##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779688_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33808
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.112665641268336	34.0	34.0	34.0	31.0	34.0
2	33.308536441079035	34.0	34.0	34.0	33.0	34.0
3	33.38201017510649	34.0	34.0	34.0	34.0	34.0
4	33.43897893989588	34.0	34.0	34.0	34.0	34.0
5	33.40224207288216	34.0	34.0	34.0	34.0	34.0
6	36.6370681495504	38.0	37.0	38.0	34.0	38.0
7	36.74186583057264	38.0	37.0	38.0	34.0	38.0
8	36.36905466161855	38.0	37.0	38.0	34.0	38.0
9	36.4529105537151	38.0	37.0	38.0	34.0	38.0
10-11	36.383001064836726	38.0	37.0	38.0	34.0	38.0
12-13	36.37955513487932	38.0	37.0	38.0	34.0	38.0
14-15	36.45362044486512	38.0	37.0	38.0	34.0	38.0
16-17	36.3207968528159	38.0	37.0	38.0	34.0	38.0
18-19	36.38621036441079	38.0	37.0	38.0	34.0	38.0
20-21	36.43602106010412	38.0	37.0	38.0	34.0	38.0
22-23	36.24488286796024	38.0	37.0	38.0	34.0	38.0
24-25	36.284104353999055	38.0	37.0	38.0	34.0	38.0
26-27	36.086754614292474	38.0	37.0	38.0	33.0	38.0
28-29	35.9929602460956	38.0	36.5	38.0	32.5	38.0
30-31	35.92223734027449	38.0	36.5	38.0	32.0	38.0
32-33	35.907300047326075	38.0	37.0	38.0	32.0	38.0
34-35	35.860210601041175	38.0	36.5	38.0	31.5	38.0
36-37	35.89286559394226	38.0	36.5	38.0	32.0	38.0
38-39	35.7868551822054	38.0	36.0	38.0	31.5	38.0
40-41	35.937677472787506	38.0	36.5	38.0	32.5	38.0
42-43	35.993137718883105	38.0	37.0	38.0	32.0	38.0
44-45	35.978644107903456	38.0	37.0	38.0	32.0	38.0
46-47	36.028706223379075	38.0	37.0	38.0	32.5	38.0
48-49	36.02724207288216	38.0	37.0	38.0	32.5	38.0
50-51	35.984663393279696	38.0	37.0	38.0	32.5	38.0
52-53	35.964120918125886	38.0	37.0	38.0	32.0	38.0
54-55	35.94464327969712	38.0	37.0	38.0	32.0	38.0
56-57	35.93149550402272	38.0	37.0	38.0	32.0	38.0
58-59	35.903277330809274	38.0	37.0	38.0	32.0	38.0
60-61	35.92333175579745	38.0	37.0	38.0	32.0	38.0
62-63	35.849029815428295	38.0	36.0	38.0	32.0	38.0
64-65	35.829182442025555	38.0	36.0	38.0	31.5	38.0
66-67	35.79989943208708	38.0	36.0	38.0	31.0	38.0
68-69	35.612429010884995	38.0	36.0	38.0	31.0	38.0
70-71	35.23581696639849	38.0	36.0	38.0	29.0	38.0
72-73	34.822601159488876	38.0	36.0	38.0	24.5	38.0
74-75	34.79300757217227	38.0	36.0	38.0	25.0	38.0
76	33.87857903454804	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	6.0
24	16.0
25	32.0
26	83.0
27	110.0
28	219.0
29	330.0
30	578.0
31	857.0
32	1409.0
33	2018.0
34	3306.0
35	4880.0
36	7204.0
37	12757.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.87789872219593	19.705395172740182	11.766445811642216	27.650260293421674
2	21.894226218646473	20.95953620444865	33.96533364884051	23.180903928064364
3	20.589801230477995	22.781590156176055	31.23225272124941	25.396355892096544
4	15.528868906767629	19.690605773781353	34.63381448177946	30.14671083767156
5	15.02307146237577	34.58353052531945	33.96533364884051	16.428064363464266
6	30.21769995267392	36.33459536204449	17.655584477046855	15.792120208234737
7	28.200425934690017	29.868670137245623	19.91836251774728	22.012541410317084
8	24.33743492664458	35.92936583057265	19.84145764316138	19.89174159962139
9	24.065309985802177	17.930667297681023	19.421438712730716	38.58258400378609
