##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779693_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32484
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02678241595863	34.0	34.0	34.0	31.0	34.0
2	33.24298115995567	34.0	34.0	34.0	32.0	34.0
3	33.358884373845584	34.0	34.0	34.0	33.0	34.0
4	33.41192587119813	34.0	34.0	34.0	34.0	34.0
5	33.333702745967244	34.0	34.0	34.0	33.0	34.0
6	36.53786479497599	38.0	37.0	38.0	34.0	38.0
7	36.64921191971432	38.0	37.0	38.0	34.0	38.0
8	36.26062061322497	38.0	37.0	38.0	34.0	38.0
9	36.26723925624923	38.0	37.0	38.0	34.0	38.0
10-11	36.181212289126954	38.0	37.0	38.0	34.0	38.0
12-13	36.209672454131265	38.0	37.0	38.0	34.0	38.0
14-15	36.28424762960226	38.0	37.0	38.0	34.0	38.0
16-17	36.14714936584164	38.0	36.5	38.0	33.5	38.0
18-19	36.25078500184706	38.0	37.0	38.0	34.0	38.0
20-21	36.27752124122645	38.0	37.0	38.0	34.0	38.0
22-23	36.083302548947174	38.0	36.0	38.0	32.5	38.0
24-25	36.06512436892008	38.0	36.0	38.0	32.0	38.0
26-27	35.877555104051225	38.0	36.0	38.0	31.5	38.0
28-29	35.743304396010345	38.0	36.0	38.0	31.0	38.0
30-31	35.65410663711366	38.0	36.0	38.0	31.0	38.0
32-33	35.66685137298362	38.0	36.0	38.0	31.0	38.0
34-35	35.6650504863933	38.0	36.0	38.0	31.0	38.0
36-37	35.70656323112917	38.0	36.0	38.0	31.0	38.0
38-39	35.74131880310307	38.0	36.0	38.0	31.0	38.0
40-41	35.7820311537988	38.0	36.0	38.0	31.0	38.0
42-43	35.808828961950496	38.0	36.0	38.0	31.5	38.0
44-45	35.774658293313635	38.0	36.0	38.0	31.0	38.0
46-47	35.81258465706194	38.0	36.0	38.0	31.0	38.0
48-49	35.814077699790666	38.0	36.0	38.0	31.0	38.0
50-51	35.783108607314375	38.0	36.0	38.0	31.0	38.0
52-53	35.76343738455856	38.0	36.0	38.0	31.0	38.0
54-55	35.756618643024254	38.0	36.0	38.0	31.0	38.0
56-57	35.7357622214013	38.0	36.0	38.0	31.0	38.0
58-59	35.712520009851005	38.0	36.0	38.0	31.0	38.0
60-61	35.756695603989655	38.0	36.0	38.0	31.0	38.0
62-63	35.65305996798424	38.0	36.0	38.0	31.0	38.0
64-65	35.64345523950253	38.0	36.0	38.0	31.0	38.0
66-67	35.607145056027576	38.0	36.0	38.0	31.0	38.0
68-69	35.431735623691665	38.0	36.0	38.0	31.0	38.0
70-71	35.12744735869967	38.0	36.0	38.0	27.5	38.0
72-73	34.90813939170053	38.0	36.0	38.0	26.0	38.0
74-75	34.909801748553136	38.0	36.0	38.0	26.5	38.0
76	33.855005541189506	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	2.0
22	2.0
23	8.0
24	23.0
25	40.0
26	70.0
27	111.0
28	216.0
29	373.0
30	575.0
31	1023.0
32	1466.0
33	2223.0
34	3310.0
35	4906.0
36	7280.0
37	10855.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.908878216968354	21.28124615195173	12.209087550794237	26.60078808028568
2	21.38899150350942	19.806674054919345	35.37741657431351	23.42691786725773
3	20.82871567540943	23.670114517916513	28.438615933998275	27.062553872675778
4	15.832409801748554	18.135081886467184	34.786356360054185	31.246151951730084
5	16.164881172269425	36.119320280753605	33.047038542051475	14.668760004925502
6	33.45031400073882	34.78327792143825	16.21413619012437	15.55227188769856
7	30.824405861347127	27.792143824652136	20.945696342814923	20.437753971185817
8	25.646472109346142	35.66063292697944	18.202807536017733	20.490087427656693
9	24.732175840413742	17.220785617534787	19.979066617411647	38.06797192463982
10-11	27.608976727004062	24.52745967245413	25.056951114394778	22.806612486147028
12-13	26.880925994335676	20.694803595616303	25.31861839674917	27.105652013298855
14-15	24.684460041866764	22.197081640192096	21.57985469769733	31.538603620243812
16-17	26.473032877724417	26.35451299101096	22.52185691417313	24.65059721709149
