##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779694_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77886
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.15416120997355	34.0	31.0	34.0	21.0	34.0
2	28.517243150245232	34.0	23.0	34.0	12.0	34.0
3	27.576547774953138	33.0	23.0	34.0	11.0	34.0
4	30.14091107516113	34.0	31.0	34.0	21.0	34.0
5	29.613640448861155	34.0	31.0	34.0	11.0	34.0
6	32.08322419947102	37.0	31.0	38.0	12.0	38.0
7	33.20080630665331	37.0	34.0	38.0	21.0	38.0
8	32.3929461007113	37.0	31.0	38.0	12.0	38.0
9	33.75476979174691	38.0	34.0	38.0	21.0	38.0
10-11	33.57649641784146	38.0	34.0	38.0	21.0	38.0
12-13	32.47593919317978	37.5	30.5	38.0	16.0	38.0
14-15	32.638009398351436	37.0	32.5	38.0	16.0	38.0
16-17	32.28186066815602	37.5	30.0	38.0	16.0	38.0
18-19	32.83532342141078	37.0	32.5	38.0	16.0	38.0
20-21	31.8480022083558	36.5	29.5	38.0	16.0	38.0
22-23	31.780936240145852	36.5	28.5	38.0	16.0	38.0
24-25	33.135370926739085	37.0	32.5	38.0	16.5	38.0
26-27	31.55330868191973	37.0	28.0	38.0	11.0	38.0
28-29	33.76852707803713	37.5	34.5	38.0	21.0	38.0
30-31	34.17679043730581	38.0	36.0	38.0	21.0	38.0
32-33	34.27271910227769	38.0	36.0	38.0	22.0	38.0
34-35	34.297749274580795	38.0	36.0	38.0	21.5	38.0
36-37	32.20162802044013	37.0	30.0	38.0	16.0	38.0
38-39	30.385878078216884	35.5	25.0	38.0	11.0	38.0
40-41	31.688698867575688	36.5	28.5	38.0	11.0	38.0
42-43	32.72940579821791	37.0	30.5	38.0	16.0	38.0
44-45	34.287734637803965	38.0	36.0	38.0	22.5	38.0
46-47	32.846352361143204	37.5	31.0	38.0	17.0	38.0
48-49	32.267140436021876	37.0	30.0	38.0	16.0	38.0
50-51	30.085952546028814	35.5	23.5	38.0	11.0	38.0
52-53	33.5023303289423	37.0	34.5	38.0	21.0	38.0
54-55	32.58334617261126	37.5	30.5	38.0	16.0	38.0
56-57	32.9737308373777	37.5	31.5	38.0	16.0	38.0
58-59	34.07405695503685	38.0	35.0	38.0	21.0	38.0
60-61	34.46332460262435	38.0	36.5	38.0	23.0	38.0
62-63	34.346345941504254	38.0	36.5	38.0	22.0	38.0
64-65	34.064466014431346	38.0	35.5	38.0	21.5	38.0
66-67	32.11692088436946	37.0	29.0	38.0	11.0	38.0
68-69	32.14493618878874	37.0	29.0	38.0	11.0	38.0
70-71	31.589322856482553	37.0	28.0	38.0	11.0	38.0
72-73	30.519785327273194	36.0	24.5	38.0	11.0	38.0
74-75	32.63624399763758	37.0	30.5	38.0	16.0	38.0
76	33.32348560716945	37.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	11.0
13	108.0
14	326.0
15	558.0
16	896.0
17	1331.0
18	1560.0
19	1490.0
20	1297.0
21	1044.0
22	814.0
23	693.0
24	734.0
25	695.0
26	795.0
27	1010.0
28	1199.0
29	1508.0
30	2054.0
31	2768.0
32	3884.0
33	5988.0
34	8763.0
35	13101.0
36	16068.0
37	9190.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.43787073414991	20.729014200241377	14.295252035025548	28.53786303058316
2	20.017718203528233	19.093290193359525	39.07377449092263	21.81521711218961
3	21.03202115913001	19.443802480548495	35.472357034640375	24.051819325681123
4	14.512235831856815	19.681329122050176	32.94943892355494	32.85699612253807
5	15.102842616131268	30.07600852528054	38.10184115245359	16.719307706134607
6	27.728988521685537	33.216495904270346	17.054412859820765	22.000102714223353
7	24.674524304753103	28.379939912179342	21.625195798988265	25.320339984079293
8	21.662429704953393	33.0316103022366	19.980484297563105	25.3254756952469
9	22.471304213851013	17.281668078987238	21.63418329353157	38.61284441363018
10-11	23.991474719461777	25.279896258634416	27.253935238682175	23.474693783221632
12-13	23.621703515394294	21.52633335901189	29.28061525819788	25.57134786739594
