FastQCFastQC Report
Wed 25 May 2016
SRR1779691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11543
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT6255.414536948800139No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT2632.2784371480550982Illumina PCR Primer Index 9 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1321.1435502035865892No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1000.8663259118080222No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG980.8489993935718617No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC810.701723988564498No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT410.3551936238412891Illumina PCR Primer Index 9 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG410.3551936238412891No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC380.3292038464870484No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC370.3205405873689682No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT300.2598977735424066No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT290.2512345144243264No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG280.2425712553062462No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT160.13861214588928356No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC150.1299488867712033No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT140.1212856276531231No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT140.1212856276531231No Hit
ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT120.10395910941696267Illumina PCR Primer Index 9 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAT150.00216916370.0000115
TTGAATG150.00216916370.0000116
CCGATCT150.00216916370.0000144
GGTCGAA150.00216916370.0000127
CTTTACA352.7235183E-760.01
TTACACA352.7235183E-760.03
CGTCAGA200.006779182252.52
GTCGAAG200.006779182252.528
TCAGATG200.006779182252.54
AGATGTG200.006779182252.56
CAGATGT200.006779182252.55
TTTACAC406.8519876E-752.52
TGTATAA200.006779182252.511
GTCAGAT200.006779182252.53
CTTATAC2000.028.0000021
GCTTGAA1900.027.63157856
TTGAAAA1900.027.63157858
CTTGAAA1900.027.63157857
TTCTGCT2050.027.31707452
TTATACA2050.027.3170742
Produced by FastQC (version 0.11.5)