##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779692_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43887
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.15011278966436	34.0	34.0	34.0	32.0	34.0
2	33.33896142365621	34.0	34.0	34.0	33.0	34.0
3	33.39544739900198	34.0	34.0	34.0	34.0	34.0
4	33.45428031079819	34.0	34.0	34.0	34.0	34.0
5	33.399024768154575	34.0	34.0	34.0	34.0	34.0
6	36.64114658099209	38.0	37.0	38.0	34.0	38.0
7	36.72718572698066	38.0	37.0	38.0	34.0	38.0
8	36.3828012851186	38.0	37.0	38.0	34.0	38.0
9	36.45703739148267	38.0	37.0	38.0	34.0	38.0
10-11	36.32102900631166	38.0	37.0	38.0	34.0	38.0
12-13	36.3663613370702	38.0	37.0	38.0	34.0	38.0
14-15	36.43662132294301	38.0	37.0	38.0	34.0	38.0
16-17	36.31101465126348	38.0	37.0	38.0	34.0	38.0
18-19	36.36769430583088	38.0	37.0	38.0	34.0	38.0
20-21	36.41173923941031	38.0	37.0	38.0	34.0	38.0
22-23	36.191389249663914	38.0	37.0	38.0	34.0	38.0
24-25	36.2052202246679	38.0	37.0	38.0	34.0	38.0
26-27	36.07405382003782	38.0	37.0	38.0	33.0	38.0
28-29	35.966208672271975	38.0	36.5	38.0	32.0	38.0
30-31	35.85584569462483	38.0	36.0	38.0	31.0	38.0
32-33	35.89220042381571	38.0	36.0	38.0	32.0	38.0
34-35	35.89033198897168	38.0	36.0	38.0	32.0	38.0
36-37	35.96242623100235	38.0	36.5	38.0	32.0	38.0
38-39	35.964602274021914	38.0	37.0	38.0	32.0	38.0
40-41	35.97802310479185	38.0	37.0	38.0	32.0	38.0
42-43	35.9592703989792	38.0	36.0	38.0	32.0	38.0
44-45	35.92762093558457	38.0	36.0	38.0	32.0	38.0
46-47	35.994201016246265	38.0	37.0	38.0	32.5	38.0
48-49	35.966823888623054	38.0	36.5	38.0	32.0	38.0
50-51	35.96328069815663	38.0	36.5	38.0	32.0	38.0
52-53	35.96393009319389	38.0	36.5	38.0	32.0	38.0
54-55	35.930753981816935	38.0	36.5	38.0	32.0	38.0
56-57	35.962585731537814	38.0	36.5	38.0	32.0	38.0
58-59	35.87797069747305	38.0	36.0	38.0	32.0	38.0
60-61	35.95085104928567	38.0	36.0	38.0	32.0	38.0
62-63	35.832547223551394	38.0	36.0	38.0	31.5	38.0
64-65	35.81885296329209	38.0	36.0	38.0	31.5	38.0
66-67	35.762811310866546	38.0	36.0	38.0	31.0	38.0
68-69	35.68099892906783	38.0	36.0	38.0	31.0	38.0
70-71	35.568437122610334	38.0	36.0	38.0	31.0	38.0
72-73	35.52552008567457	38.0	36.5	38.0	31.0	38.0
74-75	35.560131701870716	38.0	37.0	38.0	31.0	38.0
76	34.56021145213845	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	1.0
22	4.0
23	10.0
24	27.0
25	40.0
26	87.0
27	141.0
28	292.0
29	465.0
30	685.0
31	1144.0
32	1732.0
33	2663.0
34	3995.0
35	5891.0
36	9271.0
37	17435.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.29468863216898	20.616583498530318	11.771139517396952	26.317588351903755
2	22.10905279467724	20.122131838585457	32.55861644678378	25.21019891995352
3	21.657894137216033	22.273110488299498	27.878415020393284	28.19058035409119
4	16.18930434980746	17.576959008362387	33.33789049148951	32.895846150340645
5	16.948071182810402	34.09665732449245	32.3991159113177	16.556155581379453
6	33.467769498940456	33.713856039373844	16.893385284936315	15.92498917674938
7	30.218515733588536	27.766764645567026	20.308975322988584	21.70574429785586
8	26.00086586004967	34.258436439036615	18.224075466539066	21.516622234374644
9	25.137284389454734	15.369015881696175	19.87832387723016	39.61537585161893
10-11	28.755667965456745	23.22897441155695	24.13812746371363	23.877230159272678
12-13	27.510424499282248	19.621983730945384	24.38307471460797	28.4845170551644
14-15	25.589582336454985	21.246610613621346	21.253446350855608	31.910360699068065
16-17	27.325859593957208	24.55738601408162	21.834484015767767	26.282270376193406
18-19	26.96698338915852	23.65051154100303	22.867819627680177	26.51468544215827
20-21	25.50185704194864	23.845330052179463	22.487296921639665	28.165515984232233
22-23	26.614942921594093	24.31699592134345	22.611479481395403	26.456581675667053
24-25	27.324720304418165	24.53801809191788	22.265135461526192	25.872126142137763
26-27	26.327841957755144	24.991455328457175	23.03073803176339	25.64996468202429
