##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779691_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11543 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.397903491293423 34.0 23.0 34.0 12.0 34.0 2 27.79788616477519 34.0 23.0 34.0 12.0 34.0 3 27.297842848479597 32.0 23.0 34.0 11.0 34.0 4 29.458806202893527 34.0 27.0 34.0 12.0 34.0 5 28.933379537381963 34.0 23.0 34.0 11.0 34.0 6 31.522914320367324 37.0 27.0 38.0 11.0 38.0 7 32.50524127176644 37.0 31.0 38.0 12.0 38.0 8 31.691674607987526 37.0 27.0 38.0 11.0 38.0 9 32.99688122671749 38.0 34.0 38.0 12.0 38.0 10-11 32.813523347483326 38.0 34.0 38.0 11.0 38.0 12-13 31.917482456900288 37.5 30.0 38.0 11.0 38.0 14-15 31.8271679805943 37.0 29.0 38.0 11.5 38.0 16-17 31.492939443818763 37.0 28.5 38.0 11.0 38.0 18-19 32.07970198388634 37.0 29.5 38.0 11.5 38.0 20-21 31.110110023390803 36.5 27.0 38.0 11.0 38.0 22-23 31.156761673741663 36.0 28.0 38.0 11.0 38.0 24-25 32.3546738282942 37.0 30.5 38.0 11.0 38.0 26-27 30.839946287793467 36.5 25.5 38.0 11.0 38.0 28-29 32.87975396344105 37.5 33.5 38.0 11.0 38.0 30-31 33.282422247249414 38.0 34.0 38.0 11.0 38.0 32-33 33.332149354587195 38.0 34.0 38.0 11.0 38.0 34-35 33.36160443558867 38.0 34.0 38.0 11.0 38.0 36-37 31.534003292038463 37.0 27.5 38.0 11.0 38.0 38-39 29.9733604782119 35.0 24.5 38.0 11.0 38.0 40-41 30.99484536082474 36.5 26.5 38.0 11.0 38.0 42-43 31.930520661872997 37.0 30.0 38.0 11.0 38.0 44-45 33.347180109157065 38.0 34.0 38.0 11.0 38.0 46-47 31.961318548037774 37.5 29.0 38.0 11.0 38.0 48-49 31.409858788876374 36.5 28.0 38.0 11.0 38.0 50-51 29.44802044529152 35.0 22.5 38.0 11.0 38.0 52-53 32.5677033700078 37.0 32.5 38.0 11.0 38.0 54-55 31.709607554361952 37.0 28.5 38.0 11.0 38.0 56-57 32.14775188425886 37.5 29.5 38.0 11.0 38.0 58-59 33.05899679459412 38.0 34.0 38.0 11.0 38.0 60-61 33.46183834358486 38.0 35.0 38.0 11.0 38.0 62-63 33.45586069479339 38.0 34.5 38.0 11.0 38.0 64-65 33.1092003811834 38.0 34.5 38.0 11.0 38.0 66-67 31.517976262670018 37.0 26.5 38.0 11.0 38.0 68-69 31.311747379364117 37.0 26.0 38.0 11.0 38.0 70-71 30.790175864160098 36.5 25.0 38.0 11.0 38.0 72-73 29.8402495018626 35.0 23.5 38.0 11.0 38.0 74-75 31.730659274018883 36.5 29.0 38.0 11.0 38.0 76 32.18695313176817 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 27.0 14 80.0 15 156.0 16 205.0 17 306.0 18 340.0 19 314.0 20 279.0 21 190.0 22 154.0 23 111.0 24 108.0 25 94.0 26 100.0 27 105.0 28 126.0 29 158.0 30 226.0 31 347.0 32 495.0 33 808.0 34 1254.0 35 1845.0 36 2370.0 37 1341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.49337260677467 19.743567530104826 12.024603655895348 32.738456207225155 2 19.067833318894568 17.057957203499956 40.75197089144936 23.12223858615611 3 20.913107511045656 16.633457506714024 35.7879234167894 26.665511565450924 4 14.398336654249327 18.842588581824483 32.56519102486355 34.193883739062635 5 14.017153253053799 27.895694360218315 40.032920384648705 18.054232002079182 6 26.44893008749892 33.24092523607381 17.005977648791475 23.304167027635796 7 25.0108290738976 26.752144156631726 20.479944555141643 27.75708221432903 8 