FastQCFastQC Report
Wed 25 May 2016
SRR1779696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779696_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11957
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT880.7359705611775529No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT620.5185247135569122TruSeq Adapter, Index 12 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC410.34289537509408713RNA PCR Primer, Index 12 (95% over 21bp)
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG240.20071924395751442No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC230.1923559421259513TruSeq Adapter, Index 12 (95% over 21bp)
ATATAGGGTCGAAGCCGCACTCGTAAGGGGTGGATTTTTCTATGTAGCCG150.1254495274734465No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG150.1254495274734465No Hit
CTATAGTTGCAAGCAGGAGGATAATGCCGATGTTTCAGGTTTCTGAGTAG140.11708622564188342No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC130.10872292381032031No Hit
GAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTC130.10872292381032031No Hit
GATTAGTAGTATGGGAGTGGGAGGGGAAAATAATGTGTTAGTTGGGGGGT130.10872292381032031No Hit
GATGAGGATGGATAGTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGA130.10872292381032031No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT130.10872292381032031No Hit
ATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCTA120.10035962197875721No Hit
GGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTAC120.10035962197875721No Hit
ATATAGGATTTAGCCTATAATTTAACTTTGACAAAGTTATGAAATGGTTT120.10035962197875721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA150.002171268570.068
GGAAGAG200.006785711352.524
TATACAC350.001206572640.03
TTATACA350.001206572640.02
CTTATAC350.001206572640.01
TAGGCCT350.001206572640.04
CCGTCTT501.6115299E-435.044
GTCTTCT501.6115299E-435.046
TCTTCTG501.6115299E-435.047
CTACTTG400.00232520935.025
CTTCTGC501.6115299E-435.048
GTATGCC552.821116E-431.8181839
ATCTCGT552.821116E-431.8181834
TTCTGCT552.821116E-431.8181849
TCTCGTA552.821116E-431.8181835
ACGAGAC552.821116E-431.8181819
CGTATGC552.821116E-431.8181838
CTGCTTG552.821116E-431.8181851
TCGTATG552.821116E-431.8181837
TTGAAAA552.821116E-431.8181855