28-29	25.890354774762457	24.5437145396131	23.855583658030852	25.710347027593595
30-31	26.30961332513045	23.792922733383463	24.236106364071368	25.661357577414723
32-33	27.2050949028186	23.776972679836852	23.740515414587463	25.27741700275708
34-35	26.753936245357394	24.01052703534076	24.51637159067605	24.71916512862579
36-37	27.145851846788343	24.07204867044911	23.11846332626974	25.663636156492807
38-39	25.622621733087247	23.40556429010869	24.509535853441793	26.46227812336227
40-41	25.64312894479003	22.995420056053046	24.763597420648484	26.597853578508442
42-43	26.69697176840522	23.025041584068177	24.486750062660924	25.79123658486568
44-45	25.531478569963774	23.715451044728507	24.85815845238909	25.894911932918628
46-47	26.585321393578965	22.68097614327705	24.18825620343154	26.545446259712442
48-49	26.270877480802973	22.52147560781097	24.12217741016702	27.085469501219038
50-51	25.620343154009163	22.457675393624534	25.021076856472302	26.900904595893998
52-53	24.841069109303437	22.711736960831225	26.1011233394855	26.34607059037984
54-55	25.00056964476952	22.901998313851482	25.394763825278556	26.702668216100438
56-57	25.690979105429857	22.823387335657483	24.91740150841935	26.56823205049331
58-59	24.761318841570397	23.362271287625035	25.841365324583588	26.035044546220977
60-61	25.71718276482785	24.09597375076902	24.216738441907626	25.9701050424955
62-63	23.975209059630416	25.75591860915533	23.822544261398594	26.44632806981566
64-65	24.268006471164583	26.43607446396427	22.90427689292957	26.391642171941577
66-67	24.764736710187528	25.522364253651425	23.011370109599653	26.701528926561398
68-69	24.130152436940325	26.382527855629228	23.117324036730693	26.36999567069975
70-71	23.909130266365892	26.071501811470366	23.03073803176339	26.988629890400347
72-73	24.282817235172146	24.792079659124568	23.15833846013626	27.766764645567026
74-75	24.004830587645547	24.85815845238909	23.7598833367512	27.377127623214164
76	24.37623897737371	24.731697313555266	23.428350080889558	27.46371362818147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	0.0
13	0.0
14	0.5
15	3.0
16	5.0
17	5.0
18	6.0
19	8.5
20	11.5
21	13.0
22	22.0
23	47.0
24	83.0
25	103.0
26	118.5
27	154.5
28	201.5
29	228.0
30	263.5
31	323.5
32	467.5
33	587.0
34	630.5
35	711.5
36	863.5
37	978.0
38	1121.5
39	1609.5
40	2047.5
41	2076.5
42	2012.0
43	2012.0
44	2054.0
45	2043.0
46	1990.0
47	1938.5
48	1765.5
49	1615.5
50	1587.0
51	1492.0
52	1252.5
53	1094.5
54	1081.0
55	1039.5
56	959.5
57	940.0
58	959.0
59	936.5
60	978.0
61	1053.5
62	1065.0
63	1117.5
64	1194.0
65	1225.5
66	1241.0
67	1249.0
68	1183.0
69	1102.5
70	993.5
71	899.0
72	865.5
73	754.5
74	596.0
75	515.0
76	465.0
77	384.5
78	288.5
79	223.0
80	195.0
81	133.0
82	84.0
83	69.0
84	53.0
85	29.0
86	16.5
87	12.0
88	7.5
89	2.5
90	1.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	43887.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.61109212295213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7304868001576	45.628546038690274
2	17.358598703299066	22.083988424818283
3	5.505605903213096	10.506528129058719
4	2.2889278933982875	5.82404812359013
5	1.1784933911236881	3.748262583452959
6	0.6770068417093527	2.58390867455055
7	0.44775584769137083	1.9937566933260418
8	0.2686535086148225	1.367147446852143
9	0.14686391804276963	0.840795679814068
>10	0.3761149120607515	3.1467177068380163
>50	0.014328187126123867	0.6904094606603322
>100	0.007164093563061934	1.5858910383484859
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	499	1.1370109599653657	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	197	0.4488800783831203	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	91	0.20735069610590837	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	87	0.19823637979356074	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	67	0.15266479823182263	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	58	0.13215758652904047	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.002278579078086905	0.0	0.0