14-15	22.70369514418509	24.278432580951648	24.532008319852093	28.48586395501117
16-17	23.383534909996662	25.13288652646175	26.59014457026937	24.893433993272218
18-19	21.258634414400536	25.114269573479188	28.408828287497112	25.218267724623168
20-21	23.402793826875175	24.353542356777854	27.483758313432453	24.759905502914517
22-23	23.74367665562489	23.415633104794185	28.152684693012866	24.68800554656806
24-25	22.36538017101918	26.088770767532033	24.94671699663611	26.599132064812675
26-27	22.499550625272835	24.705980535654675	28.125722209382943	24.668746629689547
28-29	22.446267621908945	25.874354826284566	26.55483655599209	25.124540995814392
30-31	22.607400559792517	26.065018103381867	26.367383098374546	24.96019823845107
32-33	22.41352746321547	25.588680892586602	26.74100608581773	25.256785558380194
34-35	22.33520786790951	25.65416120997355	26.700562360372853	25.310068561744085
36-37	22.772385281051793	23.956808669080452	30.26025216341833	23.010553886449426
38-39	22.96497444983694	22.686362118994428	32.868551472665175	21.480111958503453
40-41	22.66004159926046	24.410677143517447	25.807590581105718	27.121690676116376
42-43	21.169401432863417	24.68543769098426	27.13388799013943	27.01127288601289
44-45	21.412063785532702	24.648203785019128	28.0705133143312	25.869219115116966
46-47	22.719744241583854	24.00752381686054	29.001360963459415	24.27137097809619
48-49	22.93544411062322	24.128213029299232	29.20550548237167	23.73083737770588
50-51	23.776416814318363	22.45653904424415	31.56472279998973	22.202321341447757
52-53	20.759828467247	25.15728115450787	28.507690727473488	25.57519965077164
54-55	22.594561281873506	24.259173664073135	28.21046144364841	24.93580361040495
56-57	22.33328197622166	24.43828159104332	27.70202603805562	25.526410394679406
58-59	20.580720540276815	25.480188994170966	28.337570295046604	25.60152017050561
60-61	20.80540790385949	25.02054284467042	27.791900983488688	26.38214826798141
62-63	20.590991962612023	25.751739722158025	27.705877821431322	25.951390493798627
64-65	20.843283773720568	25.692679043730582	27.36114320930591	26.102893973242946
66-67	19.782117453714402	25.208638266183907	24.756695683434764	30.252548596666923
68-69	20.259780284692482	25.44575497441363	25.161319319152213	29.13314542174167
70-71	19.969570911331946	25.52320057519965	24.674524304753103	29.8327042087153
72-73	19.63446575764579	24.888298282104614	23.86243997637573	31.61479598387387
74-75	21.508358369925276	26.42002413784249	25.737616516447115	26.33400097578512
76	21.765143928305474	26.251187633207508	25.50137380273733	26.482294635749685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	3.0
14	5.5
15	13.0
16	24.0
17	28.0
18	45.0
19	85.5
20	140.5
21	172.0
22	213.5
23	309.0
24	432.5
25	502.0
26	602.5
27	745.0
28	908.5
29	1030.0
30	1197.5
31	1505.5
32	1831.5
33	2017.0
34	2093.0
35	2371.5
36	2686.0
37	2798.0
38	2886.0
39	3010.5
40	3132.0
41	3247.5
42	3278.0
43	3267.5
44	3289.0
45	3275.0
46	3229.0
47	3183.0
48	3051.5
49	2917.5
50	2869.0
51	2781.5
52	2614.0
53	2465.5
54	2397.0
55	2338.5
56	2198.5
57	2084.0
58	2051.0
59	1898.0
60	1710.0
61	1647.5
62	1620.0
63	1528.0
64	1384.0
65	1274.0
66	1175.0
67	1134.0
68	1084.5
69	966.5
70	865.5
71	833.0
72	772.5
73	639.5
74	504.5
75	442.0
76	393.5
77	319.0
78	254.5
79	216.0
80	177.5
81	118.5
82	77.5
83	57.0
84	53.0
85	37.0
86	19.5
87	14.0
88	13.5
89	10.0
90	4.0
91	1.0
92	1.0
93	1.5
