##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779695_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14387
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00938347118927	34.0	34.0	34.0	31.0	34.0
2	33.2999235420866	34.0	34.0	34.0	33.0	34.0
3	33.34385208869118	34.0	34.0	34.0	33.0	34.0
4	33.37832765691249	34.0	34.0	34.0	34.0	34.0
5	33.35858761381803	34.0	34.0	34.0	33.0	34.0
6	36.509279210398276	38.0	37.0	38.0	34.0	38.0
7	36.5858761381803	38.0	37.0	38.0	34.0	38.0
8	36.14575658580663	38.0	37.0	38.0	34.0	38.0
9	35.97998192812956	38.0	36.0	38.0	32.0	38.0
10-11	36.090220337804965	38.0	36.5	38.0	33.0	38.0
12-13	36.20661013414888	38.0	37.0	38.0	34.0	38.0
14-15	36.17856398137207	38.0	37.0	38.0	34.0	38.0
16-17	36.09793563633836	38.0	36.5	38.0	33.0	38.0
18-19	36.182734413011744	38.0	37.0	38.0	34.0	38.0
20-21	36.204907207896014	38.0	37.0	38.0	34.0	38.0
22-23	35.98175436157642	38.0	36.0	38.0	32.0	38.0
24-25	36.02418850351012	38.0	36.0	38.0	32.5	38.0
26-27	35.845103218183084	38.0	36.0	38.0	31.5	38.0
28-29	35.75710711058595	38.0	36.0	38.0	31.5	38.0
30-31	35.703586571210124	38.0	36.0	38.0	31.0	38.0
32-33	35.695315215124765	38.0	36.0	38.0	31.0	38.0
34-35	35.74056439841523	38.0	36.0	38.0	31.0	38.0
36-37	35.762215889344546	38.0	36.0	38.0	31.0	38.0
38-39	35.91440189059567	38.0	36.0	38.0	32.0	38.0
40-41	35.926218113574755	38.0	36.0	38.0	32.0	38.0
42-43	35.778376311948286	38.0	36.0	38.0	31.0	38.0
44-45	35.80284284423438	38.0	36.0	38.0	31.5	38.0
46-47	35.79405018419406	38.0	36.0	38.0	31.0	38.0
48-49	35.80037533884757	38.0	36.0	38.0	31.5	38.0
50-51	35.812990894557586	38.0	36.0	38.0	32.0	38.0
52-53	35.780913324529095	38.0	36.0	38.0	31.5	38.0
54-55	35.79307708347814	38.0	36.0	38.0	31.5	38.0
56-57	35.77969694863418	38.0	36.0	38.0	31.5	38.0
58-59	35.715541808577186	38.0	36.0	38.0	31.0	38.0
60-61	35.73726280670049	38.0	36.0	38.0	31.0	38.0
62-63	35.600264127337184	38.0	36.0	38.0	31.0	38.0
64-65	35.52961006464169	38.0	36.0	38.0	31.0	38.0
66-67	35.54691735594634	38.0	36.0	38.0	31.0	38.0
68-69	35.47504691735595	38.0	36.0	38.0	31.0	38.0
70-71	35.39292416765136	38.0	36.0	38.0	31.0	38.0
72-73	35.48741919788698	38.0	36.0	38.0	31.0	38.0
74-75	35.52839368874679	38.0	36.0	38.0	31.0	38.0
76	34.32960311392229	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	5.0
24	10.0
25	11.0
26	29.0
27	46.0
28	86.0
29	134.0
30	255.0
31	409.0
32	624.0
33	914.0
34	1502.0
35	2278.0
36	3512.0
37	4570.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.19781747410857	21.762702439702508	11.781469382081045	24.258010704107875
2	22.42997150205046	22.061583373879195	32.84214916243831	22.66629596163203
3	21.018975463960523	24.605546674080767	30.63182039341072	23.743657468547994
4	16.4384513797178	18.203934107180093	37.353166052686454	28.004448460415652
5	14.950997428233823	35.74059915201223	35.108083686661566	14.200319733092376
6	32.50156391186488	36.36616389796344	17.745186626815876	13.387085563355807
7	29.067908528532705	30.305136581636198	22.506429415444497	18.120525474386596
8	26.169458538958786	35.62243692222145	19.329950649892265	18.878153888927503
9	26.315423646347398	18.176131229582264	20.511572947800097	34.996872176270244
10-11	26.676861055119204	25.721137137693752	26.795023284909984	20.806978522277056
12-13	26.808924723708905	23.052060888301938	26.14165566136095	23.997358726628207
