##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779689_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348764
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56179536878806	34.0	32.0	34.0	23.0	34.0
2	29.43554667339519	34.0	27.0	34.0	12.0	34.0
3	28.319958481953414	34.0	23.0	34.0	11.0	34.0
4	31.39566870433875	34.0	32.0	34.0	23.0	34.0
5	30.71086465346194	34.0	32.0	34.0	21.0	34.0
6	33.53026115080685	37.0	34.0	38.0	21.0	38.0
7	34.659629434230595	38.0	35.0	38.0	26.0	38.0
8	33.86465059467147	38.0	34.0	38.0	21.0	38.0
9	35.254381186131596	38.0	36.0	38.0	28.0	38.0
10-11	35.18541907995091	38.0	36.5	38.0	27.0	38.0
12-13	33.80979974997419	37.5	32.5	38.0	21.0	38.0
14-15	34.21617770182702	37.5	35.0	38.0	24.0	38.0
16-17	33.77941387299148	37.5	34.0	38.0	21.0	38.0
18-19	34.397457879827044	38.0	34.0	38.0	25.0	38.0
20-21	33.34850070534803	37.0	31.0	38.0	19.0	38.0
22-23	33.22084274753128	37.5	31.5	38.0	19.0	38.0
24-25	34.83388337099012	37.5	35.0	38.0	28.5	38.0
26-27	33.09289806287346	37.0	32.5	38.0	17.5	38.0
28-29	35.571300363569634	38.0	36.5	38.0	31.0	38.0
30-31	35.98047963665975	38.0	37.0	38.0	33.0	38.0
32-33	36.04768410730465	38.0	37.0	38.0	34.0	38.0
34-35	36.09794015437373	38.0	37.0	38.0	34.0	38.0
36-37	33.71682140358523	38.0	33.5	38.0	19.0	38.0
38-39	31.588009370233166	36.5	28.5	38.0	11.0	38.0
40-41	33.28031992980927	37.0	32.5	38.0	18.0	38.0
42-43	34.43356682455758	37.5	35.0	38.0	26.0	38.0
44-45	36.09513739950224	38.0	37.0	38.0	34.0	38.0
46-47	34.479176463167065	38.0	34.5	38.0	22.5	38.0
48-49	33.975157986489435	37.5	33.5	38.0	21.0	38.0
50-51	31.512271908797928	36.5	27.5	38.0	11.0	38.0
52-53	35.404454014749234	37.5	35.5	38.0	30.0	38.0
54-55	34.37406096959549	37.5	35.5	38.0	21.0	38.0
56-57	34.740067782225225	38.0	35.5	38.0	27.5	38.0
58-59	35.973275624777784	38.0	37.0	38.0	33.5	38.0
60-61	36.31082479843103	38.0	37.0	38.0	34.0	38.0
62-63	36.20006652062713	38.0	37.0	38.0	34.0	38.0
64-65	35.87794181738941	38.0	37.5	38.0	30.5	38.0
66-67	33.66723343005586	37.5	34.0	38.0	21.5	38.0
68-69	33.77366786709638	37.5	34.0	38.0	22.5	38.0
70-71	33.15623458843229	37.0	32.5	38.0	16.5	38.0
72-73	31.892164902340838	37.0	29.0	38.0	11.0	38.0
74-75	34.550709075477975	37.5	35.5	38.0	26.5	38.0
76	35.37634904978725	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	17.0
13	119.0
14	358.0
15	626.0
16	934.0
17	1322.0
18	1645.0
19	1806.0
20	1727.0
21	1669.0
22	1671.0
23	1848.0
24	2133.0
25	2678.0
26	3229.0
27	4134.0
28	5236.0
29	6844.0
30	9166.0
31	12533.0
32	18687.0
33	28104.0
34	44157.0
35	66354.0
36	83873.0
37	47892.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.663004209150024	22.666616967347547	13.778084894083104	25.892293929419317
2	19.401371701207694	21.096214058790473	38.44662866580266	21.05578557419917
3	21.13291509444782	21.647303047332866	33.739434115906455	23.480347742312855
4	14.016354899014807	19.024325905196637	36.35352272596942	30.605796469819136
5	13.988255668589646	33.22906033879644	37.732105377848626	15.050578614765286
6	27.656810909382852	33.82029108508905	18.571010769460152	19.951887236067943
