##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779694_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77886
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.28641861181727	34.0	34.0	34.0	33.0	34.0
2	33.437524073646095	34.0	34.0	34.0	34.0	34.0
3	33.486801222299256	34.0	34.0	34.0	34.0	34.0
4	33.52295662891919	34.0	34.0	34.0	34.0	34.0
5	33.500333821225894	34.0	34.0	34.0	34.0	34.0
6	36.885037105513184	38.0	37.0	38.0	35.0	38.0
7	36.91482423028529	38.0	37.0	38.0	35.0	38.0
8	36.65377603163598	38.0	37.0	38.0	34.0	38.0
9	36.69347507896156	38.0	37.0	38.0	34.0	38.0
10-11	36.583063708497036	38.0	37.0	38.0	34.0	38.0
12-13	36.610931361220246	38.0	37.0	38.0	34.0	38.0
14-15	36.63485093598336	38.0	37.0	38.0	34.0	38.0
16-17	36.52497881519143	38.0	37.0	38.0	34.0	38.0
18-19	36.56844619058624	38.0	37.0	38.0	34.0	38.0
20-21	36.63375959736024	38.0	37.0	38.0	34.0	38.0
22-23	36.486852579410936	38.0	37.0	38.0	34.0	38.0
24-25	36.47581080040059	38.0	37.0	38.0	34.0	38.0
26-27	36.33916878514752	38.0	37.0	38.0	34.0	38.0
28-29	36.24659759135146	38.0	37.0	38.0	34.0	38.0
30-31	36.21361990601649	38.0	37.0	38.0	34.0	38.0
32-33	36.13872197827594	38.0	37.0	38.0	33.5	38.0
34-35	36.05282720899777	38.0	37.0	38.0	33.0	38.0
36-37	36.09656420922887	38.0	37.0	38.0	33.0	38.0
38-39	35.81033176694143	38.0	37.0	38.0	31.5	38.0
40-41	36.11443648409214	38.0	37.0	38.0	34.0	38.0
42-43	36.24024214878155	38.0	37.0	38.0	34.0	38.0
44-45	36.227678915337805	38.0	37.0	38.0	34.0	38.0
46-47	36.251476516960686	38.0	37.0	38.0	34.0	38.0
48-49	36.23540816064504	38.0	37.0	38.0	34.0	38.0
50-51	36.14734355339856	38.0	37.0	38.0	34.0	38.0
52-53	36.17121177105	38.0	37.0	38.0	34.0	38.0
54-55	36.141681431836275	38.0	37.0	38.0	34.0	38.0
56-57	36.155015021955165	38.0	37.0	38.0	34.0	38.0
58-59	36.07987314793416	38.0	37.0	38.0	34.0	38.0
60-61	36.117164830649926	38.0	37.0	38.0	34.0	38.0
62-63	36.1118750481473	38.0	37.0	38.0	34.0	38.0
64-65	36.12463087075983	38.0	37.0	38.0	34.0	38.0
66-67	36.10370284775185	38.0	37.0	38.0	33.5	38.0
68-69	35.88384305266672	38.0	37.0	38.0	31.5	38.0
70-71	35.23513211616979	38.0	36.5	38.0	28.0	38.0
72-73	34.28415247926456	38.0	36.0	38.0	22.5	38.0
74-75	34.19231312430989	38.0	36.0	38.0	22.0	38.0
76	33.45037619084302	37.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	4.0
23	20.0
24	49.0
25	90.0
26	151.0
27	267.0
28	450.0
29	730.0
30	1222.0
31	1822.0
32	2899.0
33	4377.0
34	6700.0
35	10617.0
36	14774.0
37	33713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.092494158128545	19.655650566212156	13.21161697866112	31.04023829699818
2	17.969853375446164	20.370798346301004	39.227845825950745	22.431502452302084
3	18.740210050586757	22.068150887194104	34.921552011914855	24.27008705030429
4	14.157871761292146	23.12097167655291	33.818658038671906	28.90249852348304
5	14.381275197082916	32.91477287317362	37.64989856970444	15.054053360039033
6	27.644249287420074	39.47821174537144	17.484528670107593	15.39301029710089