14-15	25.234586779731703	23.903524014735524	23.187599916591367	27.674289288941406
16-17	24.69243066657399	27.75074720233544	24.605546674080767	22.9512754570098
18-19	24.303190380204352	26.7845972058108	26.232015013553905	22.680197400430945
20-21	24.205880308611942	26.843678320706193	25.4987141169111	23.451727253770766
22-23	24.105094877319804	27.417112671161465	25.662056022798357	22.815736428720374
24-25	23.816640022242304	27.95231806491972	26.44053659553764	21.79050531730034
26-27	23.879196496837423	27.56307777855008	26.124278862862305	22.43344686175019
28-29	24.011260165427124	27.50399666365469	27.01397094599291	21.47077222492528
30-31	23.319663585181065	27.514422742753876	26.934037672899148	22.231875999165915
32-33	23.646347396955587	28.616111767567944	26.388406200041704	21.34913463543477
34-35	24.605546674080767	26.989643428094805	27.52484882185306	20.879961075971362
36-37	24.400500451796763	27.01049558629318	26.62125529992354	21.967748661986516
38-39	23.611593799958296	26.72551609091541	27.72989504413707	21.93299506498923
40-41	23.747132828247725	25.960936956975043	28.10870925140752	22.18322096336971
42-43	24.21630638771113	26.857579759505107	26.81587544310836	22.1102384096754
44-45	23.204976715090012	27.139083895183152	27.53179954125252	22.124139848474318
46-47	24.17807743101411	25.696809619795648	27.14603461458261	22.97907833460763
48-49	23.941752971432543	26.061722388267185	27.55612705915062	22.44039758114965
50-51	23.420449016473206	25.481337318412457	27.99402238131647	23.104191283797874
52-53	23.17717383749218	25.505664836310558	28.94627093904219	22.37089038715507
54-55	23.347466462778897	25.905331201779386	28.557030652672548	22.190171682769165
56-57	23.56988948356155	25.505664836310558	28.053103496211858	22.871342183916035
58-59	23.11114200319733	26.65948425662056	28.310280113991798	21.91909362619031
60-61	23.34399110307917	28.007923820115384	27.173837492180443	21.474247584625008
62-63	22.940849377910613	28.303329394592343	26.826301522207547	21.929519705289497
64-65	22.76360603322444	29.133940362827556	26.287620768749566	21.814832835198445
66-67	22.555084451240702	28.89761590324599	26.628206019323	21.91909362619031
68-69	22.24577743796483	29.022728852436224	26.68728713421839	22.04420657538055
70-71	22.749704594425523	28.1782164454021	26.7359421700146	22.33613679015778
72-73	22.676722040731214	27.73684576353653	26.409258358240077	23.17717383749218
74-75	22.683672760130673	27.7576979217349	26.645582817821644	22.913046500312785
76	22.72190171682769	27.6013067352471	26.301522207548484	23.37526934037673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	2.0
17	3.0
18	4.0
19	4.5
20	6.5
21	9.0
22	10.0
23	23.0
24	41.0
25	47.0
26	57.0
27	82.5
28	116.0
29	134.0
30	150.0
31	188.0
32	266.5
33	323.0
34	367.5
35	450.0
36	507.0
37	526.0
38	593.0
39	735.5
40	876.5
41	909.0
42	876.0
43	882.5
44	910.0
45	908.5
46	886.0
47	836.0
48	754.0
49	676.5
50	631.0
51	554.0
52	424.5
53	346.5
54	321.0
55	323.0
56	271.0
57	215.0
58	213.0
59	179.5
60	147.0
61	144.0
62	140.0
63	129.5
64	126.0
65	116.0
66	111.5
67	124.0
68	125.5
69	120.0
70	110.5
71	108.0
72	100.0
73	85.5
74	80.5
75	82.0
76	78.0
77	67.5
78	55.5
79	50.0
80	39.5
81	26.5
82	18.0
83	12.0
84	11.0
85	9.0
86	6.0
87	4.0
88	2.5
89	0.5