7	24.667683591196337	30.43433381885745	23.770515305478774	21.127467284467432
8	21.53662648667867	33.3368696310399	21.91223864848436	23.214265233797065
9	22.950476540009863	17.56746682570449	23.929361975433245	35.5526946588524
10-11	23.695536236538175	26.487968941748573	28.376208553635124	21.440286268078125
12-13	23.845924464680987	23.36092601300593	29.53845580392472	23.254693718388367
14-15	21.45720315170144	25.848711449576218	26.10748242364464	26.586602975077707
16-17	21.14280717046484	28.12145175534172	28.667809751006413	22.067931323187025
18-19	20.57164730304733	27.731933341743986	29.410718996226677	22.285700358982005
20-21	21.552253099517152	26.9579142342673	29.622753495200193	21.86707917101536
22-23	21.063527198908144	26.593197692422383	30.390894702434885	21.95238040623459
24-25	21.343946049477584	27.73695106146277	27.4089355552752	23.51016733378445
26-27	20.267573488089365	26.319230195777088	30.41125230815107	23.001944007982473
28-29	20.901813260542944	28.22080260577353	28.429396382654172	22.44798775102935
30-31	21.435125184938812	28.38739090043697	27.89837827298689	22.279105641637326
32-33	21.244881925886848	28.063819660286036	28.143529722104343	22.54776869172277
34-35	21.171623217992682	27.691791583993762	28.391835166473605	22.744750031539954
36-37	20.69967083758645	27.040921654757945	31.220538817079746	21.038868690575864
38-39	21.011199550412314	25.856309710864657	32.911940452569645	20.220550286153387
40-41	21.114994666880758	25.56241469876478	27.72462180729662	25.597968827057837
42-43	20.494087692537075	26.102751430766936	27.991851223176706	25.411309653519286
44-45	20.90396371185099	27.262704866327947	28.94249406475439	22.89083735706667
46-47	20.77809062861993	26.658428048766503	30.733676640937713	21.82980468167586
48-49	20.900522989758116	26.697279535731898	30.4710348545148	21.931162619995185
50-51	20.03862210549254	25.393532589372757	33.519944719065045	21.047900586069662
52-53	20.080054134027595	27.450797674071865	29.514084022433508	22.955064169467033
54-55	20.551433060751684	27.14199286623619	29.867761580897113	22.438812492115012
56-57	20.66856097533002	26.920209654666195	29.50892293929419	22.90230643070959
58-59	19.746447454439107	27.407071830808228	29.65099608904589	23.19548462570678
60-61	20.134102143569862	27.300696172770127	29.26004404124279	23.30515764241722
62-63	20.13180832884128	27.55444942711977	29.142342672982302	23.171399571056643
64-65	20.314309963184275	27.346429103921277	28.76845087222305	23.5708100606714
66-67	19.555630741704995	24.583099173079788	27.164357559839893	28.696912525375325
68-69	19.871957558160375	25.209305660106825	27.51464315159336	27.404093630139442
70-71	19.820136252594878	24.21479854572146	27.230878186968837	28.734187014714824
72-73	19.608818570724043	23.07563280613825	26.551622300466793	30.76392632267092
74-75	20.977652510006767	27.253099517152002	27.350299916275763	24.41894805656547
76	21.21778623940544	27.6201098737255	27.338830842632838	23.823273044236217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	1.0
7	1.5
8	2.0
9	1.0
10	4.0
11	12.0
