FastQCFastQC Report
Wed 25 May 2016
SRR1779688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33808
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT9432.7892806436346427No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT3000.8873639375295789Illumina PCR Primer Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1380.4081874112636063No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1130.33424041646947467No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1000.2957879791765263No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC920.2721249408424042No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT530.15676762896355892No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC450.13310459062943683No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC370.10944155229531471No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC360.10648367250354945No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG350.10352579271178418No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT150.002206217570.04
TTTATAC252.3578743E-455.9999963
AAGAGAT200.00689409452.56
ATCAAAA200.00689409452.555
CACACTA200.00689409452.535
AAAGAAG200.00689409452.557
CTATACA503.3813612E-642.01
ATCTCGT2550.032.94117737
TCTCGTA2550.032.94117738
CGTATGC2700.032.40740641
CTCGTAT2700.032.40740639
AGATCTC2400.032.08333235
TGCTTGA2750.031.81818255
TATGCCG2750.031.81818243
GTATGCC2750.031.81818242
TCGTATG2750.031.81818240
AAGATCT2450.031.4285734
CTGCTTG2800.031.2554
CGTCTTC2800.031.2548
GCTTGAA2800.031.2556