18-19	26.508434921807662	24.402782908508804	23.560829947050856	25.52795222263268
20-21	25.073882526782416	24.296576776259084	23.18833887452284	27.441201822435662
22-23	25.620305381110704	25.735746829208228	23.600849649058	25.043098140623076
24-25	26.777798300701882	25.6156877231868	23.396133481098385	24.210380495012927
26-27	26.539219307967	26.7116118704593	23.542359315355252	23.206809506218445
28-29	27.238024873784017	24.35506710996183	24.110331239995077	24.296576776259084
30-31	27.04100480236424	25.39557936214752	23.57930057874646	23.98411525674178
32-33	25.381726388375814	25.900443295160695	23.751693141238764	24.966137175224727
34-35	26.731621721462872	24.638283462627754	24.58902844477281	24.04106637113656
36-37	25.89890407585273	25.175471001108242	25.443295160694497	23.482329762344538
38-39	24.66137175224726	24.468969338751386	26.125169314123873	24.74448959487748
40-41	24.592106883388745	23.226819357222016	27.182612978697207	24.998460780692035
42-43	26.16364979682305	23.21912326068218	26.526905553503262	24.090321388991505
44-45	24.262713951483807	24.842999630587368	26.71315108976727	24.181135328161556
46-47	26.045129910109594	23.280692033000864	25.607991626646964	25.06618643024258
48-49	25.855805935229654	22.71118088905307	25.72651151336042	25.70650166235685
50-51	23.98411525674178	23.208348725526413	27.26727004063539	25.540265977096414
52-53	23.928703361654968	22.77890653860362	28.88498953330871	24.407400566432706
54-55	23.82095801009728	22.96976973279153	27.798300701884003	25.410971555227192
56-57	24.983068587612365	22.509543159709395	26.77471986208595	25.732668390592288
58-59	23.602388868365964	24.28272380248738	28.075360177318064	24.039527151828594
60-61	24.54900874276567	25.777305750523333	25.709580100972783	23.96410540573821
62-63	22.769671222755818	28.889607191232606	24.61057751508435	23.730144070927224
64-65	22.78814185445142	29.39754956286172	23.63471247383327	24.17959610885359
66-67	23.288388129540696	28.59561630341091	23.400751139022287	24.715244428026107
68-69	22.23556212289127	29.537618519886717	23.639330131757173	24.587489225464846
70-71	22.720416204900875	28.41552764437877	24.06107622214013	24.802979928580225
72-73	23.252986085457454	26.645425440216723	23.71321265853959	26.388375815786237
74-75	23.305319541928334	26.81627878340106	23.771702992242336	26.10669868242827
76	23.580839798054427	27.03176948651644	23.534663218815417	25.85272749661372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	4.0
19	10.0
20	18.5
21	23.0
22	29.5
23	42.0
24	65.0
25	82.0
26	101.5
27	128.0
28	159.0
29	183.0
30	215.5
31	296.0
32	409.5
33	475.0
34	543.5
35	678.5
36	816.5
37	888.0
38	990.5
39	1212.5
40	1490.0
41	1804.0
42	1960.0
43	2113.0
44	2195.5
45	2041.0
46	1957.0
47	1824.5
48	1580.5
49	1348.0
50	1227.0
51	1163.0
52	966.0
53	786.0
54	739.0
55	679.0
56	574.0
57	488.5
58	448.0
59	393.0
60	357.5
61	397.0
62	417.0
63	415.0
64	444.0
65	473.5
66	496.5
67	521.0
68	546.0
69	582.5
70	611.5
71	629.0
72	573.5
73	508.0
74	470.0
75	442.0
76	390.5
77	301.0
78	239.5
79	216.0
80	183.0
81	126.5
82	80.0
83	57.0
84	48.5
85	35.0
86	20.5
87	11.0
88	9.0
89	5.0
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	32484.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.41275704962443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24632263702121	47.08164019209457
2	14.874508471285013	18.86467183844354
3	5.388611097626098	10.251200591060213
4	2.3739016457109567	6.021425932766901
5	1.1602504975969707	3.678734146041128
