##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779690_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	91981
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.21908872484535	34.0	34.0	34.0	32.0	34.0
2	33.3571389743534	34.0	34.0	34.0	33.0	34.0
3	33.40942151096422	34.0	34.0	34.0	34.0	34.0
4	33.46563964296974	34.0	34.0	34.0	34.0	34.0
5	33.43822093693263	34.0	34.0	34.0	34.0	34.0
6	36.742077168110804	38.0	37.0	38.0	34.0	38.0
7	36.803839923462455	38.0	37.0	38.0	34.0	38.0
8	36.51179047846838	38.0	37.0	38.0	34.0	38.0
9	36.57439036322719	38.0	37.0	38.0	34.0	38.0
10-11	36.433464519846495	38.0	37.0	38.0	34.0	38.0
12-13	36.47935443189354	38.0	37.0	38.0	34.0	38.0
14-15	36.537475130733526	38.0	37.0	38.0	34.0	38.0
16-17	36.43070307998391	38.0	37.0	38.0	34.0	38.0
18-19	36.45993194246638	38.0	37.0	38.0	34.0	38.0
20-21	36.49411291462367	38.0	37.0	38.0	34.0	38.0
22-23	36.321571846359575	38.0	37.0	38.0	34.0	38.0
24-25	36.32660549461302	38.0	37.0	38.0	34.0	38.0
26-27	36.187332166425676	38.0	37.0	38.0	34.0	38.0
28-29	36.06909579152216	38.0	37.0	38.0	33.0	38.0
30-31	35.978930431284724	38.0	37.0	38.0	32.0	38.0
32-33	35.98317587327818	38.0	37.0	38.0	32.0	38.0
34-35	35.923957121579456	38.0	37.0	38.0	32.0	38.0
36-37	36.050521303312635	38.0	37.0	38.0	32.5	38.0
38-39	35.8462671638708	38.0	37.0	38.0	32.0	38.0
40-41	36.02832106630717	38.0	37.0	38.0	32.5	38.0
42-43	36.08282145225644	38.0	37.0	38.0	33.5	38.0
44-45	36.07967949902697	38.0	37.0	38.0	33.0	38.0
46-47	36.11831247757689	38.0	37.0	38.0	33.5	38.0
48-49	36.09532403431143	38.0	37.0	38.0	33.5	38.0
50-51	36.06709537839336	38.0	37.0	38.0	33.0	38.0
52-53	36.0475152477142	38.0	37.0	38.0	33.0	38.0
54-55	36.05280438351399	38.0	37.0	38.0	33.0	38.0
56-57	36.061746447635926	38.0	37.0	38.0	33.0	38.0
58-59	36.01793848729629	38.0	37.0	38.0	32.5	38.0
60-61	36.05594633674346	38.0	37.0	38.0	33.0	38.0
62-63	35.99550450636545	38.0	37.0	38.0	32.0	38.0
64-65	35.965226514171405	38.0	37.0	38.0	32.0	38.0
66-67	35.95868712016612	38.0	37.0	38.0	32.0	38.0
68-69	35.832454528652654	38.0	36.0	38.0	31.0	38.0
70-71	35.50137528402605	38.0	36.0	38.0	30.0	38.0
72-73	35.08846392189692	38.0	36.0	38.0	27.0	38.0
74-75	35.080337243561175	38.0	36.0	38.0	27.0	38.0
76	34.12885269783977	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	5.0
22	19.0
23	20.0
24	58.0
25	84.0
26	186.0
27	302.0
28	557.0
29	934.0
30	1492.0
31	2338.0
32	3601.0
33	5266.0
34	8033.0
35	12028.0
36	18173.0
37	38885.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.58141355279895	19.138735173568456	10.940302888640046	28.339548384992554
2	21.893651949859212	19.336602124351767	34.07877713875692	24.690968787032105
3	21.033691740685576	21.28156901968885	29.806155619095247	27.87858362053033
4	16.123982126743567	18.856068101020863	33.07530903121297	31.9446407410226
5	16.175079636011784	33.70369967710723	34.13857209641121	15.98264859046977
6	32.59042628368902	35.17574281645122	16.447962079125038	15.785868820734716
7	30.0736021569672	27.587219099596656	19.508376729976842	22.8308020134593
8	26.140181124362638	35.30729172329068	17.609071438666682	20.94345571368
9	24.680096976549503	15.822832976375556	19.141996716713233	40.355073330361705
10-11	27.50024461573586	24.229460432045748	24.93341016079408	23.336884791424318
12-13	26.52286885335015	20.010110783748818	25.46884682706211	27.998173535838923
14-15	24.954610191235147	21.060327676367947	20.729824637696918	33.25523749469999
16-17	27.51437796936324	25.674867635707376	21.67349778758657	25.137256607342824
18-19	27.0985312184038	23.52659788434568	22.777530142094562	26.597340755155958
20-21	24.738804753155545	23.264587251715028	22.66120176993075	29.335406225198685
22-23	25.401985192594122	25.381328752677184	22.682945390895945	26.53374066383275
