##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779695_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14387
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.915062208938625	32.0	23.0	34.0	12.0	34.0
2	26.65920622784458	32.0	21.0	34.0	12.0	34.0
3	25.97462987419198	31.0	21.0	34.0	11.0	34.0
4	28.56398137207201	32.0	23.0	34.0	12.0	34.0
5	27.951205949815805	32.0	23.0	34.0	11.0	34.0
6	29.565023979981927	34.0	24.0	37.0	11.0	38.0
7	30.763953569194413	34.0	27.0	37.0	12.0	38.0
8	30.261069020643635	34.0	26.0	37.0	11.0	38.0
9	32.11315771182317	36.0	31.0	38.0	12.0	38.0
10-11	31.608257454646555	36.0	29.0	38.0	11.0	38.0
12-13	30.603982762215885	35.5	26.5	37.5	11.0	38.0
14-15	30.734552026134704	35.0	26.5	37.5	11.5	38.0
16-17	30.501320636685897	35.0	26.5	37.5	11.0	38.0
18-19	30.836901369291724	35.5	26.5	37.5	11.5	38.0
20-21	29.9089455758671	34.5	24.0	37.0	11.0	38.0
22-23	29.934802252033087	35.0	26.0	37.5	11.0	38.0
24-25	31.44064085632863	35.0	29.0	37.5	16.0	38.0
26-27	29.636685896990336	34.0	23.5	37.0	11.0	38.0
28-29	32.012893584485994	36.5	29.5	38.0	11.0	38.0
30-31	32.77093904219086	37.0	31.5	38.0	21.0	38.0
32-33	33.064120386460004	37.0	34.0	38.0	21.0	38.0
34-35	33.118718287342745	37.0	34.0	38.0	21.0	38.0
36-37	30.31076666434976	35.0	25.0	38.0	11.0	38.0
38-39	27.988496559393898	33.0	21.0	37.0	11.0	38.0
40-41	29.378119135330508	34.0	24.5	37.0	11.0	38.0
42-43	30.835511225411828	35.0	27.5	37.0	16.0	38.0
44-45	32.98776673385696	37.0	33.0	38.0	21.0	38.0
46-47	31.235386112462642	35.5	28.5	38.0	16.0	38.0
48-49	30.23924376172934	35.0	24.5	37.0	16.0	38.0
50-51	27.681344269131856	32.5	21.0	37.0	11.0	38.0
52-53	31.565580037533884	35.0	28.5	37.0	16.0	38.0
54-55	30.6384930840342	35.0	27.0	37.5	15.0	38.0
56-57	31.292173489956213	36.0	28.0	38.0	16.0	38.0
58-59	32.64829359838743	36.5	31.5	38.0	21.0	38.0
60-61	33.300027802877594	37.0	34.0	38.0	22.0	38.0
62-63	33.17665253353722	37.0	33.5	38.0	21.5	38.0
64-65	32.80120942517551	37.0	32.5	38.0	16.0	38.0
66-67	30.2794884270522	35.0	24.5	37.5	11.0	38.0
68-69	30.127649961771045	34.5	25.0	37.0	11.0	38.0
70-71	29.54191283797873	34.0	24.0	37.0	11.0	38.0
72-73	28.14193369013693	33.0	21.5	37.0	11.0	38.0
74-75	30.46590672134566	35.0	26.0	37.0	15.0	38.0
76	30.824285813581707	34.0	27.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	10.0
14	23.0
15	35.0
16	61.0
17	92.0
18	160.0
19	208.0
20	215.0
21	257.0
22	290.0
23	342.0
24	365.0
25	418.0
26	521.0
27	539.0
28	699.0
29	837.0
30	1009.0
31	1145.0
32	1345.0
33	1597.0
34	1589.0
35	1437.0
36	980.0
37	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.649336206297356	20.90081323416974	11.517342044901648	32.932508514631266
2	17.418502815041357	17.077917564467924	42.496698408285255	23.006881212205464
3	16.76513519149232	16.848543824285812	40.68951136442622	25.696809619795648
4	12.358379092236046	19.489817196079795	35.031625773267535	33.12017793841663
5	11.385278376311948	30.346840898032944	42.24647250990478	16.02140821575033
6	25.272815736428722	37.658997706262596	17.230833391255995	19.837353166052687
