##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779693_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32484
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.836842753355498	34.0	32.0	34.0	21.0	34.0
2	28.65444526536141	34.0	23.0	34.0	12.0	34.0
3	27.8198805565817	34.0	23.0	34.0	11.0	34.0
4	30.7211550301687	34.0	32.0	34.0	23.0	34.0
5	29.918113532816157	34.0	31.0	34.0	11.0	34.0
6	32.7918667651767	37.0	33.0	38.0	12.0	38.0
7	33.669067848787094	37.0	34.0	38.0	21.0	38.0
8	32.9896256618643	37.0	34.0	38.0	12.0	38.0
9	34.486855067109964	38.0	35.0	38.0	26.0	38.0
10-11	34.282400566432706	38.0	35.0	38.0	23.5	38.0
12-13	33.02081024504371	37.5	31.5	38.0	18.5	38.0
14-15	33.31156877231868	37.0	33.0	38.0	21.0	38.0
16-17	32.83659647826622	37.5	31.5	38.0	18.5	38.0
18-19	33.40338320403891	37.0	33.5	38.0	22.0	38.0
20-21	32.33839736485655	37.0	30.0	38.0	17.0	38.0
22-23	32.269809752493536	37.0	29.0	38.0	17.0	38.0
24-25	33.91057135820712	37.5	34.0	38.0	23.0	38.0
26-27	32.09626277552026	37.0	30.0	38.0	16.0	38.0
28-29	34.61824282723802	38.0	35.5	38.0	25.5	38.0
30-31	35.05699729097402	38.0	37.0	38.0	26.5	38.0
32-33	35.17784139884251	38.0	37.0	38.0	27.0	38.0
34-35	35.21512129048147	38.0	37.0	38.0	27.0	38.0
36-37	32.835165004309815	37.0	30.5	38.0	17.0	38.0
38-39	30.5894132495998	36.0	25.5	38.0	11.0	38.0
40-41	32.350557197389485	36.5	30.5	38.0	16.0	38.0
42-43	33.54711550301687	37.5	33.0	38.0	18.5	38.0
44-45	35.208733530353406	38.0	37.0	38.0	27.0	38.0
46-47	33.55844415712351	37.5	32.0	38.0	19.0	38.0
48-49	32.80165619997537	37.0	30.5	38.0	18.0	38.0
50-51	30.432643763083362	36.0	24.5	38.0	11.0	38.0
52-53	34.36291712843246	37.5	35.0	38.0	25.0	38.0
54-55	33.30156384681689	37.5	33.0	38.0	18.5	38.0
56-57	33.78587920206871	37.5	34.0	38.0	19.0	38.0
58-59	35.0076499199606	38.0	36.5	38.0	26.5	38.0
60-61	35.42419344908262	38.0	37.0	38.0	29.5	38.0
62-63	35.332856175347864	38.0	37.0	38.0	29.0	38.0
64-65	34.965367565570745	38.0	36.5	38.0	27.0	38.0
66-67	32.89391700529492	37.5	33.0	38.0	14.5	38.0
68-69	32.79891638960719	37.0	32.5	38.0	15.5	38.0
70-71	32.15152074867627	37.0	30.0	38.0	11.0	38.0
72-73	31.081193818495258	36.5	26.0	38.0	11.0	38.0
74-75	33.41060214259328	37.0	33.0	38.0	18.0	38.0
76	34.11267085334318	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	35.0
14	82.0
15	166.0
16	240.0
17	325.0
18	357.0
19	384.0
20	331.0
21	319.0
22	229.0
23	232.0
24	229.0
25	277.0
26	334.0
27	385.0
28	521.0
29	665.0
30	863.0
31	1272.0
32	1903.0
33	2895.0
34	4264.0
35	6094.0
36	6862.0
37	3215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.00541805196404	20.991872922053933	11.959734022903582	31.04297500307844
2	19.83438000246275	17.134589336288634	38.60977712104421	24.421253540204408
3	20.28075360177318	18.356729466814432	33.03472478758774	28.327792143824652
4	14.511759635512869	17.46090382957764	33.02856791035587	34.99876862455363
5	15.315232114271641	30.667405491934492	36.98743997044699	17.02992242334688
6	29.522226326807044	31.594015515330625	16.41423470016008	22.469523457702252
7	27.80137914049994	26.308336411771947	21.545991872922055	24.344292574806058
8	23.611624184213767	31.926486885851496	17.913434306119935	26.5484546238148
9	23.346878463243442	15.019702007141976	19.76357591429627	41.86984361531831
