##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779692_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43887
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62640417435687	34.0	32.0	34.0	21.0	34.0
2	28.47902567958621	34.0	23.0	34.0	12.0	34.0
3	27.645680953357488	33.0	23.0	34.0	11.0	34.0
4	30.718868913345638	34.0	32.0	34.0	23.0	34.0
5	29.803062410280948	34.0	31.0	34.0	11.0	34.0
6	32.58892154852234	37.0	31.0	38.0	12.0	38.0
7	33.621322943012736	37.0	34.0	38.0	22.0	38.0
8	32.88433932599631	37.0	33.0	38.0	12.0	38.0
9	34.5350103675348	38.0	34.0	38.0	26.0	38.0
10-11	34.27713218037232	38.0	34.5	38.0	24.0	38.0
12-13	32.999783534987586	37.5	31.0	38.0	19.0	38.0
14-15	33.332923189099276	37.0	33.0	38.0	21.5	38.0
16-17	32.92415749538588	37.5	31.0	38.0	18.5	38.0
18-19	33.31341855219085	37.0	33.0	38.0	22.0	38.0
20-21	32.3267026682161	36.5	30.0	38.0	17.0	38.0
22-23	32.32125686421948	36.5	29.0	38.0	18.0	38.0
24-25	33.97542552464283	37.0	34.0	38.0	24.5	38.0
26-27	32.077995761842914	37.0	29.5	38.0	16.0	38.0
28-29	34.737826691275316	37.5	35.0	38.0	26.5	38.0
30-31	35.220087953152415	38.0	36.0	38.0	27.0	38.0
32-33	35.337879098594115	38.0	37.0	38.0	29.0	38.0
34-35	35.41273042130927	38.0	37.0	38.0	31.0	38.0
36-37	32.651160936040284	37.0	30.5	38.0	17.0	38.0
38-39	30.342869186775125	35.0	25.0	38.0	11.0	38.0
40-41	32.17728484517055	36.5	29.5	38.0	16.0	38.0
42-43	33.5178640599722	37.0	32.5	38.0	19.0	38.0
44-45	35.389261056804976	38.0	36.5	38.0	31.0	38.0
46-47	33.539111809875365	37.5	32.0	38.0	21.0	38.0
48-49	32.76200241529382	37.0	30.5	38.0	18.0	38.0
50-51	30.2265363319434	35.0	24.5	38.0	11.0	38.0
52-53	34.431540091598876	37.0	35.0	38.0	25.5	38.0
54-55	33.290234010071316	37.0	31.5	38.0	19.0	38.0
56-57	33.78416159682821	37.5	33.5	38.0	20.0	38.0
58-59	35.199330097751044	38.0	36.0	38.0	29.0	38.0
60-61	35.68200150386219	38.0	37.0	38.0	31.0	38.0
62-63	35.51388793948094	38.0	37.0	38.0	29.5	38.0
64-65	35.14712785107207	38.0	36.5	38.0	27.5	38.0
66-67	32.75656800419259	37.0	32.5	38.0	15.0	38.0
68-69	32.72732244172534	37.0	32.5	38.0	16.0	38.0
70-71	32.1249344908515	37.0	29.5	38.0	15.0	38.0
72-73	30.80757399685556	35.5	26.0	38.0	11.0	38.0
74-75	33.40630482830906	37.0	32.5	38.0	22.5	38.0
76	34.13915282429877	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	18.0
14	49.0
15	95.0
16	177.0
17	241.0
18	313.0
19	333.0
20	337.0
21	324.0
22	334.0
23	396.0
24	407.0
25	485.0
26	552.0
27	783.0
28	884.0
29	1245.0
30	1649.0
31	2260.0
32	3029.0
33	4447.0
34	6039.0
35	8014.0
36	8004.0
37	3472.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41515710802743	19.727937658076424	11.607081823774694	28.24982341012145
2	21.75587303757377	16.531091211520497	38.37127167498348	23.341764075922256
3	21.605486818420033	18.527126483924626	33.028003736869685	26.839382960785652
4	15.389523093398957	16.80224212181284	32.66343108437578	35.144803700412425
5	15.45332330758539	30.20028710096384	36.919816802242124	17.42657278920865
6	30.535238225442612	31.998085993574403	15.890810490578074	21.575865290404902
7	28.71921070020735	25.766172215006723	20.64620502654545	24.86841205824048
8	24.84562626745961	31.061589992480688	18.16255383143072	25.930229908628977
9	24.344338870280495	14.530498780960194	19.8190808211999	41.306081527559414
10-11	28.23843051473101	22.511222001959577	24.887779980404222	24.36256750290519
12-13	26.242694222596924	19.302063276861904	26.786141524158907	27.66910097638226
14-15	25.097409255588214	21.01077767903935	22.731104882994966	31.160708182377466
16-17	25.98605509604211	22.603504454622097	25.55540365028368	25.85503679905211