12	16.0
13	18.0
14	24.5
15	53.5
16	114.0
17	150.0
18	245.5
19	432.0
20	717.0
21	911.0
22	1151.0
23	1720.0
24	2429.0
25	2809.0
26	3296.0
27	4401.0
28	5630.0
29	6241.0
30	6897.5
31	8350.0
32	9882.0
33	10618.0
34	11381.5
35	12927.0
36	14359.0
37	15009.0
38	15696.0
39	16752.0
40	17199.0
41	17441.0
42	17605.0
43	17497.5
44	17160.0
45	16592.0
46	16254.0
47	15782.5
48	14903.5
49	14033.5
50	13571.0
51	12913.5
52	11827.5
53	10848.0
54	10297.0
55	9942.0
56	9074.5
57	8041.0
58	7520.0
59	6888.0
60	5744.5
61	4732.0
62	4231.0
63	3797.5
64	3094.5
65	2606.5
66	2167.5
67	1947.0
68	1836.5
69	1559.5
70	1309.5
71	1226.0
72	1109.5
73	928.0
74	793.5
75	724.0
76	642.5
77	500.0
78	383.5
79	328.0
80	289.0
81	223.0
82	156.0
83	116.0
84	94.0
85	56.0
86	39.0
87	38.0
88	27.5
89	13.0
90	5.5
91	3.0
92	4.0
93	4.0
94	2.5
95	0.5
96	0.0
97	0.0
98	1.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.014766432315261896
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	348764.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.46614782146162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.58958097833678	97.07736254268872
2	1.2827474083377695	2.526143918539672
3	0.11111530263512134	0.3282328744348891
4	0.0140988937962081	0.0555305504262606
5	0.0021367507734528475	0.010519880875821527
6	0.0	0.0
7	3.2066612065622795E-4	0.002210233034650955
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8672684107304656E-4	0.0
3	0.0	0.0	0.0	2.8672684107304656E-4	0.0
4	0.0	0.0	0.0	5.734536821460931E-4	0.0
5	0.0	0.0	0.0	8.601805232191396E-4	0.0
6	0.0	0.0	0.0	8.601805232191396E-4	0.0
7	0.0	0.0	0.0	8.601805232191396E-4	0.0
8	0.0	0.0	0.0	8.601805232191396E-4	0.0
9	0.0	0.0	0.0	0.002580541569657419	0.0
10	0.0	0.0	0.0	0.002580541569657419	0.0
11	0.0	0.0	0.0	0.0028672684107304653	0.0
12	0.0	0.0	0.0	0.003153995251803512	0.0
13	0.0	0.0	0.0	0.003153995251803512	0.0
14	0.0	0.0	0.0	0.0034407220928765582	0.0
15	0.0	0.0	0.0	0.0034407220928765582	2.8672684107304656E-4
16	0.0	0.0	0.0	0.0037274489339496047	2.8672684107304656E-4
17	0.0	0.0	0.0	0.004014175775022651	2.8672684107304656E-4
18	0.0	0.0	0.0	0.004300902616095698	2.8672684107304656E-4
19	0.0	0.0	0.0	0.004587629457168745	2.8672684107304656E-4
20	0.0	0.0	0.0	0.005161083139314838	2.8672684107304656E-4
21	0.0	0.0	0.0	0.005734536821460931	2.8672684107304656E-4
22	0.0	0.0	0.0	0.007454897867899209	2.8672684107304656E-4
23	0.0	0.0	0.0	0.00831507839111835	2.8672684107304656E-4
24	0.0	0.0	0.0	0.010322166278629676	2.8672684107304656E-4
25	0.0	0.0	0.0	0.011182346801848814	2.8672684107304656E-4
26	0.0	0.0	0.0	0.012615981007214047	2.8672684107304656E-4
27	0.0	0.0	0.0	0.016343429941163654	2.8672684107304656E-4
28	0.0	0.0	0.0	0.023511600967989814	2.8672684107304656E-4
29	0.0	0.0	0.0	0.0289594109483777	2.8672684107304656E-4
30	0.0	0.0	0.0	0.040715211432372604	2.8672684107304656E-4
31	2.8672684107304656E-4	0.0	0.0	0.044155933525249165	2.8672684107304656E-4
32	2.8672684107304656E-4	0.0	0.0	0.05648518769139017	2.8672684107304656E-4
33	2.8672684107304656E-4	0.0	0.0	0.07798970077186866	2.8672684107304656E-4
