FastQCFastQC Report
Wed 25 May 2016
SRR1779692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43887
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT4991.1370109599653657No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT1970.4488800783831203RNA PCR Primer, Index 47 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG910.20735069610590837No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC870.19823637979356074No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC670.15266479823182263No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC580.13215758652904047No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCT200.0069077552.50000450
TGGACGC200.0069077552.5000045
TCCTGAG200.0069077552.50000445
CTGAGCG200.0069077552.50000447
TAGTTCT200.0069077552.50000445
CTAACTA200.0069077552.50000423
CAGGATA200.0069077552.50000420
GACGGTT200.0069077552.50000432
CACACCA200.0069077552.50000435
TTCGCCG200.0069077552.50000449
TCAAGGG200.0069077552.5000045
CCTTGGC200.0069077552.50000438
TAACTAC200.0069077552.50000424
ACGGTTT200.0069077552.50000433
CAAGACC400.002394230635.0000044
ATTTCCT400.002394230635.00000442
CGGGCAG450.004263852731.11111316
GTATTAG500.007136166628.0000021
TAGGATG500.007136166628.0000025
TAGATCT1508.240022E-1025.66666834
Produced by FastQC (version 0.11.5)