##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779691_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11543
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00398509919432	34.0	34.0	34.0	31.0	34.0
2	33.27973663692281	34.0	34.0	34.0	33.0	34.0
3	33.37208697912155	34.0	34.0	34.0	34.0	34.0
4	33.43091050853331	34.0	34.0	34.0	34.0	34.0
5	33.350082300961624	34.0	34.0	34.0	34.0	34.0
6	36.60131681538595	38.0	37.0	38.0	34.0	38.0
7	36.69704582864073	38.0	37.0	38.0	34.0	38.0
8	36.34063934852291	38.0	37.0	38.0	34.0	38.0
9	36.30676600537122	38.0	37.0	38.0	34.0	38.0
10-11	36.28328857316123	38.0	37.0	38.0	34.0	38.0
12-13	36.30174131508274	38.0	37.0	38.0	34.0	38.0
14-15	36.35224811574115	38.0	37.0	38.0	34.0	38.0
16-17	36.1259204712813	38.0	36.5	38.0	32.5	38.0
18-19	36.190764965780126	38.0	37.0	38.0	34.0	38.0
20-21	36.29143203673222	38.0	37.0	38.0	34.0	38.0
22-23	36.12067919951485	38.0	37.0	38.0	33.0	38.0
24-25	36.03638568829594	38.0	36.0	38.0	32.5	38.0
26-27	35.892792168413756	38.0	36.0	38.0	31.5	38.0
28-29	35.781339339859656	38.0	36.0	38.0	31.0	38.0
30-31	35.75781859135407	38.0	36.0	38.0	31.0	38.0
32-33	35.764445984579396	38.0	36.0	38.0	31.0	38.0
34-35	35.57619336394352	38.0	36.0	38.0	31.0	38.0
36-37	35.47106471454561	38.0	36.0	38.0	31.0	38.0
38-39	35.065494238932686	38.0	35.5	38.0	27.0	38.0
40-41	35.52477692107771	38.0	36.0	38.0	31.0	38.0
42-43	35.78567096941869	38.0	36.0	38.0	31.5	38.0
44-45	35.73936584943256	38.0	36.0	38.0	31.0	38.0
46-47	35.797236420341335	38.0	36.0	38.0	31.0	38.0
48-49	35.74659967079615	38.0	36.0	38.0	31.5	38.0
50-51	35.64775188425886	38.0	36.0	38.0	31.0	38.0
52-53	35.745256865632854	38.0	36.0	38.0	31.0	38.0
54-55	35.723771983020015	38.0	36.0	38.0	31.0	38.0
56-57	35.64523953911461	38.0	36.0	38.0	31.0	38.0
58-59	35.53898466603136	38.0	36.0	38.0	31.0	38.0
60-61	35.60400242571255	38.0	36.0	38.0	31.0	38.0
62-63	35.57779606688036	38.0	36.0	38.0	31.0	38.0
64-65	35.633457506714024	38.0	36.0	38.0	31.0	38.0
66-67	35.59001126223686	38.0	36.0	38.0	31.0	38.0
68-69	35.07350775361691	38.0	36.0	38.0	27.5	38.0
70-71	34.03967772676081	37.5	34.5	38.0	23.5	38.0
72-73	32.884908602616306	37.0	33.5	38.0	15.0	38.0
74-75	32.77592480291085	37.5	34.0	38.0	11.0	38.0
76	31.839643073724336	37.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	4.0
24	8.0
25	12.0
26	41.0
27	59.0
28	92.0
29	162.0
30	251.0
31	377.0
32	567.0
33	847.0
34	1377.0
35	1968.0
36	2388.0
37	3389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.85350428831327	19.232435242138095	11.989950619423027	31.924109850125614
2	19.37971064714546	18.201507407086545	39.7730226111063	22.6457593346617
3	18.7472927315256	22.793034739669064	35.48470934765659	22.97496318114875
4	14.008489993935719	21.536862167547433	35.32010742441307	29.13454041410379
5	14.025816512171879	36.26440266828381	34.89560772762713	14.81417309191718
6	27.869704582864074	37.37329983539808	16.581477952005542	18.175517629732305
7	24.97617603742528	30.607294464177425	20.453954777787402	23.962574720609894
8	22.62843281642554	37.93641167807329	18.366109330330072	21.0690461751711
9	22.437841115827776	18.253486961795026	18.045568742961102	41.26310317941609
10-11	23.810967686043487	25.729879580698256	27.00771030061509	23.45144243264316
12-13	24.538681451962226	18.587022437841117	27.081348003118773	29.792948107077883
14-15	24.772589448150395	19.84319500996275	20.518929221173003	34.86528632071385
16-17	28.1425972450836	27.090011262236853	19.297409685523697	25.46998180715585
18-19	27.449536515637185	23.84995235207485	20.544918998527244	28.15559213376072
20-21	23.5857229489734	22.381529931560255	20.518929221173003	33.51381789829333
22-23	25.01949233301568	27.154985705622455	19.84319500996275	27.982326951399116
24-25	28.289872650090963	27.869704582864074	20.30667937278004	23.53374339426492