10-11	25.648011328654107	23.277613594384928	25.57566802117966	25.498707055781306
12-13	25.040019702007143	19.85900751139022	27.545868735377415	27.55510405122522
14-15	22.82046545991873	22.371013421992366	23.03595616303411	31.772564955054794
16-17	24.2211550301687	23.97026228297008	25.994335672946683	25.814247013914542
18-19	23.18526043590691	23.28223125230883	26.546915404506834	26.98559290727743
20-21	23.965644625046174	23.27607437507696	26.782415958625784	25.97586504125108
22-23	24.14265484546238	22.278660263514347	27.431966506587855	26.146718384435413
24-25	23.3114764191602	24.616734392316218	24.258096293559905	27.813692894963676
26-27	22.252493535278905	24.008742765669254	27.44735869966753	26.29140499938431
28-29	23.49156507819234	24.88609777121044	25.520256126092843	26.10208102450437
30-31	22.97900504863933	24.915342938061816	25.297069326437633	26.808582686861225
32-33	23.299162664696464	24.62904814677995	25.344785124984607	26.727004063538974
34-35	23.434613963797563	24.667528629479126	25.49101095924147	26.406846447481836
36-37	23.62547715798547	22.815847801994828	28.484792513237284	25.073882526782416
38-39	22.972848171407463	22.20016007880803	31.460103435537494	23.366888314247014
40-41	23.576222140130525	23.017485531338505	24.642901120551656	28.76339120797931
42-43	22.544945203792636	23.894840536879695	24.81529368304396	28.74492057628371
44-45	22.504925501785493	24.02413495874892	26.2267577884497	27.244181751015883
46-47	23.340721586011576	23.09136805812092	27.550486393301316	26.017423962566188
48-49	23.31301563846817	23.268378278537124	27.558182489841155	25.860423593153552
50-51	23.44077084102943	21.79842383942864	30.593522965152076	24.167282354389855
52-53	22.324836842753356	24.131880310306613	26.73316094077084	26.810121906169194
54-55	22.62960226573082	23.52850634158355	27.435044945203792	26.406846447481836
56-57	22.603435537495383	23.913311168575298	26.617719492673313	26.865533801256003
58-59	21.60140376800887	24.738332717645612	26.48842507080409	27.171838443541436
60-61	22.355621228912696	23.739379386775028	26.474572097032382	27.43042728727989
62-63	21.33204038911464	24.453577145671716	26.2406107622214	27.973771702992245
64-65	22.066247999014898	24.8322250954316	25.7788449698313	27.322681935722205
66-67	21.062677010220415	23.59315355251816	23.974879940893977	31.369289496367443
68-69	20.781059987993164	23.942859781721904	24.70637131905854	30.56970891122639
70-71	21.221216598941016	23.839428641792882	23.797869720477774	31.141485038788325
72-73	21.284324590567664	22.837396872306368	22.634219923654722	33.24405861347125
74-75	22.351003570988794	24.76296022657308	24.52130279522226	28.36473340721586
76	23.152936830439604	23.85482083487255	25.107745351557693	27.88449698313016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	0.5
16	3.0
17	5.0
18	5.5
19	5.5
20	8.5
21	12.0
22	12.5
23	16.5
24	39.5
25	59.0
26	60.5
27	94.5
28	138.0
29	149.0
30	185.0
31	278.0
32	354.5
33	374.0
34	473.5
35	662.0
36	866.0
37	981.0
38	1027.5
39	1204.0
40	1434.5
41	1550.0
42	1565.0
43	1680.5
44	1820.5
45	1853.0
46	1861.0
47	1796.0
48	1663.0
49	1516.5
50	1438.0
51	1355.0
52	1169.0
53	1006.5
54	947.0
55	864.0
56	724.5
57	604.5
58	541.0
59	532.0
60	514.5
61	513.5
62	521.0
63	514.0
64	533.5
65	580.0
66	606.5
67	613.0
68	614.0
69	619.5
70	576.0
71	528.0
72	506.5
73	463.0