7	24.62445112086896	29.476414246462777	20.659682099478726	25.23945253318953
8	21.523765503428088	39.47435996199574	19.669773771923065	19.33210076265311
9	21.703515394294225	17.381814446755513	19.848239734997303	41.06643042395295
10-11	23.554939270215446	27.2160593688211	27.808592044783403	21.42040931618006
12-13	22.939937857894872	20.81054361502709	28.535937138895306	27.713581388182728
14-15	21.882623321264415	22.29861592584033	22.212592763782965	33.60616798911229
16-17	25.59702642323396	28.34078011452636	22.132989240685102	23.929204221554578
18-19	25.738900444239015	25.27219269188301	23.217266260945486	25.77164060293249
20-21	22.134915132372956	23.87977300156639	23.044577972934803	30.94073389312585
22-23	22.1304213851013	27.8708625426906	23.069614564876872	26.929101507331225
24-25	26.12985645687286	28.22458464935932	22.635005007318387	23.010553886449426
26-27	25.57134786739594	28.49677734124233	24.378578948719923	21.55329584264181
28-29	21.381249518527078	24.46267621908944	28.76961199702129	25.386462265362198
30-31	21.52761728680379	23.39380633233187	33.040597796779906	22.03797858408443
32-33	24.803559047839148	23.97927740543872	25.74211026371877	25.475053283003362
34-35	22.14518655470816	24.004313997380788	28.284287291682713	25.566212156228335
36-37	21.058341678863982	31.900469917571833	24.771460853041624	22.269727550522557
38-39	20.964614950055207	25.019258916878517	28.63800939835144	25.37811673471484
40-41	21.12061217677118	23.402793826875175	29.13617338160902	26.340420614744627
42-43	25.304932850576485	23.347584931823434	28.794648588963355	22.552833628636726
44-45	21.689392188583316	27.293737000231104	29.294096500012838	21.722774311172742
46-47	24.89921166833577	23.555581234111393	25.799245050458364	25.745962047094473
48-49	24.595562745551188	22.973961944380246	25.099504403872324	27.330970906196235
50-51	21.066687209511336	23.151785943558533	29.640757003826106	26.14076984310402
52-53	20.20645558893768	23.00541817528182	33.65238938962073	23.135736846159773
54-55	20.61217677117839	22.721028169375753	30.480445779729347	26.18634927971651
56-57	24.179570140975272	22.61253627096012	26.899571168117504	26.3083224199471
58-59	20.454253652774568	23.48303931386899	33.02968441054875	23.033022622807696
60-61	23.977993477646816	28.376088128803634	25.920576226793006	21.72534216675654
62-63	19.929769149783017	34.07865341653186	24.116657679172125	21.874919754513005
64-65	19.62098451583083	34.26803276583725	23.845106951185066	22.265875767146856
66-67	19.73974783658167	34.742444084944665	23.27632693937293	22.24148113910074
68-69	19.62034255193488	34.237860462727575	23.867575687543333	22.27422129779421
70-71	19.631897902061986	32.97575944328891	24.121793390339725	23.270549264309377
72-73	19.78853709267391	30.584443930873327	25.16434275736333	24.46267621908944
74-75	20.2321341447757	29.294096500012838	25.568780011812137	24.90498934339933
76	20.44398223043936	29.602239170069076	24.85940990678684	25.094368692704723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	3.0
