FastQCFastQC Report
Wed 25 May 2016
SRR1779693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32484
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT6031.8562984854082012No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2660.8188646718384436TruSeq Adapter, Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1210.3724910725280138No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1000.3078438615933998No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG960.2955301071296638No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC910.2801379140499939No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG370.11390222878955793No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT350.10774535155768994No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCCC150.00220541970.044
TCTACCC150.00220541970.040
AGGCATG150.00220541970.024
TGCGCCG200.00689161852.49999646
TCTTTAT200.00689161852.4999961
CTTTATA200.00689161852.4999962
CGTCAGA200.00689161852.4999962
AGCAGCG200.00689161852.4999967
CTGATTT350.001237825140.068
TCCTGGA400.002385070334.9999962
CGTATCT1800.033.05555334
GTATCTC1800.033.05555335
ACGTATC1800.033.05555333
CACGTAT1800.033.05555332
ATCTCGT2050.032.43902637
TCTCGTA2050.032.43902638
CGTCTTC2300.031.9565248
TATCTCG1900.031.31578836
AGCCCAT450.004247637531.1111097
CCGTCTT2400.030.62547