94	1.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.016049097398762292
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	77886.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.10620650694605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.2319299577286	96.37162006008782
2	1.625420423760977	3.189276635082043
3	0.12694507335331298	0.37362298744318617
4	0.011778408867833165	0.04622140050843541
5	0.003926136289277722	0.01925891687851475
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.002567855583801967	0.0
10	0.0	0.0	0.0	0.002567855583801967	0.0
11	0.0	0.0	0.0	0.0038517833757029506	0.0
12	0.0	0.0	0.0	0.0038517833757029506	0.0
13	0.0	0.0	0.0	0.0038517833757029506	0.0
14	0.0	0.0	0.0	0.005135711167603934	0.0
15	0.0	0.0	0.0	0.005135711167603934	0.0
16	0.0	0.0	0.0	0.007703566751405901	0.0
17	0.0	0.0	0.0	0.007703566751405901	0.0
18	0.0	0.0	0.0	0.008987494543306884	0.0
19	0.0	0.0	0.0	0.015407133502811803	0.0
20	0.0	0.0	0.0	0.016691061294712786	0.0
21	0.0	0.0	0.0	0.016691061294712786	0.0
22	0.0	0.0	0.0	0.02953033921372262	0.0
23	0.0	0.0	0.0	0.030814267005623605	0.0
24	0.0	0.0	0.0	0.03466605038132656	0.0
25	0.0	0.0	0.0	0.03723390596512852	0.0
26	0.0	0.0	0.0	0.03723390596512852	0.0
27	0.0	0.0	0.0	0.04622140050843541	0.0
28	0.0	0.0	0.0	0.060344606219346224	0.0
29	0.0	0.0	0.0	0.06676424517885114	0.0
30	0.0	0.0	0.0	0.08602316205736589	0.0
31	0.0	0.0	0.0	0.09501065660067277	0.0
32	0.0	0.0	0.0	0.10528207893588065	0.0
33	0.0	0.0	0.0	0.13352849035770228	0.0
34	0.0	0.0	0.0	0.16434275736332588	0.0
35	0.0	0.0	0.0	0.23752664150168196	0.0
36	0.0	0.0	0.0	0.2875998253858203	0.0
37	0.0	0.0	0.0	0.4275479547030275	0.0
38	0.0	0.0	0.0	0.5700639396040367	0.0
39	0.0	0.0	0.0	0.703592429961739	0.0
40	0.0	0.0	0.0	0.8679351873250648	0.0
41	0.0	0.0	0.0	1.0733636340292223	0.0
42	0.0	0.0	0.0	1.3327170479932209	0.0
43	0.0	0.0	0.0	1.5920704619572195	0.0
44	0.0	0.0	0.0	1.8848059985106438	0.0
45	0.0	0.0	0.0	2.110777289885217	0.0
46	0.0	0.0	0.0	2.36242713709781	0.0
47	0.0	0.0	0.0	2.6063734175589963	0.0
48	0.0	0.0	0.0	2.7565929692114115	0.0
49	0.0	0.0	0.0	2.9350589322856484	0.0
50	0.0	0.0	0.0	3.1713016459954293	0.0
51	0.0	0.0	0.0	3.4537657602136456	0.0
52	0.0	0.0	0.0	3.736229874431862	0.0
53	0.0	0.0	0.0	4.16506175692679	0.0
54	0.0	0.0	0.0	4.49117941606964	0.0
55	0.0	0.0	0.0	4.753100685617441	0.0
56	0.0	0.0	0.0	5.024009449708548	0.0
57	0.0	0.0	0.0	5.220450401869399	0.0
58	0.0	0.0	0.0	5.415607426238348	0.0
59	0.0	0.0	0.0	5.60049302827209	0.0
60	0.0	0.0	0.0	5.731453663045991	0.0
61	0.0	0.0	0.0	5.898364275993118	0.0
62	0.0	0.0	0.0	6.01776956063991	0.0
63	0.0	0.0012839277919009834	0.0	6.171840895668027	0.0
64	0.0	0.0012839277919009834	0.0	6.355442569909869	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGTTG	15	0.0022171	70.0	35
GACGCTC	15	0.0022171	70.0	14
CTAGCAC	15	0.0022171	70.0	3
ACGCTTC	25	2.377169E-4	56.000004	15
TATGCCG	20	0.0069278385	52.5	68
CGGTGGT	60	8.185452E-11	52.5	46
TGTGTCG	20	0.0069278385	52.5	35
GTGTCGA	35	2.0694217E-5	50.0	36
TCTCGGT	70	7.2759576E-12	50.0	43
CCGACGC	35	2.0694217E-5	50.0	21
TGGTCGC	35	2.0694217E-5	50.0	49
GTGGTCG	50	6.101982E-8	49.000004	48
CTCGGTG	80	0.0	48.125	44
CGCCGTA	30	5.8455067E-4	46.666664	53
TCGGTGG	75	7.385097E-10	42.000004	45
GGTGGTT	60	2.5583722E-7	40.833332	47
GCCGTAT	35	0.0012485727	40.0	54
AGATCTC	45	9.123092E-5	38.888885	40
ATCTCGG	65	4.792746E-7	37.692307	42
GTGGTTG	75	3.470086E-8	37.333336	48
>>END_MODULE