74	387.5
75	334.0
76	300.5
77	238.0
78	177.0
79	145.0
80	121.5
81	81.5
82	53.5
83	42.0
84	33.0
85	19.0
86	10.5
87	7.0
88	5.5
89	4.0
90	2.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.007696096539834996
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	32484.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.36793498337644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.89117581965917	95.31461642654845
2	1.6820006955641973	3.2754586873537743
3	0.3319738214929337	0.9697081640192096
4	0.05690979797021721	0.2216475803472479
5	0.025293243542318756	0.12313754463735993
6	0.006323310885579689	0.03694126339120798
7	0.0031616554427898445	0.021549070311537986
8	0.0	0.0
9	0.0	0.0
>10	0.0031616554427898445	0.03694126339120798
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0030784386159339984	0.0
20	0.0	0.0	0.0	0.0030784386159339984	0.0
21	0.0	0.0	0.0	0.006156877231867997	0.0
22	0.0	0.0	0.0	0.030784386159339983	0.0
23	0.0	0.0	0.0	0.030784386159339983	0.0
24	0.0	0.0	0.0	0.04309814062307597	0.0
25	0.0	0.0	0.0	0.04309814062307597	0.0
26	0.0	0.0	0.0	0.04309814062307597	0.0
27	0.0	0.0	0.0	0.049255017854943975	0.0
28	0.0	0.0	0.0	0.058490333702745964	0.0
29	0.0	0.0	0.0	0.06464721093461397	0.0
30	0.0	0.0	0.0	0.06464721093461397	0.0
31	0.0	0.0	0.0	0.06464721093461397	0.0
32	0.0	0.0	0.0	0.06772564955054797	0.0
33	0.0	0.0	0.0	0.07388252678241596	0.0
34	0.0	0.0	0.0	0.09543159709395395	0.0
35	0.0	0.0	0.0	0.11698066740549193	0.0
36	0.0	0.0	0.0	0.1416081763329639	0.0
37	0.0	0.0	0.0	0.23088289619504987	0.0
38	0.0	0.0	0.0	0.30168698436153185	0.0
39	0.0	0.0	0.0	0.42174609038295774	0.0
40	0.0	0.0	0.0	0.6156877231867997	0.0
41	0.0	0.0	0.0	0.8619628124615195	0.0
42	0.0	0.0	0.0	1.1236300948159095	0.0
43	0.0	0.0	0.0	1.3729836227065633	0.0
44	0.0	0.0	0.0	1.5977096416697452	0.0
45	0.0	0.0	0.0	1.807043467553257	0.0
46	0.0	0.0	0.0	2.034847925132373	0.0
47	0.0	0.0	0.0	2.3396133481098387	0.0
48	0.0	0.0	0.0	2.5612609284570866	0.0
49	0.0	0.0	0.0	2.81061445634774	0.0
50	0.0	0.0	0.0	3.136928949636744	0.0
51	0.0	0.0	0.0	3.484792513237286	0.0
52	0.0	0.0	0.0	3.844969831301564	0.0
53	0.0	0.0	0.0	4.476049747568034	0.0
54	0.0	0.0	0.0	4.9501292944218696	0.0
55	0.0	0.0	0.0	5.282600664942741	0.0
56	0.0	0.0	0.0	5.584287649304273	0.0
57	0.0	0.0	0.0	5.781307720724048	0.0
58	0.0	0.0	0.0	6.009112178303164	0.0
59	0.0	0.0	0.0	6.286171653737225	0.0
60	0.0	0.0	0.0	6.486270163772934	0.0
61	0.0	0.0	0.0	6.7079177441201825	0.0
62	0.0	0.0	0.0	6.895702499692156	0.0
63	0.0	0.0	0.0	7.0988794483438	0.0
64	0.0	0.0	0.0	7.34515453761852	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCA	15	0.002205419	70.0	56
CGGCATT	15	0.002205419	70.0	65
ATTACGG	15	0.002205419	70.0	28
TACCTGA	15	0.002205419	70.0	58
TAGATCT	15	0.002205419	70.0	39
CATCGGC	15	0.002205419	70.0	62
ACCCCAA	15	0.002205419	70.0	40
GTCGCCG	20	0.006891618	52.499996	51
TTATGAT	20	0.006891618	52.499996	17
GGTCCTT	20	0.006891618	52.499996	42
TACGGAT	20	0.006891618	52.499996	30
AACATCG	20	0.006891618	52.499996	60
TATTACG	20	0.006891618	52.499996	27
CTTCTAG	20	0.006891618	52.499996	1
GGCATTA	20	0.006891618	52.499996	66
ATCGGCA	20	0.006891618	52.499996	63
GCTCGGA	20	0.006891618	52.499996	11
AAAATCC	20	0.006891618	52.499996	8
CTTTGAC	30	5.794888E-4	46.666664	9
CCCCTCT	50	1.6742019E-4	35.000004	7
>>END_MODULE