11	5.0
12	4.0
13	3.0
14	6.5
15	20.0
16	45.5
17	62.0
18	72.0
19	118.0
20	206.0
21	258.0
22	306.0
23	419.5
24	570.0
25	655.0
26	732.5
27	923.5
28	1129.0
29	1221.0
30	1353.0
31	1666.0
32	1943.5
33	2040.0
34	2076.0
35	2282.5
36	2516.0
37	2579.0
38	2708.5
39	3791.0
40	5159.0
41	5183.5
42	4793.0
43	4315.5
44	3468.5
45	2868.0
46	2637.0
47	2539.0
48	2340.0
49	2178.0
50	2117.0
51	2050.5
52	1929.0
53	1805.0
54	1736.0
55	1707.5
56	1714.5
57	1662.0
58	1574.0
59	1491.0
60	1454.0
61	1444.5
62	1389.0
63	1305.0
64	1211.0
65	1125.0
66	1061.0
67	1073.0
68	976.0
69	874.0
70	865.5
71	862.0
72	786.5
73	713.5
74	631.0
75	546.0
76	513.5
77	422.5
78	327.5
79	291.0
80	275.0
81	209.0
82	136.5
83	114.0
84	92.0
85	64.5
86	46.0
87	33.0
88	21.5
89	9.5
90	7.0
91	3.0
92	1.0
93	1.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	77886.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.03461469326966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.98287684966115	45.45361168887862
2	20.884629265749687	26.746783760881286
3	5.9790672494686605	11.486018026346198
4	1.5338653406584593	3.9288190432170094
5	0.37494486104984565	1.2004724854274196
6	0.09423747844568313	0.36206763731607733
7	0.030075790993303122	0.13481241814960326
8	0.022055580061755623	0.11298564568728656
9	0.010025263664434375	0.05777675063554426
>10	0.058146529253719374	0.6445317515342938
>50	0.016040421863094997	0.7844798808515009
>100	0.008020210931547499	1.4367151991372005
>500	0.004010105465773749	1.4611098271833192
>1k	0.0020050527328868747	6.189815884754641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	4821	6.189815884754641	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	616	0.7908995198110058	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	522	0.6702103073723134	RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	362	0.4647818606681561	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	277	0.3556479983565724	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	265	0.34024086485376065	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	215	0.27604447525871145	RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	98	0.12582492360629638	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	90	0.11555350127108852	No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT	89	0.11426957347918754	RNA PCR Primer, Index 48 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	89	0.11426957347918754	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0012839277919009834	0.0
5	0.0	0.0	0.0	0.0012839277919009834	0.0
6	0.0	0.0	0.0	0.0012839277919009834	0.0
7	0.0	0.0	0.0	0.002567855583801967	0.0
8	0.0	0.0	0.0	0.002567855583801967	0.0
9	0.0	0.0	0.0	0.0064196389595049174	0.0
10	0.0	0.0	0.0	0.0064196389595049174	0.0
11	0.0	0.0	0.0	0.007703566751405901	0.0
12	0.0	0.0	0.0	0.007703566751405901	0.0
13	0.0	0.0	0.0	0.007703566751405901	0.0
14	0.0	0.0	0.0	0.010271422335207867	0.0
15	0.0	0.0	0.0	0.010271422335207867	0.0