21.424239799012387 32.09737503248722 17.880966819717578 28.59741834878281 9 21.93537208697912 15.975049813739927 19.89084293511219 42.19873516416876 10-11 23.338820064108116 24.075197089144936 27.055358225764532 25.53062462098241 12-13 22.957636662912588 19.40136879494066 28.63207138525513 29.008923156891626 14-15 23.187213029541713 22.57212163215802 23.771983020012126 30.46868231828814 16-17 24.642640561379192 22.806029628346185 25.42233388200641 27.128995928268214 18-19 21.944035346097202 22.117300528458806 27.999653469635277 27.939010655808715 20-21 24.1791561985619 22.338213635969854 26.630858528978603 26.851771636489648 22-23 24.101186866499177 22.307892229056574 26.565884085593 27.02503681885125 24-25 22.411851338473536 24.421727453868144 23.698345317508444 29.468075890149876 26-27 23.04426925409339 23.741661613098845 26.267001646019235 26.94706748678853 28-29 22.515810447890498 24.530018192844146 25.946461058650268 27.00771030061509 30-31 22.957636662912588 23.927921684137573 25.95945594732739 27.154985705622455 32-33 22.316555488174654 25.02815559213376 25.296716624794247 27.358572294897343 34-35 22.463830893182017 24.109850125617257 25.838170319674266 27.588148661526468 36-37 23.780646279130206 21.78809668197176 29.481070778826997 24.95018626007104 38-39 23.54673828294204 20.778826994715413 32.62150220913107 23.05293251321147 40-41 23.03560599497531 22.970631551589708 25.0108290738976 28.982933379537386 42-43 21.285627653123104 23.84995235207485 25.833838690115225 29.030581304686827 44-45 21.372260244303906 22.771376591873864 27.61847006843975 28.237893095382482 46-47 23.676687169713244 22.758381703196743 27.055358225764532 26.50957290132548 48-49 23.590054578532442 22.515810447890498 27.939010655808715 25.955124317768348 50-51 25.569609287013773 21.16867365502902 29.65000433162956 23.611712726327642 52-53 20.488607814259723 24.148834791648618 27.531837477258946 27.830719916832713 54-55 23.55106991250108 22.66308585289786 26.817118600017327 26.96872563458373 56-57 22.216928008316728 22.888330589967946 26.98605215281989 27.908689248895435 58-59 20.549250628086284 23.83695746339773 27.410551849605824 28.20324005891016 60-61 20.856796326778134 23.295503768517715 26.643853417655723 29.20384648704843 62-63 20.622888330589966 24.140171532530537 26.630858528978603 28.60608160790089 64-65 21.03872476825782 24.646972190938232 26.405613791908518 27.908689248895435 66-67 19.752230789222907 25.543619509659532 23.338820064108116 31.36532963700944 68-69 20.077972709551656 26.250812215724494 23.09291747888239 30.57829759584146 70-71 20.10396361273554 26.263807667316442 22.967294780160277 30.66493393978774 72-73 20.055444858355713 25.625920471281294 21.83141297756216 32.48722169280083 74-75 20.70952092177077 25.790522394524825 23.992896127523174 29.507060556181237 76 21.814086459326 24.118513384735337 24.343758121805422 29.723642034133242 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 1.0 19 1.5 20 7.0 21 12.0 22 12.5 23 15.5 24 21.5 25 25.0 26 33.5 27 45.0 28 53.5 29 59.0 30 65.0 31 102.5 32 149.5 33 165.0 34 191.5 35 254.0 36 300.0 37 