##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779696_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11957
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93610437400686	34.0	34.0	34.0	31.0	34.0
2	33.281926904741994	34.0	34.0	34.0	32.0	34.0
3	33.29371916032449	34.0	34.0	34.0	33.0	34.0
4	33.40210755206155	34.0	34.0	34.0	34.0	34.0
5	33.34473530149703	34.0	34.0	34.0	33.0	34.0
6	36.53525131722004	38.0	37.0	38.0	34.0	38.0
7	36.582838504641636	38.0	37.0	38.0	34.0	38.0
8	36.173538513004935	38.0	37.0	38.0	34.0	38.0
9	35.896545956343566	38.0	36.0	38.0	32.0	38.0
10-11	36.04311282094171	38.0	36.5	38.0	32.5	38.0
12-13	36.1059630342059	38.0	37.0	38.0	33.5	38.0
14-15	36.129380279334285	38.0	37.0	38.0	33.0	38.0
16-17	36.09856151208497	38.0	36.5	38.0	33.0	38.0
18-19	36.09270720080288	38.0	36.5	38.0	33.0	38.0
20-21	36.13970895709626	38.0	37.0	38.0	34.0	38.0
22-23	35.92113406372836	38.0	36.0	38.0	32.0	38.0
24-25	35.965459563435644	38.0	36.0	38.0	32.0	38.0
26-27	35.846491594881655	38.0	36.0	38.0	31.5	38.0
28-29	35.73141256167935	38.0	36.0	38.0	31.5	38.0
30-31	35.63101112319144	38.0	36.0	38.0	31.0	38.0
32-33	35.67847286108555	38.0	36.0	38.0	31.0	38.0
34-35	35.62674583925734	38.0	36.0	38.0	31.0	38.0
36-37	35.66847871539684	38.0	36.0	38.0	31.0	38.0
38-39	35.852722254746176	38.0	36.0	38.0	32.0	38.0
40-41	35.79058292213766	38.0	36.0	38.0	31.5	38.0
42-43	35.77774525382621	38.0	36.0	38.0	31.0	38.0
44-45	35.740612193694076	38.0	36.0	38.0	31.0	38.0
46-47	35.85322405285606	38.0	36.0	38.0	31.5	38.0
48-49	35.78899389478966	38.0	36.0	38.0	31.5	38.0
50-51	35.775194446767586	38.0	36.0	38.0	31.0	38.0
52-53	35.775487162331686	38.0	36.0	38.0	31.0	38.0
54-55	35.81199297482647	38.0	36.0	38.0	31.5	38.0
56-57	35.735719662122605	38.0	36.0	38.0	31.0	38.0
58-59	35.73082713055114	38.0	36.0	38.0	31.0	38.0
60-61	35.729907167349666	38.0	36.0	38.0	31.0	38.0
62-63	35.62846031613281	38.0	36.0	38.0	31.0	38.0
64-65	35.548172618549806	38.0	36.0	38.0	31.0	38.0
66-67	35.510412310780296	38.0	36.0	38.0	31.0	38.0
68-69	35.455590867274395	38.0	36.0	38.0	31.0	38.0
70-71	35.198001170862256	38.0	36.0	38.0	28.0	38.0
72-73	35.15610102868612	38.0	36.0	38.0	27.0	38.0
74-75	35.19553399682194	38.0	36.0	38.0	27.0	38.0
76	34.01597390649829	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	8.0
24	11.0
25	13.0
26	31.0
27	47.0
28	60.0
29	122.0
30	190.0
31	378.0
32	507.0
33	815.0
34	1313.0
35	1943.0
36	2815.0
37	3704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91327256000669	24.504474366479887	12.846031613280923	21.7362214602325
2	22.020573722505645	21.861670987705946	35.79493183909007	20.322823450698337
3	19.344317136405454	27.490173120347915	30.994396587772854	22.171113155473783
4	15.689554236012377	18.54980346240696	41.50706699004767	24.253575311532995
5	15.263025842602659	38.92280672409467	33.637199966546795	12.176967466755876
6	30.668227816341894	37.149786735803296	18.90942544116417	13.272560006690643
7	29.580998578238688	30.166429706448106	24.069582671238603	16.1829890440746
8	25.206991720331185	36.34690975997324	20.080287697583003	18.365810822112568
9	26.96328510495944	18.022915447018484	22.530735134230994	32.48306431379108
10-11	27.45671991302166	26.039140252571713	27.536171280421513	18.967968553985113
12-13	27.18491260349586	22.823450698335705	26.8127456719913	23.178891026177133
14-15	25.579158651835744	24.471021159153633	23.697415739734044	26.252404449276572
16-17	26.23149619469767	27.80379693903153	24.395751442669567	21.56895542360124
18-19	25.144266956594464	27.306180480053527	26.273312703855485	21.276239859496528
20-21	24.742828468679434	27.059463076022418	25.750606339382788	22.447102115915364
22-23	26.419670485907837	27.034373170527726	25.750606339382788	20.79535000418165
