FastQCFastQC Report
Wed 25 May 2016
SRR1779690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91981
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT28113.0560659266587664No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG3790.4120416172905274No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT3650.3968210826148878RNA PCR Primer, Index 42 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1790.19460540763853407RNA PCR Primer, Index 32 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1640.17829769191463454No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1590.1728617866733347No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1560.16960024352855482No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT1160.12611300159815614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGA150.002218382470.0000121
AAGTATT200.006931815852.515
GCAAAGT200.006931815852.553
ACCCATA200.006931815852.58
TTCCTTA200.006931815852.519
CTCTAGC305.8510725E-446.6666682
AACCAGT350.001249754240.024
TCAATTA350.001249754240.028
TTTATAC350.001249754240.03
ATGCCGT5750.038.9565244
CTTTATA459.13602E-538.8888852
CTCGTAT5700.038.6842139
GTCTTCT5700.038.6842149
GTATGCC5800.038.6206942
TCGTATG5850.038.290640
CCGTCTT5700.038.07017547
GCCGTCT5800.038.01724246
TCTCGTA5250.038.038
CGTATGC5900.037.966141
TATGCCG5900.037.966143