90	1.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	14387.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.99645513310628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.08036564463912	56.99589907555433
2	14.854313464102075	21.686244526308474
3	3.9706722529042087	8.695349968721763
4	1.6377832793753573	4.782094946826996
5	0.5522757569986669	2.0157086258427745
6	0.3523138449819082	1.5430597066796412
7	0.27613787849933347	1.4109960380899422
8	0.047609979051609214	0.27802877597831377
9	0.057131974861931056	0.37533884757072355
>10	0.16187392877547135	1.466601793285605
>50	0.0	0.0
>100	0.009521995810321844	0.7506776951414471
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	108	0.7506776951414471	TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	28	0.19462014318481963	TruSeq Adapter, Index 5 (96% over 26bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.006950719399457844	0.0
20	0.0	0.0	0.0	0.013901438798915687	0.0
21	0.0	0.0	0.0	0.013901438798915687	0.0
22	0.0	0.0	0.0	0.013901438798915687	0.0
23	0.0	0.0	0.0	0.013901438798915687	0.0
24	0.0	0.0	0.0	0.013901438798915687	0.0
25	0.0	0.0	0.0	0.013901438798915687	0.0
26	0.0	0.0	0.0	0.013901438798915687	0.0
27	0.0	0.0	0.0	0.013901438798915687	0.0
28	0.0	0.0	0.0	0.013901438798915687	0.0
29	0.0	0.0	0.0	0.04865503579620491	0.0
30	0.0	0.0	0.0	0.04865503579620491	0.0
31	0.0	0.0	0.0	0.05560575519566275	0.0
32	0.0	0.0	0.0	0.06255647459512059	0.0
33	0.0	0.0	0.0	0.06950719399457844	0.0
34	0.0	0.0	0.0	0.08340863279349413	0.0
35	0.0	0.0	0.0	0.09035935219295196	0.0
36	0.0	0.0	0.0	0.13901438798915688	0.0
37	0.0	0.0	0.0	0.21547230138319315	0.0
38	0.0	0.0	0.0	0.2849794953777716	0.0
39	0.0	0.0	0.0	0.3336345311739765	0.0
40	0.0	0.0	0.0	0.41704316396747065	0.0
41	0.0	0.0	0.0	0.5004517967609647	0.0
42	0.0	0.0	0.0	0.5977618683533745	0.0
43	0.0	0.0	0.0	0.7854312921387364	0.0
44	0.0	0.0	0.0	0.9591992771251825	0.0
45	0.0	0.0	0.0	1.1746715785083757	0.0
46	0.0	0.0	0.0	1.3901438798915688	0.0
47	0.0	0.0	0.0	1.598665461875304	0.0
48	0.0	0.0	0.0	1.7654827274622924	0.0
49	0.0	0.0	0.0	1.9879057482449434	0.0
50	0.0	0.0	0.0	2.2033780496281365	0.0
51	0.0	0.0	0.0	2.48140682560645	0.0
52	0.0	0.0	0.0	2.724682004587475	0.0
53	0.0	0.0	0.0	2.995760061166331	0.0
54	0.0	0.0	0.0	3.197330923750608	0.0
55	0.0	0.0	0.0	3.3641481893375964	0.0
56	0.0	0.0	0.0	3.530965454924585	0.0
57	0.0	0.0	0.0	3.7533884757072355	0.0
58	0.0	0.0	0.0	4.073121568082296	0.0
59	0.0	0.0	0.0	4.2955445888649475	0.0
60	0.0	0.0	0.0	4.4832140126503095	0.0
61	0.0	0.0	0.0	4.782094946826996	0.0
62	0.0	0.0	0.0	5.046222284006395	0.0
63	0.0	0.0	0.0	5.372906095780913	0.0
64	0.0	0.0	0.0	5.70654062695489	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTCG	15	0.0021802788	70.0	36
TTAGGCT	15	0.0021802788	70.0	3
AAAAATT	15	0.0021802788	70.0	67
CCGTCTT	25	2.3120898E-4	55.999996	47
CTGCTTG	25	2.3120898E-4	55.999996	54
CGTCTTC	25	2.3120898E-4	55.999996	48
ATCTCGT	20	0.0068136547	52.5	37
TGATCTC	20	0.0068136547	52.5	35
GTGATCT	20	0.0068136547	52.5	34
AAGTGAT	20	0.0068136547	52.5	32
TCTGCTT	35	1.9852818E-5	50.000004	53
TGCCGTC	30	5.686422E-4	46.666664	45
TATGCCG	30	5.686422E-4	46.666664	43
TTCTGCT	30	5.686422E-4	46.666664	52
ATGCCGT	30	5.686422E-4	46.666664	44
CGTATGC	30	5.686422E-4	46.666664	41
GCTTGAA	30	5.686422E-4	46.666664	56
TCGTATG	30	5.686422E-4	46.666664	40
GCCGTCT	30	5.686422E-4	46.666664	46
CGAGACA	30	5.686422E-4	46.666664	23
>>END_MODULE