7	26.45443803433655	27.96274414401891	20.622784458191422	24.960033363453118
8	21.741850281504135	34.489469660109826	18.815597414332384	24.95308264405366
9	23.284909988183777	15.673872245777437	20.08757906443317	40.95363870160561
10-11	22.947800097310072	24.970459442552304	28.324181552790712	23.75755890734691
12-13	21.689719886008202	21.005074025161605	31.84819628831584	25.457009800514353
14-15	23.034684089803296	24.14679919371655	23.861819698338778	28.956697018141377
16-17	25.17898102453604	24.258010704107875	25.93313407937722	24.62987419197887
18-19	19.941613957044556	24.78626537846667	29.095711406130537	26.17640925835824
20-21	24.53256412038646	24.71675818447209	26.17640925835824	24.574268436783207
22-23	24.66462778897616	23.20845207478974	27.583929936748454	24.542990199485647
24-25	20.292625286717175	27.215541808577186	25.81497184958643	26.676861055119204
26-27	23.37526934037673	25.140752067839024	27.201640369778275	24.28233822200598
28-29	21.422117189129075	26.795023284909984	26.742892889414055	25.039966636546886
30-31	21.005074025161605	27.139083895183152	26.513519149231946	25.3423229304233
32-33	21.196218808646694	26.7845972058108	26.583026343226525	25.436157642315983
34-35	21.293528880239105	26.78807256551053	26.88190727740321	25.036491276847155
36-37	23.340515743379438	24.011260165427124	29.856815180371164	22.79140891082227
38-39	23.13894488079516	22.53423229304233	34.322652394522834	20.004170431639675
40-41	21.547230138319314	26.27371932995065	25.199833182734416	26.979217348995622
42-43	19.913811079446724	27.587405296448182	26.072148467366375	26.42663515673872
44-45	20.10843122263154	25.93313407937722	28.320706193090984	25.63772850490026
46-47	23.542086605963718	24.324042538402722	27.594356015847637	24.539514839785916
48-49	23.75408354764718	25.279766455828177	27.034823104191286	23.931326892333356
50-51	27.875860151525682	22.204073121568083	27.830680475429208	22.089386251477027
52-53	20.31000208521582	26.54132202682978	27.841106554528395	25.30756933342601
54-55	22.82963786751929	25.130325988739834	27.615208174046014	24.424827969694864
56-57	21.66191700841037	25.335372211023845	28.028775978313757	24.97393480225203
58-59	20.05282546743588	26.593452422325708	27.76464864113436	25.58907346910405
60-61	20.296100646416903	26.680336414818935	27.594356015847637	25.42920692291652
62-63	19.934663237645093	26.43358587613818	27.809828317230835	25.82192256898589
64-65	20.02502258983805	28.01139917981511	27.187738930979354	24.775839299367487
66-67	18.714811983040246	28.233822200597764	24.511711962188087	28.539653854173906
68-69	18.37550396218546	28.367857639371614	25.507437786737107	27.749200611705827
70-71	18.325571696670607	28.185167164801555	24.69938138597345	28.789879752554388
72-73	17.995412525196357	28.873288385347884	23.04163480920275	30.089664280253004
74-75	19.70528949746299	27.938416626120805	25.825397928685618	26.530895947730592
76	20.970320428164317	26.586501702926252	26.398832279140894	26.044345589768543
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	1.5