26-27	28.57576020098761	27.874036212423114	22.320887117733694	21.229316468855583
28-29	27.289266221952698	23.728666724421725	24.469375379017585	24.512691674607986
30-31	23.992896127523174	23.949579831932773	29.896907216494846	22.160616824049207
32-33	23.08758554968379	32.0194057004245	23.174218140864593	21.718790609027117
34-35	23.823962574720607	29.827601143550204	22.022004678159924	24.32643160356926
36-37	26.91241445031621	27.393225331369663	22.788703110110024	22.905657108204107
38-39	22.99228969938491	22.520142077449538	27.401888590487744	27.085679632677813
40-41	23.702676947067484	20.796153512951573	28.350515463917525	27.150654076063415
42-43	27.835051546391753	21.944035346097202	28.19024517023304	22.030667937278004
44-45	23.681018799272284	25.39201247509313	27.969332062721996	22.957636662912588
46-47	27.73975569609287	20.882786104132375	24.495365156371825	26.88209304340293
48-49	26.92540933899333	19.84319500996275	24.465043749458545	28.766351901585374
50-51	23.51641687602876	19.804210343931388	28.995928268214506	27.68344451182535
52-53	22.463830893182017	19.94282248982067	33.66542493285974	23.927921684137573
54-55	23.10491206791995	20.38464870484276	30.455687429611018	26.05475179762627
56-57	26.838776747812528	19.84752663952179	26.53989430823876	26.773802304426926
58-59	23.12223858615611	22.64142770510266	31.75517629732305	22.481157411418177
60-61	26.154379277484193	29.55904011088972	23.395131248375638	20.891449363250455
62-63	20.596898553235725	36.563285107857574	21.91804556874296	20.921770770163736
64-65	20.696526033093647	37.31698865113056	21.16434202546998	20.822143290305814
66-67	20.488607814259723	37.130728580091834	21.08204106384822	21.298622541800224
68-69	20.168067226890756	37.5379017586416	20.804816772069653	21.489214242397992
70-71	21.203326691501342	35.01256172572121	21.575846833578794	22.208264749198648
72-73	21.718790609027117	32.17534436454994	21.701464090790957	24.404400935631983
74-75	20.891449363250455	30.728580091830548	23.382136359698517	24.99783418522048
76	21.623494758728235	30.711253573594387	22.689075630252102	24.97617603742528
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	2.0
16	1.0
17	1.0
18	2.0
19	5.5
20	9.0
21	10.0
22	12.5
23	17.5
24	25.0
25	30.0
26	39.0
27	44.5
28	59.5
29	78.0
30	89.0
31	103.0
32	140.0
33	174.0
34	172.0
35	207.5
36	265.0
37	285.0
38	345.5
39	668.0
40	957.5
41	922.5
42	860.0
43	764.0
44	652.5
45	576.5
46	516.0
47	517.0
48	474.0
49	400.5
50	371.0
51	335.5
52	269.5
53	234.5
54	230.0
55	230.5
56	215.0
57	184.5
58	170.0
59	157.5
60	133.0
61	138.5
62	156.0
63	157.0
64	166.0
65	169.5
66	178.5
67	192.0
68	185.0
69	185.5
70	187.0
71	181.0
72	179.5
73	170.0
74	144.0
75	126.0
76	121.0
77	96.5
78	70.0
79	63.0
80	53.0
81	40.0
82	28.5
83	20.0
84	19.5
85	13.0
86	4.0
87	1.0
88	1.0
89	0.5
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	11543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.55609460278957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84124134393434	54.613185480377716
2	13.593229033085407	18.366109330330072
3	3.475250064119005	7.04322966299922
4	1.1541420877147985	3.11877328250888
5	0.3847140292382662	1.2994888677120333
6	0.07694280584765324	0.311877328250888
7	0.14106181072069762	0.667070952092177
8	0.06411900487304437	0.34653036472320886
9	0.025647601949217745	0.155938664125444
>10	0.16670941266991535	2.8242224724941525
>50	0.03847140292382662	2.417049293944382
>100	0.025647601949217745	3.421987351641688
>500	0.012823800974608873	5.414536948800139