310.0 38 343.5 39 412.0 40 480.0 41 538.0 42 563.0 43 592.0 44 609.0 45 585.5 46 574.0 47 564.5 48 564.0 49 559.0 50 545.0 51 514.0 52 433.5 53 373.5 54 363.0 55 337.5 56 291.0 57 245.0 58 220.0 59 219.0 60 201.0 61 198.0 62 212.0 63 208.0 64 203.5 65 200.0 66 203.5 67 210.0 68 205.5 69 208.5 70 206.0 71 196.0 72 186.5 73 157.0 74 122.0 75 107.0 76 97.5 77 73.0 78 52.0 79 46.0 80 36.0 81 24.5 82 17.5 83 12.0 84 11.0 85 8.0 86 3.0 87 0.0 88 0.5 89 1.0 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.004331629559040111 70-71 0.004331629559040111 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 11543.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.47526639521789 #Duplication Level Percentage of deduplicated Percentage of total 1 98.65399841646872 97.1497877501516 2 1.187648456057007 2.33907996188166 3 0.12316354359109703 0.3638568829593693 4 0.026392187912377938 0.10395910941696267 5 0.008797395970792645 0.04331629559040111 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.04331629559040111 0.0 20 0.0 0.0 0.0 0.04331629559040111 0.0 21 0.0 0.0 0.0 0.04331629559040111 0.0 22 0.0 0.0 0.0 0.08663259118080222 0.0 23 0.0 0.0 0.0 0.10395910941696267 0.0 24 0.0 0.0 0.0 0.1212856276531231 0.0 25 0.0 0.0 0.0 0.1212856276531231 0.0 26 0.0 0.0 0.0 0.1212856276531231 0.0 27 0.0 0.0 0.0 0.1212856276531231 0.0 28 0.0 0.0 0.0 0.1212856276531231 0.0 29 0.0 0.0 0.0 0.1212856276531231 0.0 30 0.0 0.0 0.0 0.155938664125444 0.0 31 0.0 0.0 0.0 0.155938664125444 0.0 32 0.0 0.0 0.0 0.17326518236160443 0.0 33 0.0 0.0 0.0 0.17326518236160443 0.0 34 0.0 0.0 0.0 0.17326518236160443 0.0 35 0.0 0.0 0.0 0.19059170059776487 0.0 36 0.0 0.0 0.0 0.20791821883392533 0.0 37 0.0 0.0 0.0 0.30321406913280774 0.0 38 0.0 0.0 0.0 0.39850991943169023 0.0 39 0.0 0.0 0.0 0.5197955470848133 0.0 40 0.0 0.0 0.0 0.6324179156198562 0.0 41 0.0 0.0 0.0 0.8316728753357013 0.0 42 0.0 0.0 0.0 0.9876115394611453 0.0 43 0.0 0.0 0.0 1.134886944468509 0.0 44 0.0 0.0 0.0 1.3428051633024343 0.0 45 0.0 0.0 0.0 1.4640907909555574 0.0 46 0.0 0.0 0.0 1.6286927141990817 0.008663259118080222 47 0.0 0.0 0.0 1.879927228623408 0.008663259118080222 48 0.0 0.0 0.0 2.044529151866932 0.008663259118080222 49 0.0 0.0 0.0 2.1311617430477345 0.008663259118080222 50 0.0 0.0 0.0 2.3737329983539808 0.008663259118080222 51 0.0 0.0 0.0 2.572987958069826 0.008663259118080222 52 0.0 0.0 0.0 2.8068959542579917 0.008663259118080222 53 0.0 0.0 0.0 3.162089578099281 0.008663259118080222 54 0.0 0.0 0.0 3.50861994282249 0.008663259118080222 55 0.0 0.0 0.0 3.6645586069479337 0.008663259118080222 56 0.0 0.0 0.0 3.863813566663779 0.008663259118080222 57 0.0 0.0 0.0 4.115048081088105 0.008663259118080222 58 0.0 0.0 0.0 4.28831326344971 0.008663259118080222 59 0.0 0.0 0.0 4.478904964047475 0.008663259118080222 60 0.0 0.0 0.0 4.548211036992116 0.008663259118080222 61 0.0 0.0 0.0 4.6868231828814 0.008663259118080222 62 0.0 0.0 0.0 4.808108810534523 0.008663259118080222 63 0.0 0.0 0.0 5.007363770250368 0.008663259118080222 64 0.0 0.0 0.0 5.215281989084294 0.008663259118080222 >>END_MODULE >>Kmer Content pass >>END_MODULE