14	1.0
15	1.0
16	2.5
17	5.0
18	3.5
19	5.5
20	7.5
21	6.0
22	8.5
23	16.5
24	28.0
25	34.0
26	49.0
27	74.5
28	92.0
29	99.0
30	121.5
31	179.5
32	235.0
33	255.0
34	307.5
35	393.0
36	474.0
37	522.0
38	555.5
39	648.5
40	768.5
41	831.0
42	833.0
43	845.0
44	879.0
45	855.0
46	809.0
47	823.5
48	788.5
49	716.5
50	694.0
51	648.0
52	565.5
53	477.5
54	426.0
55	370.5
56	307.0
57	280.5
58	262.0
59	240.0
60	201.0
61	163.5
62	143.0
63	147.0
64	153.0
65	150.5
66	128.0
67	110.0
68	103.5
69	103.0
70	107.5
71	106.0
72	96.0
73	86.5
74	77.5
75	68.0
76	64.0
77	53.5
78	42.5
79	38.0
80	36.0
81	30.0
82	18.0
83	10.0
84	9.0
85	4.5
86	2.0
87	3.0
88	2.5
89	1.5
90	0.5
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.006950719399457845
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	14387.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.25627302425801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.34173669467788	98.60290540070898
2	0.5742296918767508	1.1399179815110865
3	0.07703081232492998	0.22937374018210888
4	0.007002801120448179	0.02780287759783138
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.006950719399457844	0.0
20	0.0	0.0	0.0	0.013901438798915687	0.0
21	0.0	0.0	0.0	0.013901438798915687	0.0
22	0.0	0.0	0.0	0.03475359699728922	0.0
23	0.0	0.0	0.0	0.041704316396747064	0.0
24	0.0	0.0	0.0	0.041704316396747064	0.0
25	0.0	0.0	0.0	0.041704316396747064	0.0
26	0.0	0.0	0.0	0.041704316396747064	0.0
27	0.0	0.0	0.0	0.041704316396747064	0.0
28	0.0	0.0	0.0	0.041704316396747064	0.0
29	0.0	0.0	0.0	0.041704316396747064	0.0
30	0.0	0.0	0.0	0.041704316396747064	0.0
31	0.0	0.0	0.0	0.041704316396747064	0.0
32	0.0	0.0	0.0	0.041704316396747064	0.0
33	0.0	0.0	0.0	0.041704316396747064	0.0
34	0.0	0.0	0.0	0.04865503579620491	0.0
35	0.0	0.0	0.0	0.04865503579620491	0.0
36	0.0	0.0	0.0	0.05560575519566275	0.0
37	0.0	0.0	0.0	0.06950719399457844	0.0
38	0.0	0.0	0.0	0.09035935219295196	0.0
39	0.0	0.0	0.0	0.1112115103913255	0.0
40	0.0	0.0	0.0	0.13206366858969903	0.0
41	0.0	0.0	0.0	0.16681726558698826	0.0
42	0.0	0.0	0.0	0.20852158198373533	0.0
43	0.0	0.0	0.0	0.29193021477722947	0.0
44	0.0	0.0	0.0	0.35448668937235006	0.0
45	0.0	0.0	0.0	0.43789532216584415	0.0
46	0.0	0.0	0.0	0.4865503579620491	0.0
47	0.0	0.0	0.0	0.5838604295544589	0.0
48	0.0	0.0	0.0	0.667269062347953	0.0
49	0.0	0.0	0.0	0.7367762563425314	0.0
50	0.0	0.0	0.0	0.8549384861333148	0.0
51	0.0	0.0	0.0	1.0356571905192187	0.0
52	0.0	0.0	0.0	1.1746715785083757	0.0
53	0.0	0.0	0.0	1.3901438798915688	0.0
54	0.0	0.0	0.0	1.5222075484812678	0.0
55	0.0	0.0	0.0	1.647320497671509	0.0
56	0.0	0.0	0.0	1.7515812886633766	0.0
57	0.0	0.0	0.0	1.8488913602557864	0.0
58	0.0	0.0	0.0	1.9879057482449434	0.0
59	0.0	0.0	0.0	2.1269201362341	0.0
60	0.0	0.0	0.0	2.217279488427052	0.0
61	0.0	0.0	0.0	2.2937374018210885	0.0
62	0.0	0.0	0.0	2.363244595815667	0.0
63	0.0	0.0	0.0	2.488357545005908	0.0
64	0.0	0.0	0.0	2.6204212135956073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGCA	15	0.002183294	69.97562	48
CTATTCC	15	0.002183294	69.97562	28
>>END_MODULE