FastQCFastQC Report
Wed 25 May 2016
SRR1779693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32484
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA150.00220541970.056
CGGCATT150.00220541970.065
ATTACGG150.00220541970.028
TACCTGA150.00220541970.058
TAGATCT150.00220541970.039
CATCGGC150.00220541970.062
ACCCCAA150.00220541970.040
GTCGCCG200.00689161852.49999651
TTATGAT200.00689161852.49999617
GGTCCTT200.00689161852.49999642
TACGGAT200.00689161852.49999630
AACATCG200.00689161852.49999660
TATTACG200.00689161852.49999627
CTTCTAG200.00689161852.4999961
GGCATTA200.00689161852.49999666
ATCGGCA200.00689161852.49999663
GCTCGGA200.00689161852.49999611
AAAATCC200.00689161852.4999968
CTTTGAC305.794888E-446.6666649
CCCCTCT501.6742019E-435.0000047
Produced by FastQC (version 0.11.5)