34	2.8672684107304656E-4	0.0	0.0	0.10207475542200456	2.8672684107304656E-4
35	2.8672684107304656E-4	0.0	0.0	0.15311213313300684	2.8672684107304656E-4
36	2.8672684107304656E-4	0.0	0.0	0.18866626142606463	2.8672684107304656E-4
37	2.8672684107304656E-4	0.0	0.0	0.2981959147159684	2.8672684107304656E-4
38	2.8672684107304656E-4	0.0	0.0	0.4123131974630409	2.8672684107304656E-4
39	2.8672684107304656E-4	0.0	0.0	0.5539562569531259	2.8672684107304656E-4
40	2.8672684107304656E-4	0.0	0.0	0.7300065373719765	2.8672684107304656E-4
41	2.8672684107304656E-4	0.0	0.0	0.9447649413356883	2.8672684107304656E-4
42	2.8672684107304656E-4	0.0	0.0	1.195650927274604	2.8672684107304656E-4
43	2.8672684107304656E-4	0.0	0.0	1.5024486472227638	2.8672684107304656E-4
44	2.8672684107304656E-4	0.0	0.0	1.879781170074893	2.8672684107304656E-4
45	2.8672684107304656E-4	0.0	0.0	2.1455769517496073	2.8672684107304656E-4
46	2.8672684107304656E-4	0.0	0.0	2.421121446020805	2.8672684107304656E-4
47	2.8672684107304656E-4	0.0	0.0	2.729926253856476	2.8672684107304656E-4
48	2.8672684107304656E-4	0.0	0.0	2.9412439357273112	2.8672684107304656E-4
49	2.8672684107304656E-4	0.0	0.0	3.110986225642555	2.8672684107304656E-4
50	2.8672684107304656E-4	0.0	0.0	3.3850970857083875	2.8672684107304656E-4
51	2.8672684107304656E-4	0.0	0.0	3.684439907788648	2.8672684107304656E-4
52	2.8672684107304656E-4	0.0	0.0	3.9898039935314427	2.8672684107304656E-4
53	2.8672684107304656E-4	0.0	0.0	4.458889105526946	2.8672684107304656E-4
54	2.8672684107304656E-4	0.0	0.0	4.936002569072496	2.8672684107304656E-4
55	2.8672684107304656E-4	0.0	0.0	5.269752612081522	2.8672684107304656E-4
56	2.8672684107304656E-4	0.0	0.0	5.5960477572226495	2.8672684107304656E-4
57	2.8672684107304656E-4	0.0	0.0	5.876179880951016	2.8672684107304656E-4
58	2.8672684107304656E-4	0.0	0.0	6.103267539080869	2.8672684107304656E-4
59	2.8672684107304656E-4	0.0	0.0	6.333509192462524	2.8672684107304656E-4
60	2.8672684107304656E-4	0.0	0.0	6.484900964549094	2.8672684107304656E-4
61	2.8672684107304656E-4	0.0	0.0	6.667545962312624	2.8672684107304656E-4
62	2.8672684107304656E-4	0.0	0.0	6.806321753391979	2.8672684107304656E-4
63	2.8672684107304656E-4	0.0	0.0	7.015345620534229	2.8672684107304656E-4
64	2.8672684107304656E-4	0.0	0.0	7.2252296681997	2.8672684107304656E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	35	0.0012546551	39.999424	52
TCGGTGG	70	1.9219442E-8	39.999424	45
CTCGGTG	115	1.8189894E-12	36.521217	44
CTCTCGG	60	1.2142415E-5	34.9995	42
GTCGATC	40	0.002417301	34.999496	38
CGTTGGT	50	1.70819E-4	34.999496	46
CCGCGAA	40	0.002417301	34.999496	70
TCTCGGT	115	4.0017767E-11	33.477783	43
AGCCGAG	95	7.612471E-9	33.15742	12
TGGTCGC	65	2.1000515E-5	32.30723	49
CCTACGA	55	2.9894628E-4	31.817724	21
CTGCGAG	50	0.007204096	27.9996	60
TTCGGTG	50	0.007204096	27.9996	44
TCGCTTA	90	6.1600986E-6	27.221832	48
CGTATCA	65	7.948827E-4	26.922691	56
CGTCAGA	80	8.6545326E-5	26.249624	1
GTGGTCG	110	1.0136337E-6	25.45418	48
GGTCGCC	70	0.0012249443	24.99964	50
CGGTGTT	70	0.0012249443	24.99964	46
TCGGTGT	100	1.39528765E-5	24.49965	45
>>END_MODULE