##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779688_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33808
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81717345007099	34.0	32.0	34.0	21.0	34.0
2	28.911411500236632	34.0	24.0	34.0	12.0	34.0
3	28.001094415522953	34.0	23.0	34.0	11.0	34.0
4	30.74435044959773	34.0	32.0	34.0	23.0	34.0
5	30.140440132513014	34.0	31.0	34.0	11.0	34.0
6	32.82081164221486	37.0	33.0	38.0	12.0	38.0
7	33.86742782773308	37.0	34.0	38.0	21.0	38.0
8	33.156530998580216	37.0	34.0	38.0	12.0	38.0
9	34.560636535731184	38.0	36.0	38.0	26.0	38.0
10-11	34.39762778040701	38.0	36.0	38.0	23.5	38.0
12-13	33.15673805016564	37.5	32.0	38.0	18.5	38.0
14-15	33.42309512541411	37.0	34.0	38.0	21.0	38.0
16-17	33.02095657832466	37.5	31.5	38.0	18.5	38.0
18-19	33.57141800757218	37.0	34.0	38.0	22.0	38.0
20-21	32.57397657359205	37.0	30.5	38.0	17.0	38.0
22-23	32.5350212967345	37.5	30.5	38.0	17.5	38.0
24-25	34.047917652626595	37.5	35.0	38.0	23.5	38.0
26-27	32.30433625177473	37.0	30.5	38.0	16.0	38.0
28-29	34.65480063890203	38.0	36.0	38.0	25.5	38.0
30-31	35.03840806909607	38.0	37.0	38.0	26.5	38.0
32-33	35.13808861807856	38.0	37.0	38.0	27.0	38.0
34-35	35.19354886417416	38.0	37.0	38.0	27.0	38.0
36-37	32.94270586843351	37.5	30.5	38.0	17.0	38.0
38-39	30.930889138665403	36.5	26.0	38.0	11.0	38.0
40-41	32.45611985328916	37.0	30.5	38.0	16.0	38.0
42-43	33.55080158542357	37.5	33.5	38.0	18.5	38.0
44-45	35.14240712257454	38.0	37.0	38.0	27.0	38.0
46-47	33.60209122101278	37.5	32.0	38.0	19.0	38.0
48-49	32.9778011121628	37.0	30.5	38.0	17.5	38.0
50-51	30.715451964032184	36.5	25.0	38.0	11.0	38.0
52-53	34.36807856128727	37.5	35.0	38.0	24.5	38.0
54-55	33.41252070515854	37.5	33.5	38.0	18.0	38.0
56-57	33.78329093705632	38.0	34.0	38.0	18.5	38.0
58-59	34.95870799810696	38.0	36.5	38.0	26.5	38.0
60-61	35.30985269758637	38.0	37.0	38.0	27.0	38.0
62-63	35.24963026502603	38.0	37.0	38.0	27.0	38.0
64-65	34.89675520586843	38.0	36.5	38.0	25.0	38.0
66-67	32.97675106483672	37.5	33.0	38.0	11.0	38.0
68-69	32.92256270705158	37.0	32.5	38.0	16.0	38.0
70-71	32.368906767628964	37.0	30.5	38.0	11.0	38.0
72-73	31.323355418835778	36.5	26.0	38.0	11.0	38.0
74-75	33.53894048745859	37.5	33.5	38.0	18.0	38.0
76	34.23689659252248	37.0	35.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	9.0
13	46.0
14	119.0
15	183.0
16	266.0
17	404.0
18	452.0
19	462.0
20	399.0
21	334.0
22	251.0
23	219.0
24	225.0
25	255.0
26	283.0
27	343.0
28	449.0
29	560.0
30	736.0
31	1168.0
32	1651.0
33	2596.0
34	4033.0
35	6294.0
36	7767.0
37	4303.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.02579271178419	18.72633696166588	11.60080454330336	28.647065783246568
2	22.006625650733554	18.321107430194036	36.34051112162802	23.33175579744439
3	21.24053478466635	18.196876478939895	34.089564600094654	26.4730241362991
4	14.611926171320397	18.208707998106956	32.96852815901562	34.21083767155702
5	14.668125887363937	29.59358731661145	36.94096071935636	18.797326076668245
6	28.289162328442973	31.504377662091816	17.803478466635116	22.4029815428301