18-19	25.050698384487436	22.408685943445665	25.526921411807596	27.013694260259303
20-21	25.019937566933258	22.53058992412332	26.005423018205846	26.444049490737576
22-23	25.75477931961629	21.723972930480553	26.50784970492401	26.01339804497915
24-25	25.409574589286123	23.401007131952515	22.96124136988174	28.22817690887962
26-27	24.30674231549206	22.180828035636978	26.83482580262948	26.67760384624148
28-29	25.98719438558115	23.559368377879554	24.30674231549206	26.146694921047235
30-31	25.630596759860552	23.385057078405904	23.70633672841616	27.278009433317386
32-33	25.254631211976214	23.691525964408594	24.03673069473876	27.01711212887643
34-35	25.54515004443229	23.684690227174336	24.24066352222754	26.529496206165838
36-37	25.419828195137512	21.43915054571969	27.61637842641329	25.524642832729512
38-39	24.770433157882742	21.30471438011256	30.364344794586096	23.560507667418598
40-41	25.533757149041858	22.234374643972018	23.402146421491558	28.829721785494566
42-43	24.576753936245357	22.892883997539133	23.553671930184336	28.97669013603117
44-45	24.764736710187528	22.988584318818784	24.642832729509877	27.603846241483808
46-47	25.02677330416752	21.869801991478113	26.899765306354954	26.203659397999406
48-49	25.31273497846743	22.203613826417843	26.227584478319322	26.256066716795406
50-51	24.190534782509626	20.972041834711874	29.991797115318885	24.84562626745961
52-53	24.761318841570397	23.05352382254426	24.925376535192655	27.25978080069269
54-55	24.65992207259553	22.587554401075487	25.775286531319068	26.97723699500991
56-57	24.256613575774146	22.3938751794381	25.93478706678515	27.414724178002597
58-59	24.514093011597968	22.922505525554264	25.137284389454734	27.42611707339303
60-61	24.724861576321004	22.68667259097227	24.68612573199353	27.902340100713197
62-63	24.011666324879805	23.22099938478365	25.132727231298563	27.634607059037986
64-65	24.539157381456924	23.9774876387085	24.302185157335884	27.181169822498692
66-67	23.160617039214344	22.933898420944697	22.816551598423224	31.08893294141773
68-69	22.91915911809947	23.44556486070757	23.31681194097875	30.31846408021421
70-71	23.087702508715566	22.839337389204093	23.274545993118693	30.79841410896165
72-73	22.839337389204093	22.012213183858545	21.801444619135506	33.34700480780185
74-75	24.154077517260237	23.63000432930025	23.881787317428852	28.33413083601066
76	24.91170506072413	22.78579078086905	24.084580855378587	28.21792330302823
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.5
17	3.0
18	5.0
19	5.0
20	8.5
21	14.0
22	20.0
23	27.0
24	44.0
25	60.0
26	73.5
27	100.5
28	147.0
29	180.0
30	217.5
31	311.5
32	424.0
33	480.0
34	534.5
35	668.5
36	897.5
37	1047.0
38	1139.5
39	1393.0
40	1619.5
41	1764.0
42	1843.0
43	1917.0
44	2034.0
45	2049.5
46	2022.0
47	2039.5
48	2010.0
49	1835.0
50	1707.0
51	1605.5
52	1447.5
53	1318.5
54	1246.0
55	1205.0
56	1160.5
57	1139.5
58	1122.0
59	1150.0
60	1177.0
61	1220.5
62	1265.0
63	1262.0
64	1282.0
65	1275.5
66	1232.0
67	1218.0
68	1185.0
69	1067.0
70	886.5
71	791.0
72	737.5
73	611.0
74	484.0
75	430.0
76	375.5
77	278.0
78	189.0
79	143.0
80	118.0
81	84.0
82	52.0
83	29.0
84	23.5
85	16.5
86	11.0
87	7.0
88	3.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0011392895390434524
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.010253605851391073
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	43887.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.74420671269397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.13739888570296	95.92362202930252