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	625	5.414536948800139	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT	263	2.2784371480550982	Illumina PCR Primer Index 9 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	132	1.1435502035865892	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	100	0.8663259118080222	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	98	0.8489993935718617	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	81	0.701723988564498	No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT	41	0.3551936238412891	Illumina PCR Primer Index 9 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	41	0.3551936238412891	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	38	0.3292038464870484	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	37	0.3205405873689682	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT	30	0.2598977735424066	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT	29	0.2512345144243264	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	28	0.2425712553062462	No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	16	0.13861214588928356	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	15	0.1299488867712033	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT	14	0.1212856276531231	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT	14	0.1212856276531231	No Hit
ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT	12	0.10395910941696267	Illumina PCR Primer Index 9 (95% over 24bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.008663259118080222	0.0
19	0.0	0.0	0.0	0.05197955470848133	0.0
20	0.0	0.0	0.0	0.05197955470848133	0.0
21	0.0	0.0	0.0	0.06064281382656155	0.0
22	0.0	0.0	0.0	0.06064281382656155	0.0
23	0.0	0.0	0.0	0.06930607294464178	0.0
24	0.0	0.0	0.0	0.06930607294464178	0.0
25	0.0	0.0	0.0	0.06930607294464178	0.0
26	0.0	0.0	0.0	0.06930607294464178	0.0
27	0.0	0.0	0.0	0.06930607294464178	0.0
28	0.0	0.0	0.0	0.10395910941696267	0.0
29	0.0	0.0	0.0	0.10395910941696267	0.0
30	0.0	0.0	0.0	0.12994888677120334	0.0
31	0.0	0.0	0.0	0.155938664125444	0.0
32	0.0	0.0	0.0	0.18192844147968465	0.0
33	0.0	0.0	0.0	0.19059170059776487	0.0
34	0.0	0.0	0.0	0.19925495971584511	0.0
35	0.0	0.0	0.0	0.233907996188166	0.0
36	0.0	0.0	0.0	0.30321406913280774	0.0
37	0.0	0.0	0.0	0.4851425106124924	0.0
38	0.0	0.0	0.0	0.6324179156198562	0.0
39	0.0	0.0	0.0	0.7883565797453002	0.0
40	0.0	0.0	0.0	0.9529585029888243	0.0
41	0.0	0.0	0.0	1.1782032400589102	0.0
42	0.0	0.0	0.0	1.3774581997747553	0.0
43	0.0	0.0	0.0	1.5507233821363597	0.0
44	0.0	0.0	0.0	1.8192844147968465	0.0
45	0.0	0.0	0.0	2.0185393745126916	0.0
46	0.0	0.0	0.0	2.261110629818938	0.0
47	0.0	0.0	0.0	2.5296716624794247	0.0
48	0.0	0.0	0.0	2.8242224724941525	0.0
49	0.0	0.0	0.0	3.0927835051546393	0.0
50	0.0	0.0	0.0	3.3700077969332063	0.0
51	0.0	0.0	0.0	3.595252534003292	0.0
52	0.0	0.0	0.0	3.91579312137226	0.0
53	0.0	0.0	0.0	4.219007190505068	0.0
54	0.0	0.0	0.0	4.548211036992116	0.0
55	0.0	0.0	0.0	4.7907822922983625	0.0
56	0.0	0.0	0.0	5.050680065840769	0.0
57	0.0	0.0	0.0	5.431863467036299	0.0
58	0.0	0.0	0.0	5.709087758814866	0.0
59	0.0	0.0	0.0	5.960322273239193	0.0
60	0.0	0.0	0.0	6.289526119726241	0.0
61	0.0	0.0	0.0	6.532097375032487	0.0
62	0.0	0.0	0.0	6.800658407692974	0.0
63	0.0	0.0	0.0	7.1558520315342635	0.0
64	0.0	0.0	0.0	7.545698691847873	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAT	15	0.002169163	70.00001	15
TTGAATG	15	0.002169163	70.00001	16
CCGATCT	15	0.002169163	70.00001	44
GGTCGAA	15	0.002169163	70.00001	27
CTTTACA	35	2.7235183E-7	60.0	1
TTACACA	35	2.7235183E-7	60.0	3
CGTCAGA	20	0.0067791822	52.5	2
GTCGAAG	20	0.0067791822	52.5	28
TCAGATG	20	0.0067791822	52.5	4
AGATGTG	20	0.0067791822	52.5	6
CAGATGT	20	0.0067791822	52.5	5
TTTACAC	40	6.8519876E-7	52.5	2
TGTATAA	20	0.0067791822	52.5	11
GTCAGAT	20	0.0067791822	52.5	3
CTTATAC	200	0.0	28.000002	1
GCTTGAA	190	0.0	27.631578	56
TTGAAAA	190	0.0	27.631578	58
CTTGAAA	190	0.0	27.631578	57
TTCTGCT	205	0.0	27.317074	52
TTATACA	205	0.0	27.317074	2
>>END_MODULE