24-25	24.713556912268963	27.88324830643138	26.57020991887597	20.832984862423682
26-27	24.4333863009116	28.12996571046249	27.20582085807477	20.23082713055114
28-29	24.28702851885925	27.435811658442756	28.28468679434641	19.992473028351593
30-31	24.291210169775027	26.842017228401772	27.866521702768253	21.000250899054947
32-33	24.094672576733295	27.920883164673416	27.782888684452622	20.20155557414067
34-35	25.595885255498874	27.88742995734716	27.573806138663542	18.942878648490424
36-37	24.203395500543614	28.20941707786234	27.285272225474618	20.301915196119428
38-39	24.684285355858492	27.113824537927574	27.603077695074013	20.598812411139917
40-41	24.333026678932843	26.0433219034875	28.840846366145357	20.782805051434305
42-43	24.93936606172117	26.482395249644558	29.008112402776614	19.570126285857654
44-45	23.94413314376516	27.908338211926072	27.736890524379028	20.410638119929747
46-47	25.36589445513088	27.013464915948816	26.770929162833486	20.849711466086813
48-49	24.847369741573974	25.809149452203727	27.88324830643138	21.46023249979092
50-51	23.822865267207494	26.917286944885845	27.423266705695408	21.836581082211257
52-53	24.10721752948064	26.172953081876727	29.305009617797108	20.41481977084553
54-55	24.270301915196118	26.733294304591453	28.711215187756128	20.2851885924563
56-57	24.583925733879735	26.118591619971564	28.45195283097767	20.84552981517103
58-59	24.50865601739567	27.276908923643056	28.34322990716735	19.871205151793927
60-61	24.56719913021661	27.85397675002091	27.778707033536843	19.80011708622564
62-63	22.948900225809147	29.572635276407127	27.092916283348668	20.385548214435058
64-65	23.488333193944968	30.701681023668144	25.600066906414654	20.20991887597223
66-67	23.32943045914527	29.953165509743247	26.78347411558083	19.93392991553065
68-69	22.597641548883498	31.529647904992892	25.88860081960358	19.984109726520032
70-71	23.676507485155142	29.685539851133225	26.499121853307685	20.13883081040395
72-73	23.65978088149201	28.46449778372501	26.56602826796019	21.30969306682278
74-75	23.496696495776533	29.26737475955507	26.56602826796019	20.669900476708207
76	23.609601070502634	29.580998578238688	27.013464915948816	19.79593543530986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.5
16	2.0
17	3.0
18	2.0
19	2.5
20	5.0
21	6.0
22	8.5
23	24.5
24	43.0
25	48.0
26	50.0
27	61.5
28	92.5
29	114.0
30	136.5
31	161.0
32	217.5
33	272.0
34	278.5
35	330.5
36	395.5
37	415.0
38	519.0
39	681.5
40	781.0
41	786.5
42	751.0
43	821.0
44	848.5
45	779.0
46	752.0
47	740.0
48	683.5
49	584.5
50	530.0
51	486.0
52	415.5
53	351.5
54	314.0
55	285.5
56	230.0
57	158.5
58	114.0
59	101.5
60	88.0
61	82.5
62	78.0
63	72.5
64	69.0
65	64.0
66	56.5
67	56.0
68	59.0
69	76.0
70	68.0
71	46.0
72	41.5
73	39.5
74	41.5
75	41.0
76	35.5
77	26.5
78	22.0
79	21.0
80	18.0
81	12.5
82	7.5
83	5.0
84	5.5
85	4.0
86	1.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	11957.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.13222380195701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78510028653295	50.2049008948733
2	15.136414600722562	20.322823450698337
3	5.469041983306341	11.014468512168603
4	1.9808147502180142	5.319059964874132
5	1.1835056683692537	3.9725683699924725
6	0.5606079481749097	2.2580914945220374
7	0.3737386321166064	1.7562933846282511
8	0.08720568082720817	0.4683449025675337
9	0.09966363523109506	0.6021577318725433
>10	0.2989909056932851	2.826796019068328
>50	0.024915908807773765	1.254495274734465
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	88	0.7359705611775529	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	62	0.5185247135569122	TruSeq Adapter, Index 12 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	41	0.34289537509408713	RNA PCR Primer, Index 12 (95% over 21bp)
GTATTAGGAGGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAG	24	0.20071924395751442	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	23	0.1923559421259513	TruSeq Adapter, Index 12 (95% over 21bp)
ATATAGGGTCGAAGCCGCACTCGTAAGGGGTGGATTTTTCTATGTAGCCG	15	0.1254495274734465	No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG	15	0.1254495274734465	No Hit
CTATAGTTGCAAGCAGGAGGATAATGCCGATGTTTCAGGTTTCTGAGTAG	14	0.11708622564188342	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	13	0.10872292381032031	No Hit
GAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTC	13	0.10872292381032031	No Hit
GATTAGTAGTATGGGAGTGGGAGGGGAAAATAATGTGTTAGTTGGGGGGT	13	0.10872292381032031	No Hit
GATGAGGATGGATAGTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGA	13	0.10872292381032031	No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT	13	0.10872292381032031	No Hit
ATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAGACCTA	12	0.10035962197875721	No Hit
GGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTAC	12	0.10035962197875721	No Hit
ATATAGGATTTAGCCTATAATTTAACTTTGACAAAGTTATGAAATGGTTT	12	0.10035962197875721	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.025089905494689303	0.0
20	0.0	0.0	0.0	0.025089905494689303	0.0
21	0.0	0.0	0.0	0.025089905494689303	0.0
22	0.0	0.0	0.0	0.025089905494689303	0.0
23	0.0	0.0	0.0	0.025089905494689303	0.0
24	0.0	0.0	0.0	0.025089905494689303	0.0
25	0.0	0.0	0.0	0.025089905494689303	0.0
26	0.0	0.0	0.0	0.025089905494689303	0.0
27	0.0	0.0	0.0	0.025089905494689303	0.0
28	0.0	0.0	0.0	0.033453207326252406	0.0
29	0.0	0.0	0.0	0.050179810989378605	0.0
30	0.0	0.0	0.0	0.050179810989378605	0.0
31	0.0	0.0	0.0	0.06690641465250481	0.0
32	0.0	0.0	0.0	0.07526971648406791	0.0
33	0.0	0.0	0.0	0.09199632014719411	0.0
34	0.0	0.0	0.0	0.09199632014719411	0.0
35	0.0	0.0	0.0	0.09199632014719411	0.0
36	0.0	0.0	0.0	0.1254495274734465	0.0
37	0.0	0.0	0.0	0.1589027347996989	0.0
38	0.0	0.0	0.0	0.20908254578907753	0.0
39	0.0	0.0	0.0	0.29271556410470856	0.0
40	0.0	0.0	0.0	0.33453207326252404	0.0
41	0.0	0.0	0.0	0.40143848791502884	0.0
42	0.0	0.0	0.0	0.43489169524128124	0.0
43	0.0	0.0	0.0	0.5268880153884754	0.0
44	0.0	0.0	0.0	0.5687045245462908	0.0
45	0.0	0.0	0.0	0.6690641465250481	0.0
46	0.0	0.0	0.0	0.7861503721669315	0.0
47	0.0	0.0	0.0	0.8363301831563101	0.0
48	0.0	0.0	0.0	0.9283265033035042	0.0
49	0.0	0.0	0.0	1.0286861252822614	0.0
50	0.0	0.0	0.0	1.1374090490925817	0.0
51	0.0	0.0	0.0	1.2043154637450866	0.0
52	0.0	0.0	0.0	1.254495274734465	0.0
53	0.0	0.0	0.0	1.3715815003763485	0.0
54	0.0	0.0	0.0	1.480304424186669	0.0
55	0.0	0.0	0.0	1.5973906498285524	0.0
56	0.0	0.0	0.0	1.6392071589863677	0.0
57	0.0	0.0	0.0	1.7061135736388726	0.0
58	0.0	0.0	0.0	1.8064731956176299	0.0
59	0.0	0.0	0.0	1.9402860249226395	0.0
60	0.0	0.0	0.0	2.057372250564523	0.0
61	0.0	0.0	0.0	2.141005268880154	0.0
62	0.0	0.0	0.0	2.2079116835326587	0.0
63	0.0	0.0	0.0	2.324997909174542	0.0
64	0.0	0.0	0.0	2.4337208329848625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCTCAA	15	0.0021712685	70.0	68
GGAAGAG	20	0.0067857113	52.5	24
TATACAC	35	0.0012065726	40.0	3
TTATACA	35	0.0012065726	40.0	2
CTTATAC	35	0.0012065726	40.0	1
TAGGCCT	35	0.0012065726	40.0	4
CCGTCTT	50	1.6115299E-4	35.0	44
GTCTTCT	50	1.6115299E-4	35.0	46
TCTTCTG	50	1.6115299E-4	35.0	47
CTACTTG	40	0.002325209	35.0	25
CTTCTGC	50	1.6115299E-4	35.0	48
GTATGCC	55	2.821116E-4	31.81818	39
ATCTCGT	55	2.821116E-4	31.81818	34
TTCTGCT	55	2.821116E-4	31.81818	49
TCTCGTA	55	2.821116E-4	31.81818	35
ACGAGAC	55	2.821116E-4	31.81818	19
CGTATGC	55	2.821116E-4	31.81818	38
CTGCTTG	55	2.821116E-4	31.81818	51
TCGTATG	55	2.821116E-4	31.81818	37
TTGAAAA	55	2.821116E-4	31.81818	55
>>END_MODULE