FastQCFastQC Report
Wed 25 May 2016
SRR1779689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348764
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT39251.1254028512117078No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG5690.16314757257056348No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT5180.1485245036758381RNA PCR Primer, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAC200.006947998452.58
TCTCGTA7500.043.8666738
TATGCCG7900.043.41772543
GCCGTCT7900.042.97468646
CCGTCTT7950.042.70440347
CGTATGC8050.042.60869641
TCGTATG8150.042.5153440
ATGCCGT8000.042.437544
TGCCGTC8050.042.17391245
ATCTCGT7850.041.91082837
CTCGTAT8200.041.8292739
GTATGCC8450.041.4201242
CGTCTTC8400.041.2548
AATCTCG7750.038.8387136
CTGCTTG9700.035.7216554
GTTAATC7950.035.22012733
GTCTTCT9850.035.17766649
ACACGTA400.002417131335.044
TGCTTGA9850.034.82233455
GCTTGAA10150.034.1379356