7	27.298272598201613	28.167889256980594	20.663748225272123	23.87008991954567
8	23.169072408897303	31.711429247515383	18.903809749171792	26.21568859441552
9	23.722195929957408	17.759110269758636	20.678537624230948	37.840156176053
10-11	24.8210482725982	23.99875769048746	26.64014434453384	24.540049692380503
12-13	24.79738523426408	20.993551822053952	27.949006152389966	26.260056791292
14-15	23.101041173686703	22.85257927117842	24.14960955986749	29.89676999526739
16-17	23.544723142451492	24.597728348319926	26.489292475153807	25.368256034074776
18-19	22.334950307619497	24.664280643634644	27.274609559867486	25.72615948887837
20-21	23.39239233317558	24.58885470894463	27.51715570279224	24.501597255087553
22-23	23.135056791292	23.782832465688593	27.75970184571699	25.322408897302413
24-25	22.712079981069568	25.458471367723618	25.07098911500237	26.758459536204448
26-27	21.965215333648842	24.125946521533365	28.623402744912447	25.28543539990535
28-29	22.985683861807857	25.563476100331282	26.047089446284904	25.403750591575957
30-31	23.041883577851397	25.439245149077138	26.24822527212494	25.270646000946524
32-33	22.66327496450544	25.418539990534782	26.338440605773783	25.57974443918599
34-35	22.505028395646	24.96154756270705	26.520350212967347	26.0130738286796
36-37	22.715037860861333	23.739943208708	29.821344060577378	23.72367486985329
38-39	23.114351632749646	22.945752484619025	31.80608140085187	22.13381448177946
40-41	23.012304779933743	23.70296971131093	25.516150023663037	27.768575485092285
42-43	21.861689540937057	24.71456460009465	25.93025319451018	27.49349266445812
44-45	21.911973497397067	25.102046852815903	27.286441079034546	25.699538570752484
46-47	22.435518220539517	24.476455276857546	28.558329389493608	24.52969711310932
48-49	22.630738286796024	24.30933506862281	28.474029815428302	24.585896829152865
50-51	22.655880265026028	22.985683861807857	31.5058566019877	22.85257927117842
52-53	21.79217936583057	25.306140558447705	27.12375769048746	25.777922385234262
54-55	22.428123521060105	24.486807856128724	28.1590156176053	24.926053005205866
56-57	22.505028395646	24.59920728821581	27.64138665404638	25.254377662091816
58-59	21.3810340747752	24.924574065309983	27.65913393279697	26.035257927117843
60-61	21.66646947468055	25.183388547089447	27.197704685281593	25.952437292948417
62-63	21.586606720302886	25.30318267865594	26.758459536204448	26.351751064836726
64-65	21.36328679602461	25.369734973970658	26.924100804543304	26.34287742546143
66-67	20.591280170373878	24.219119734973972	24.94971604353999	30.239884051112163
68-69	20.608507868891255	24.79440302922731	25.10945450242575	29.487634599455685
70-71	20.842699952673925	23.96474207288216	24.355182205395174	30.837375769048748
72-73	20.820515854235683	23.275556081400854	23.621628017037388	32.28230004732608
74-75	21.885352579271178	25.496923805016564	25.44811878845244	27.169604827259818
76	22.453265499290108	25.43480832938949	25.008873639375295	27.103052531945103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.5
14	2.5
15	3.5
16	8.5
17	13.0
18	16.5
19	21.5
20	29.5
21	36.0
22	41.5
23	70.0
24	132.0