24-25	27.320859742772964	25.50744175427534	22.58455550602842	24.587142996923276
26-27	26.533197073308617	26.091801567715073	23.267305204335678	24.10769615464063
28-29	24.56159424228917	25.950468031441275	23.782085430686774	25.705852295582783
30-31	24.463204357421642	23.417879779519684	25.699872799817353	26.419043063241322
32-33	24.075624313716965	25.55310335830226	23.401572063795783	26.969700264184993
34-35	25.224774681727745	23.148802470075342	25.531359737337056	26.09506311085985
36-37	27.06537219643187	24.651286678770614	23.479849099270503	24.80349202552701
38-39	24.315891325382417	23.544536371641968	25.937965449386287	26.201606853589325
40-41	24.304475924375687	22.516063099988042	26.623976690838326	26.55548428479795
42-43	26.500038051336688	22.56335547558735	26.346745523532032	24.589860949543926
44-45	23.94081386373273	24.645850773529315	26.893597590806795	24.51973777193116
46-47	26.312499320511844	22.344832084887095	24.654548221915395	26.688120372685663
48-49	25.81293962883639	22.143703590959003	24.730650895293593	27.312705884911015
50-51	23.661951924854048	22.095867624835563	27.179526206499165	27.062654243811224
52-53	23.20261793196421	22.042052162946696	29.731683717289442	25.023646187799653
54-55	22.711212098150703	22.390493688914013	27.873691305813157	27.024602907122127
56-57	24.90623063458758	22.31873973972886	25.644970156880227	27.130059468803342
58-59	22.91125341103054	22.836781509224732	28.912492797425553	25.339472282319175
60-61	24.654004631391267	25.136169426294565	24.870353659994997	25.339472282319175
62-63	22.352985942749047	28.210717430773748	23.954403626835976	25.481892999641232
64-65	22.121959969993803	29.03425707483067	23.23795131603266	25.605831639142867
66-67	22.469857905436992	28.885313271219054	22.82210456507322	25.82272425827073
68-69	22.023570085126277	28.991857013948536	23.026494602146094	25.958078298779096
70-71	22.53182722518781	28.171035322512257	23.098792141855384	26.198345310444548
72-73	22.16164207825529	26.969156673660866	23.133581935399704	27.73561931268414
74-75	22.236113980061102	26.745740968243442	23.345582239810394	27.672562811885065
76	22.85906872071406	26.16409910742436	23.629880083930377	27.346952087931204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	3.0
16	9.5
17	13.0
18	14.5
19	21.5
20	35.5
21	44.0
22	60.5
23	104.0
24	185.0
25	239.0
26	257.0
27	318.0
28	429.5
29	498.0
30	589.5
31	758.0
32	1037.5
33	1240.0
34	1397.0
35	1693.0
36	1998.0
37	2164.0
38	2408.0
39	2949.5
40	4078.5
41	5180.0
42	5450.0
43	5326.5
44	5055.5
45	4686.0
46	4464.0
47	4317.0
48	3968.0
49	3661.5
50	3557.0
51	3298.5
52	2833.5
53	2535.0
54	2443.0
55	2348.0
56	2204.5
57	2066.0
58	1976.0
59	1974.0
60	1982.5
61	2020.0
62	2047.0
63	2062.0
64	2099.5
65	2082.0
66	2024.5
67	2007.0
68	1916.5
69	1751.0
70	1598.0
71	1520.0
72	1392.0
73	1209.5
74	1035.5
75	916.0
76	820.0
77	639.5
78	468.5
79	382.0
80	367.0
81	283.0
82	177.0
83	140.0
84	104.0
85	60.0
86	42.0
87	32.0
88	22.0
89	11.0
90	8.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	91981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.336841304182386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.50528072204631	39.27876409258434
2	18.838051536813364	21.60228742892554
3	6.334970325565521	10.896815646709648
4	2.398604448320977	5.501136104195432
5	1.2154193291492066	3.484415259673193
6	0.6920874495155388	2.3809264956893275
7	0.5517738296137583	2.214587795305552
8	0.3602646997478147	1.6525151933551494
9	0.23701624983408862	1.2230786792924626
>10	0.8399855894120101	6.650286472206217
>50	0.011376779992036254	0.4087800741457475
>100	0.013272909990708962	1.6503408312586294
>500	0.0	0.0