6	0.6844992475362881	2.6043590690801626
7	0.40778678576629934	1.810121906169191
8	0.24758483421525318	1.2560029553010712
9	0.174765765328414	0.9974141115626155
>10	0.41264139035875524	3.50326314493289
>50	0.014563813777367833	0.8835118827730575
>100	0.009709209184911888	1.1913557443664573
>500	0.004854604592455944	1.8562984854082012
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	603	1.8562984854082012	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	266	0.8188646718384436	TruSeq Adapter, Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	121	0.3724910725280138	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	100	0.3078438615933998	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	96	0.2955301071296638	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	91	0.2801379140499939	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	37	0.11390222878955793	No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT	35	0.10774535155768994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0030784386159339984	0.0
20	0.0	0.0	0.0	0.006156877231867997	0.0
21	0.0	0.0	0.0	0.006156877231867997	0.0
22	0.0	0.0	0.0	0.006156877231867997	0.0
23	0.0	0.0	0.0	0.009235315847801994	0.0
24	0.0	0.0	0.0	0.009235315847801994	0.0
25	0.0	0.0	0.0	0.012313754463735994	0.0
26	0.0	0.0	0.0	0.012313754463735994	0.0
27	0.0	0.0	0.0	0.01847063169560399	0.0
28	0.0	0.0	0.0	0.027705947543405985	0.0
29	0.0	0.0	0.0	0.030784386159339983	0.0
30	0.0	0.0	0.0	0.03694126339120798	0.0
31	0.0	0.0	0.0	0.04001970200714198	0.0
32	0.0	0.0	0.0	0.04309814062307597	0.0
33	0.0	0.0	0.0	0.049255017854943975	0.0
34	0.0	0.0	0.0	0.08927471986208595	0.0
35	0.0	0.0	0.0	0.12929442186922793	0.0
36	0.0	0.0	0.0	0.19394163280384188	0.0
37	0.0	0.0	0.0	0.3663341952961458	0.0
38	0.0	0.0	0.0	0.47100110823790176	0.0
39	0.0	0.0	0.0	0.6280014776505356	0.0
40	0.0	0.0	0.0	0.8588843738455855	0.0
41	0.0	0.0	0.0	1.1482576037433814	0.0
42	0.0	0.0	0.0	1.4838074128801872	0.0
43	0.0	0.0	0.0	1.816278783401059	0.0
44	0.0	0.0	0.0	2.114887329146657	0.0
45	0.0	0.0	0.0	2.4904568402906047	0.0
46	0.0	0.0	0.0	2.8691047900504865	0.0
47	0.0	0.0	0.0	3.281615564585642	0.0
48	0.0	0.0	0.0	3.654106637113656	0.0
49	0.0	0.0	0.0	4.054303657185076	0.0
50	0.0	0.0	0.0	4.436030045560892	0.0
51	0.0	0.0	0.0	4.814677995320773	0.0
52	0.0	0.0	0.0	5.273365349094939	0.0
53	0.0	0.0	0.0	5.9075237039773425	0.0
54	0.0	0.0	0.0	6.400073882526782	0.0
55	0.0	0.0	0.0	6.846447481837212	0.0
56	0.0	0.0	0.0	7.262036694988302	0.0
57	0.0	0.0	0.0	7.579115872429504	0.0
58	0.0	0.0	0.0	7.930057874645979	0.0
59	0.0	0.0	0.0	8.314862701637729	0.0
60	0.0	0.0	0.0	8.721216598941018	0.0
61	0.0	0.0	0.0	9.084472355621228	0.0
62	0.0	0.0	0.0	9.552395025243197	0.0
63	0.0	0.0	0.0	10.060337396872306	0.0
64	0.0	0.0	0.0	10.58675040019702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCCCC	15	0.002205419	70.0	44
TCTACCC	15	0.002205419	70.0	40
AGGCATG	15	0.002205419	70.0	24
TGCGCCG	20	0.006891618	52.499996	46
TCTTTAT	20	0.006891618	52.499996	1
CTTTATA	20	0.006891618	52.499996	2
CGTCAGA	20	0.006891618	52.499996	2
AGCAGCG	20	0.006891618	52.499996	7
CTGATTT	35	0.0012378251	40.0	68
TCCTGGA	40	0.0023850703	34.999996	2
CGTATCT	180	0.0	33.055553	34
GTATCTC	180	0.0	33.055553	35
ACGTATC	180	0.0	33.055553	33
CACGTAT	180	0.0	33.055553	32
ATCTCGT	205	0.0	32.439026	37
TCTCGTA	205	0.0	32.439026	38
CGTCTTC	230	0.0	31.95652	48
TATCTCG	190	0.0	31.315788	36
AGCCCAT	45	0.0042476375	31.111109	7
CCGTCTT	240	0.0	30.625	47
>>END_MODULE