FastQCFastQC Report
Wed 25 May 2016
SRR1779695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14387
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT1080.7506776951414471TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT280.19462014318481963TruSeq Adapter, Index 5 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG150.002180278870.036
TTAGGCT150.002180278870.03
AAAAATT150.002180278870.067
CCGTCTT252.3120898E-455.99999647
CTGCTTG252.3120898E-455.99999654
CGTCTTC252.3120898E-455.99999648
ATCTCGT200.006813654752.537
TGATCTC200.006813654752.535
GTGATCT200.006813654752.534
AAGTGAT200.006813654752.532
TCTGCTT351.9852818E-550.00000453
TGCCGTC305.686422E-446.66666445
TATGCCG305.686422E-446.66666443
TTCTGCT305.686422E-446.66666452
ATGCCGT305.686422E-446.66666444
CGTATGC305.686422E-446.66666441
GCTTGAA305.686422E-446.66666456
TCGTATG305.686422E-446.66666440
GCCGTCT305.686422E-446.66666446
CGAGACA305.686422E-446.66666423
Produced by FastQC (version 0.11.5)