25	171.0
26	186.5
27	232.0
28	290.0
29	318.0
30	375.0
31	488.0
32	600.5
33	657.0
34	715.5
35	841.0
36	944.5
37	981.0
38	1073.0
39	1240.0
40	1417.0
41	1558.5
42	1598.0
43	1631.0
44	1662.5
45	1679.0
46	1697.0
47	1660.5
48	1611.5
49	1532.0
50	1465.0
51	1377.5
52	1235.0
53	1105.5
54	1031.0
55	986.0
56	885.0
57	783.0
58	737.0
59	699.0
60	641.0
61	584.5
62	548.0
63	559.5
64	553.5
65	529.0
66	513.5
67	505.0
68	491.5
69	462.5
70	435.5
71	424.0
72	415.0
73	380.5
74	303.5
75	252.0
76	226.0
77	185.0
78	142.5
79	115.0
80	91.0
81	59.5
82	41.0
83	30.0
84	25.0
85	15.5
86	6.5
87	2.0
88	1.5
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.011831519167061051
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	33808.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.07146237576904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.25974182651707	96.36476573592049
2	1.5442152249969838	3.028868906767629
3	0.16588249487272289	0.48805016564126835
4	0.030160453613222345	0.1183151916706105
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.002957879791765263	0.0
20	0.0	0.0	0.0	0.002957879791765263	0.0
21	0.0	0.0	0.0	0.002957879791765263	0.0
22	0.0	0.0	0.0	0.023663038334122102	0.0
23	0.0	0.0	0.0	0.026620918125887363	0.0
24	0.0	0.0	0.0	0.029578797917652628	0.0
25	0.0	0.0	0.0	0.029578797917652628	0.0
26	0.0	0.0	0.0	0.029578797917652628	0.0
27	0.0	0.0	0.0	0.035494557501183154	0.0
28	0.0	0.0	0.0	0.041410317084713676	0.0
29	0.0	0.0	0.0	0.04436819687647894	0.0
30	0.0	0.0	0.0	0.047326076668244205	0.0
31	0.0	0.0	0.0	0.050283956460009466	0.0
32	0.0	0.0	0.0	0.06211547562707052	0.0
33	0.0	0.0	0.0	0.07986275437766209	0.0
34	0.0	0.0	0.0	0.09465215333648841	0.0
35	0.0	0.0	0.0	0.12423095125414103	0.0
36	0.0	0.0	0.0	0.14789398958826314	0.0
37	0.0	0.0	0.0	0.23958826313298628	0.0
38	0.0	0.0	0.0	0.3164931377188831	0.0
39	0.0	0.0	0.0	0.4288925698059631	0.0
40	0.0	0.0	0.0	0.5649550402271651	0.0
41	0.0	0.0	0.0	0.7394699479413157	0.0
42	0.0	0.0	0.0	0.8755324183625177	0.0
43	0.0	0.0	0.0	1.0530052058684336	0.0
44	0.0	0.0	0.0	1.3073828679602462	0.0
45	0.0	0.0	0.0	1.4434453383814483	0.0
46	0.0	0.0	0.0	1.609086606720303	0.0
47	0.0	0.0	0.0	1.8338854708944627	0.0
48	0.0	0.0	0.0	1.9965688594415523	0.0
49	0.0	0.0	0.0	2.150378608613346	0.0
50	0.0	0.0	0.0	2.387008991954567	0.0
51	0.0	0.0	0.0	2.6650496923805016	0.0
52	0.0	0.0	0.0	2.9342167534311407	0.0
53	0.0	0.0	0.0	3.232962612399432	0.0
54	0.0	0.0	0.0	3.5287505915759585	0.0
55	0.0	0.0	0.0	3.703265499290109	0.0
56	0.0	0.0	0.0	3.951727401798391	0.0
57	0.0	0.0	0.0	4.155821107430194	0.0
58	0.0	0.0	0.0	4.318504495977283	0.0
59	0.0	0.0	0.0	4.493019403691434	0.0
60	0.0	0.0	0.0	4.611334595362044	0.0
61	0.0	0.0	0.0	4.7385234264079505	0.0
62	0.0	0.0	0.0	4.85092285849503	0.0
63	0.0	0.0	0.0	5.01360624704212	0.0
64	0.0	0.0	0.0	5.1822053951727405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGTAT	15	0.0022062175	70.0	1
TAAAAGG	15	0.0022062175	70.0	14
GTGGTCG	25	2.3578743E-4	55.999996	48
AGAGTAA	20	0.006894094	52.5	50
GGTGGTC	40	4.500393E-5	43.75	47
TGGTGGT	40	0.0023864757	35.0	46
>>END_MODULE