FastQCFastQC Report
Wed 25 May 2016
SRR1779694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77886
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT48216.189815884754641No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG6160.7908995198110058No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5220.6702103073723134RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC3620.4647818606681561No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2770.3556479983565724No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2650.34024086485376065No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2150.27604447525871145RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT980.12582492360629638No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG900.11555350127108852No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT890.11426957347918754RNA PCR Primer, Index 48 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC890.11426957347918754No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCTA150.002217170.044
GTGTTGC200.006927838552.511
ACAAAGT200.006927838552.512
AAACCTC200.006927838552.567
TTTATAC552.239176E-950.909093
TCTCGTA8900.044.438238
CTCGTAT9500.044.2105339
TCGTATG9600.044.11458240
TATGCCG9450.044.07407443
TGCCGTC9500.043.84210645
CGTCTTC9600.043.7548
AATCTCG8900.043.65168436
CGTATGC9550.043.61256441
ATGCCGT9550.043.61256444
TCAATCT8750.043.634
ATCTCGT9100.043.46153637
CGTCAAT8800.043.35227232
CTTCTGC9700.043.2989751
GTCAATC8750.043.233
GTATGCC9650.043.1606242