	0.45998160073597055	0.0
46	0.0	0.0	0.0	0.5268880153884754	0.0
47	0.0	0.0	0.0	0.5519779208831647	0.0
48	0.0	0.0	0.0	0.6021577318725433	0.0
49	0.0	0.0	0.0	0.6356109391987956	0.0
50	0.0	0.0	0.0	0.7025173538513005	0.0
51	0.0	0.0	0.0	0.7694237685038053	0.0
52	0.0	0.0	0.0	0.7861503721669315	0.0
53	0.0	0.0	0.0	0.911599899640378	0.0
54	0.0	0.0	0.0	0.9868696161244459	0.0
55	0.0	0.0	0.0	1.0537760307769508	0.0
56	0.0	0.0	0.0	1.07886593627164	0.0
57	0.0	0.0	0.0	1.1374090490925817	0.0
58	0.0	0.0	0.0	1.1792255582503972	0.0
59	0.0	0.0	0.0	1.254495274734465	0.0
60	0.0	0.0	0.0	1.2712218783975913	0.0
61	0.0	0.0	0.0	1.2795851802291545	0.0
62	0.0	0.0	0.0	1.3046750857238438	0.0
63	0.0	0.0	0.0	1.3715815003763485	0.0
64	0.0	0.0	0.0	1.4301246131972902	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTAG	20	0.0067857113	52.5	30
GTAGTTA	20	0.0067857113	52.5	29
>>END_MODULE