>1k	0.0018961299986727089	3.0560659266587664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	2811	3.0560659266587664	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	379	0.4120416172905274	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	365	0.3968210826148878	RNA PCR Primer, Index 42 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	179	0.19460540763853407	RNA PCR Primer, Index 32 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	164	0.17829769191463454	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	159	0.1728617866733347	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	156	0.16960024352855482	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	116	0.12611300159815614	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0021743620965199335	0.0
2	0.0	0.0	0.0	0.0021743620965199335	0.0
3	0.0	0.0	0.0	0.0021743620965199335	0.0
4	0.0	0.0	0.0	0.0021743620965199335	0.0
5	0.0	0.0	0.0	0.0021743620965199335	0.0
6	0.0	0.0	0.0	0.0021743620965199335	0.0
7	0.0	0.0	0.0	0.0021743620965199335	0.0
8	0.0	0.0	0.0	0.0021743620965199335	0.0
9	0.0	0.0	0.0	0.0021743620965199335	0.0
10	0.0	0.0	0.0	0.004348724193039867	0.0
11	0.0	0.0	0.0	0.005435905241299834	0.0
12	0.0	0.0	0.0	0.005435905241299834	0.0
13	0.0	0.0	0.0	0.007610267337819767	0.0
14	0.0	0.0	0.0	0.007610267337819767	0.0
15	0.0	0.0	0.0	0.007610267337819767	0.0
16	0.0	0.0	0.0	0.008697448386079734	0.0
17	0.0	0.0	0.0	0.009784629434339701	0.0
18	0.0	0.0	0.0	0.010871810482599668	0.0
19	0.0	0.0	0.0	0.018482077820419433	0.0
20	0.0	0.0	0.0	0.020656439916939367	0.0
21	0.0	0.0	0.0	0.020656439916939367	0.0
22	0.0	0.0	0.0	0.021743620965199335	0.0
23	0.0	0.0	0.0	0.02391798306171927	0.0
24	0.0	0.0	0.0	0.02391798306171927	0.0
25	0.0	0.0	0.0	0.026092345158239202	0.0
26	0.0	0.0	0.0	0.026092345158239202	0.0
27	0.0	0.0	0.0	0.039138517737358804	0.0
28	0.0	0.0	0.0	0.05762059555777824	0.0
29	0.0	0.0	0.0	0.07610267337819768	0.0
30	0.0	0.0	0.0	0.10110783748817691	0.0
31	0.0	0.0	0.0	0.13154890683945597	0.0
32	0.0	0.0	0.0	0.1663387003837749	0.0
33	0.0	0.0	0.0	0.21743620965199334	0.0
34	0.0	0.0	0.0	0.28049271045107144	0.0
35	0.0	0.0	0.0	0.3739902806014286	0.0
36	0.0	0.0	0.0	0.5479392483230232	0.0
37	0.0	0.0	0.0	0.8045139757123754	0.0
38	0.0	0.0	0.0	1.0882682293082266	0.0
39	0.0	0.0	0.0	1.376371207097118	0.0
40	0.0	0.0	0.0	1.7101357889129276	0.0
41	0.0	0.0	0.0	2.1417466650721346	0.0
42	0.0	0.0	0.0	2.6070601537274003	0.0
43	0.0	0.0	0.0	3.0897685391548255	0.0
44	0.0	0.0	0.0	3.5855230971613703	0.0
45	0.0	0.0	0.0	4.196518846283472	0.0
46	0.0	0.0	0.0	4.833606940563812	0.0
47	0.0	0.0	0.0	5.390243637272915	0.0
48	0.0	0.0	0.0	5.966449592850697	0.0
49	0.0	0.0	0.0	6.52634783270458	0.0
50	0.0	0.0	0.0	7.047107554821104	0.0
51	0.0	0.0	0.0	7.52981594024853	0.0
52	0.0	0.0	0.0	8.081016731716332	0.0
53	0.0	0.0	0.0	8.609386721170676	0.0
54	0.0	0.0	0.0	9.204074754568879	0.0
55	0.0	0.0	0.0	9.699829312575423	0.0
56	0.0	0.0	0.0	10.224937758884987	0.0
57	0.0	0.0	0.0	10.76526673987019	0.0
58	0.0	0.0	0.0	11.312118807144953	0.0
59	0.0	0.0	0.0	11.76547330426936	0.0
60	0.0	0.0	0.0	12.291668931627184	0.0
61	0.0	0.0	0.0	12.798295300116328	0.0
62	0.0	0.0	0.0	13.312531935943293	0.0
63	0.0	0.0	0.0	13.858296822169796	0.0
64	0.0	0.0	0.0	14.36927191485198	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGA	15	0.0022183824	70.00001	21
AAGTATT	20	0.0069318158	52.5	15
GCAAAGT	20	0.0069318158	52.5	53
ACCCATA	20	0.0069318158	52.5	8
TTCCTTA	20	0.0069318158	52.5	19
CTCTAGC	30	5.8510725E-4	46.666668	2
AACCAGT	35	0.0012497542	40.0	24
TCAATTA	35	0.0012497542	40.0	28
TTTATAC	35	0.0012497542	40.0	3
ATGCCGT	575	0.0	38.95652	44
CTTTATA	45	9.13602E-5	38.888885	2
CTCGTAT	570	0.0	38.68421	39
GTCTTCT	570	0.0	38.68421	49
GTATGCC	580	0.0	38.62069	42
TCGTATG	585	0.0	38.2906	40
CCGTCTT	570	0.0	38.070175	47
GCCGTCT	580	0.0	38.017242	46
TCTCGTA	525	0.0	38.0	38
CGTATGC	590	0.0	37.9661	41
TATGCCG	590	0.0	37.9661	43
>>END_MODULE