16	0.0	0.0	0.0	0.012839277919009835	0.0
17	0.0	0.0	0.0	0.012839277919009835	0.0
18	0.0	0.0	0.0	0.015407133502811803	0.0
19	0.0	0.0	0.0	0.02567855583801967	0.0
20	0.0	0.0	0.0	0.02567855583801967	0.0
21	0.0	0.0	0.0	0.02567855583801967	0.0
22	0.0	0.0	0.0	0.026962483629920653	0.0
23	0.0	0.0	0.0	0.028246411421821636	0.0
24	0.0	0.0	0.0	0.03594997817322754	0.0
25	0.0	0.0	0.0	0.042369617132732455	0.0
26	0.0	0.0	0.0	0.042369617132732455	0.0
27	0.0	0.0	0.0	0.052641039467940326	0.0
28	0.0	0.0	0.0	0.06804817297075212	0.0
29	0.0	0.0	0.0	0.08473923426546491	0.0
30	0.0	0.0	0.0	0.10528207893588065	0.0
31	0.0	0.0	0.0	0.12967670698199935	0.0
32	0.0	0.0	0.0	0.15278740723621703	0.0
33	0.0	0.0	0.0	0.20414451891225638	0.0
34	0.0	0.0	0.0	0.2580694861720977	0.0
35	0.0	0.0	0.0	0.3646354928998793	0.0
36	0.0	0.0	0.0	0.5110032611765915	0.0
37	0.0	0.0	0.0	0.7048763577536399	0.0
38	0.0	0.0	0.0	0.937267288087718	0.0
39	0.0	0.0	0.0	1.1118814677862516	0.0
40	0.0	0.0	0.0012839277919009834	1.3417045425365277	0.0
41	0.0	0.0	0.0012839277919009834	1.5895026063734174	0.0
42	0.0	0.0	0.0012839277919009834	1.9181881211000693	0.0
43	0.0	0.0	0.0012839277919009834	2.2353182856996123	0.0
44	0.0	0.0	0.0012839277919009834	2.629484117813214	0.0
45	0.0	0.0	0.0012839277919009834	3.031353516678222	0.0
46	0.0	0.0	0.0012839277919009834	3.410112215289012	0.0
47	0.0	0.0	0.0012839277919009834	3.737513802223763	0.0
48	0.0	0.0	0.0012839277919009834	4.039236833320494	0.0
49	0.0	0.0	0.0012839277919009834	4.369206275839047	0.0
50	0.0	0.0	0.0012839277919009834	4.646534678889659	0.0
51	0.0	0.0	0.0012839277919009834	4.972652338032509	0.0
52	0.0	0.0	0.0012839277919009834	5.344991397683795	0.0
53	0.0	0.0	0.0012839277919009834	5.787946485889633	0.0
54	0.0	0.0	0.0012839277919009834	6.155149834373315	0.0
55	0.0	0.0	0.0012839277919009834	6.548031738695016	0.0
56	0.0	0.0	0.0012839277919009834	6.925506509513905	0.0
57	0.0	0.0	0.0012839277919009834	7.227229540610636	0.0
58	0.0	0.0	0.0012839277919009834	7.546927560793981	0.0
59	0.0	0.0	0.0012839277919009834	7.824255963844593	0.0
60	0.0	0.0	0.0012839277919009834	8.099016511311405	0.0
61	0.0	0.0	0.0012839277919009834	8.435405592789461	0.0
62	0.0	0.0	0.0012839277919009834	8.779498241018926	0.0
63	0.0	0.0	0.0012839277919009834	9.131294455999795	0.0
64	0.0	0.0	0.0012839277919009834	9.506201371234882	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGCTA	15	0.0022171	70.0	44
GTGTTGC	20	0.0069278385	52.5	11
ACAAAGT	20	0.0069278385	52.5	12
AAACCTC	20	0.0069278385	52.5	67
TTTATAC	55	2.239176E-9	50.90909	3
TCTCGTA	890	0.0	44.4382	38
CTCGTAT	950	0.0	44.21053	39
TCGTATG	960	0.0	44.114582	40
TATGCCG	945	0.0	44.074074	43
TGCCGTC	950	0.0	43.842106	45
CGTCTTC	960	0.0	43.75	48
AATCTCG	890	0.0	43.651684	36
CGTATGC	955	0.0	43.612564	41
ATGCCGT	955	0.0	43.612564	44
TCAATCT	875	0.0	43.6	34
ATCTCGT	910	0.0	43.461536	37
CGTCAAT	880	0.0	43.352272	32
CTTCTGC	970	0.0	43.29897	51
GTCAATC	875	0.0	43.2	33
GTATGCC	965	0.0	43.16062	42
>>END_MODULE