2	1.52691330396065	2.9849385922938456
3	0.25642818845140686	0.7519310957686787
4	0.05594796838939786	0.2187435914963429
5	0.01864932279646595	0.0911431631234762
6	0.002331165349558244	0.013671474468521431
7	0.002331165349558244	0.015950053546608334
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.002278579078086905	0.0	0.0	0.0
17	0.0	0.002278579078086905	0.0	0.0	0.0
18	0.0	0.002278579078086905	0.0	0.0	0.0
19	0.0	0.002278579078086905	0.0	0.006835737234260715	0.0
20	0.0	0.002278579078086905	0.0	0.00911431631234762	0.0
21	0.0	0.002278579078086905	0.0	0.011392895390434525	0.0
22	0.0	0.002278579078086905	0.0	0.01822863262469524	0.0
23	0.0	0.002278579078086905	0.0	0.01822863262469524	0.0
24	0.0	0.002278579078086905	0.0	0.025064369858955955	0.0
25	0.0	0.002278579078086905	0.0	0.029621528015129767	0.0
26	0.0	0.002278579078086905	0.0	0.029621528015129767	0.0
27	0.0	0.002278579078086905	0.0	0.04329300248365119	0.0
28	0.0	0.002278579078086905	0.0	0.04785016063982501	0.0
29	0.0	0.002278579078086905	0.0	0.05012873971791191	0.0
30	0.0	0.002278579078086905	0.0	0.05012873971791191	0.0
31	0.0	0.002278579078086905	0.0	0.05012873971791191	0.0
32	0.0	0.006835737234260715	0.0	0.05468589787408572	0.0
33	0.0	0.006835737234260715	0.0	0.05924305603025953	0.0
34	0.0	0.006835737234260715	0.0	0.06607879326452025	0.0
35	0.0	0.006835737234260715	0.0	0.08658600496730239	0.0
36	0.0	0.006835737234260715	0.0	0.12532184929477977	0.0
37	0.0	0.006835737234260715	0.0	0.19823637979356074	0.0
38	0.0	0.006835737234260715	0.0	0.2711509102923417	0.0
39	0.0	0.006835737234260715	0.0	0.38052270604051314	0.0
40	0.0	0.006835737234260715	0.0	0.5217946088819012	0.0
41	0.0	0.006835737234260715	0.0	0.6767379861918108	0.0
42	0.0	0.006835737234260715	0.0	0.8567457333606763	0.0
43	0.0	0.006835737234260715	0.0	1.0276391642171943	0.0
44	0.0	0.006835737234260715	0.0	1.2304327021669288	0.0
45	0.0	0.006835737234260715	0.0	1.389933237633012	0.0
46	0.0	0.006835737234260715	0.0	1.5790553011142252	0.0
47	0.0	0.006835737234260715	0.0	1.772734522751612	0.0
48	0.0	0.006835737234260715	0.0	1.94362795360813	0.0
49	0.0	0.006835737234260715	0.0	2.1031284890742135	0.0
50	0.0	0.006835737234260715	0.0	2.333264975960991	0.0
51	0.0	0.006835737234260715	0.0	2.654544625971244	0.0
52	0.0	0.006835737234260715	0.0	2.902909745482717	0.0
53	0.0	0.006835737234260715	0.0	3.190010709321667	0.0
54	0.0	0.006835737234260715	0.0	3.506733201175747	0.0
55	0.0	0.006835737234260715	0.0	3.723198213594003	0.0
56	0.0	0.006835737234260715	0.0	3.989791965730171	0.0
57	0.0	0.006835737234260715	0.0	4.251828559710165	0.0
58	0.0	0.006835737234260715	0.0	4.504750837377811	0.0
59	0.0	0.006835737234260715	0.0	4.796408959372935	0.0
60	0.0	0.006835737234260715	0.0	4.987809601932235	0.0
61	0.0	0.006835737234260715	0.0	5.174653086335361	0.0
62	0.0	0.006835737234260715	0.0	5.29313919839588	0.0
63	0.0	0.006835737234260715	0.0	5.4959327363456145	0.0
64	0.0	0.006835737234260715	0.0	5.657711850889785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTC	15	0.0022106215	70.00001	39
CTGCGCT	15	0.0022106215	70.00001	4
CAGATCT	15	0.0022106215	70.00001	7
TGTAGAT	25	2.7956557E-6	70.00001	37
ATGGCTG	20	0.00690775	52.500004	32
GATCCGG	20	0.00690775	52.500004	6
ACCCTGC	20	0.00690775	52.500004	8
CCGCTCC	20	0.00690775	52.500004	9
CGTGTAT	20	0.00690775	52.500004	35
TGGGTTG	35	0.0012426084	40.000004	57
CGACGAA	45	0.0042638527	31.111113	22
GTGTAGA	50	0.0071361666	28.000002	36
CCGACGA	100	1.3593051E-5	24.500002	21
TGCCGAC	90	0.0051979367	19.444445	19
CTGCCGA	95	0.007103297	18.421053	18
>>END_MODULE