8	0.0	0.0	0.002278579078086905	0.0	0.0
9	0.0	0.0	0.002278579078086905	0.0	0.0
10	0.0	0.0	0.002278579078086905	0.0	0.0
11	0.0	0.0	0.002278579078086905	0.0	0.0
12	0.0	0.0	0.002278579078086905	0.0	0.0
13	0.0	0.0	0.002278579078086905	0.0	0.0
14	0.0	0.0	0.002278579078086905	0.0	0.0
15	0.0	0.0	0.002278579078086905	0.0	0.0
16	0.0	0.0	0.002278579078086905	0.0	0.0
17	0.0	0.0	0.002278579078086905	0.0	0.0
18	0.0	0.0	0.002278579078086905	0.0	0.0
19	0.0	0.0	0.002278579078086905	0.00911431631234762	0.0
20	0.0	0.0	0.002278579078086905	0.011392895390434525	0.0
21	0.0	0.0	0.002278579078086905	0.015950053546608334	0.0
22	0.0	0.0	0.002278579078086905	0.015950053546608334	0.0
23	0.0	0.0	0.002278579078086905	0.015950053546608334	0.0
24	0.0	0.0	0.002278579078086905	0.015950053546608334	0.0
25	0.0	0.0	0.002278579078086905	0.015950053546608334	0.0
26	0.0	0.0	0.002278579078086905	0.01822863262469524	0.0
27	0.0	0.0	0.002278579078086905	0.025064369858955955	0.0
28	0.0	0.0	0.002278579078086905	0.03190010709321667	0.0
29	0.0	0.0	0.002278579078086905	0.03645726524939048	0.0
30	0.0	0.0	0.002278579078086905	0.04101442340556429	0.0
31	0.0	0.0	0.002278579078086905	0.0455715815617381	0.0
32	0.0	0.0	0.002278579078086905	0.06607879326452025	0.0
33	0.0	0.0	0.002278579078086905	0.07063595142069405	0.0
34	0.0	0.0	0.002278579078086905	0.08658600496730239	0.0
35	0.0	0.0	0.002278579078086905	0.1298790074509536	0.0
36	0.0	0.0	0.002278579078086905	0.26203659397999407	0.0
37	0.0	0.0	0.002278579078086905	0.38507986419668694	0.0
38	0.0	0.0	0.002278579078086905	0.5217946088819012	0.0
39	0.0	0.0	0.002278579078086905	0.6926880397384191	0.0
40	0.0	0.0	0.002278579078086905	0.9091530521566751	0.0
41	0.0	0.0	0.002278579078086905	1.1119465901064096	0.0
42	0.0	0.0	0.002278579078086905	1.3717046050083168	0.0
43	0.0	0.0	0.002278579078086905	1.6200697245197895	0.0
44	0.0	0.0	0.002278579078086905	1.9276779000615216	0.0
45	0.0	0.0	0.002278579078086905	2.2603504454622096	0.0
46	0.0	0.0	0.002278579078086905	2.645430309658897	0.0
47	0.0	0.0	0.002278579078086905	2.927974115341673	0.0
48	0.0	0.0	0.002278579078086905	3.208239341946362	0.0
49	0.0	0.0	0.002278579078086905	3.5636976781279195	0.0
50	0.0	0.0	0.002278579078086905	3.859912958279217	0.0
51	0.0	0.0	0.002278579078086905	4.251828559710165	0.0
52	0.0	0.0	0.002278579078086905	4.609565474969809	0.0
53	0.0	0.0	0.002278579078086905	4.942238020370497	0.0
54	0.0	0.0	0.002278579078086905	5.270353407615011	0.0
55	0.0	0.0	0.002278579078086905	5.596190215781439	0.0
56	0.0	0.0	0.002278579078086905	5.990384396290473	0.0
57	0.0	0.0	0.002278579078086905	6.3731856814090735	0.0
58	0.0	0.0	0.002278579078086905	6.767379861918108	0.0
59	0.0	0.0	0.002278579078086905	7.195752728598446	0.0
60	0.0	0.0	0.002278579078086905	7.603618383576002	0.0
61	0.0	0.0	0.002278579078086905	8.00236972224121	0.0
62	0.0	0.0	0.002278579078086905	8.330485109485725	0.0
63	0.0	0.0	0.002278579078086905	8.75430081800989	0.0
64	0.0	0.0	0.002278579078086905	9.132544944972315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCT	20	0.00690775	52.500004	50
TGGACGC	20	0.00690775	52.500004	5
TCCTGAG	20	0.00690775	52.500004	45
CTGAGCG	20	0.00690775	52.500004	47
TAGTTCT	20	0.00690775	52.500004	45
CTAACTA	20	0.00690775	52.500004	23
CAGGATA	20	0.00690775	52.500004	20
GACGGTT	20	0.00690775	52.500004	32
CACACCA	20	0.00690775	52.500004	35
TTCGCCG	20	0.00690775	52.500004	49
TCAAGGG	20	0.00690775	52.500004	5
CCTTGGC	20	0.00690775	52.500004	38
TAACTAC	20	0.00690775	52.500004	24
ACGGTTT	20	0.00690775	52.500004	33
CAAGACC	40	0.0023942306	35.000004	4
ATTTCCT	40	0.0023942306	35.000004	42
CGGGCAG	45	0.0042638527	31.111113	16
GTATTAG	50	0.0071361666	28.000002	1
TAGGATG	50	0.0071361666	28.000002	5
TAGATCT	150	8.240022E-10	25.666668	34
>>END_MODULE