10-11	25.569391859914813	25.078383814481782	26.80578561287269	22.546438712730716
12-13	25.084299574065312	21.3130028395646	26.839801230477995	26.762896355892096
14-15	24.020941788925697	22.951668244202555	22.175224798864175	30.852165168007573
16-17	25.616717936583054	27.151857548509227	22.540522953147185	24.69090156176053
18-19	25.334240416469473	25.310577378135353	24.075662565073355	25.27951964032182
20-21	23.809453383814482	24.62878608613346	23.772479886417415	27.789280643634644
22-23	24.33151916706105	27.120799810695694	23.143930430667297	25.403750591575957
24-25	26.03969474680549	27.179957406530995	23.44859204921912	23.33175579744439
26-27	25.144936109796497	27.518634642688124	25.010352579271178	22.326076668244202
28-29	23.68965925224799	24.986689540937057	26.87825366777094	24.445397539044013
30-31	25.278040700425937	24.46610269758637	27.425461429247516	22.830395172740182
32-33	23.5950070989115	25.745385707524846	26.631270705158546	24.02833648840511
34-35	23.16315664931377	28.932501183151913	25.52946048272598	22.37488168480833
36-37	25.27508282063417	27.43433506862281	25.036973497397064	22.253608613345953
38-39	23.60831755797444	24.824006152389966	27.05720539517274	24.51047089446285
40-41	23.024136299100807	24.019462849029814	27.64878135352579	25.307619498343588
42-43	25.57974443918599	23.966221012778043	27.58518693800284	22.86884761003313
44-45	23.022657359204924	26.118078561287266	27.610328916232845	23.248935163274965
46-47	25.207051585423567	24.127425461429247	25.915463795551346	24.750059157595835
48-49	24.91717936583057	23.423450070989116	26.08554188357785	25.57382867960246
50-51	22.65144344533838	23.822763842877425	28.407477520113584	25.11831519167061
52-53	22.497633696166588	23.294782300047327	30.77378135352579	23.433802650260294
54-55	22.803774254614293	23.618670137245623	28.755028395645997	24.822527212494084
56-57	24.279756270705157	23.658601514434455	26.875295787979177	25.18634642688121
58-59	22.64552768575485	24.6169545669664	29.70450780880265	23.033009938476102
60-61	23.810932323710364	27.520113582584006	25.845953620444867	22.823000473260766
62-63	21.781826786559392	30.77378135352579	24.557796971131093	22.88659488878372
64-65	21.432796971131093	30.883222905821107	24.684985802177	22.9989943208708
66-67	22.022893989588262	30.99858021769995	24.031294368196875	22.947231424514907
68-69	21.41061287269285	30.989706578324654	24.310814008518694	23.288866540463797
70-71	21.608790818741127	30.30199952673923	24.5740653099858	23.51514434453384
72-73	21.875	28.51987695220066	24.920137245622335	24.684985802177
74-75	21.586606720302886	28.061405584477043	25.717285849503075	24.634701845716993
76	21.811405584477047	28.256625650733554	24.902389966871745	25.02957879791765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	2.5
15	3.5
16	6.5
17	10.0
18	19.5
19	31.0
20	61.0
21	89.0
22	100.0
23	115.0
24	144.5
25	170.0
26	215.5
27	282.0
28	339.5
29	376.0
30	439.0
31	527.0
32	672.5
33	793.0
34	822.5
35	870.5
36	971.0
37	1053.0
38	1122.0
39	1548.5
40	1978.0
41	2025.5
42	2001.0
43	1893.5
44	1732.0
45	1565.5
46	1453.0
47	1468.5
48	1374.0
49	1212.0
50	1160.0
51	1137.5
52	1020.5
53	876.5
54	827.0
55	795.0
56	725.0
57	662.0
58	637.0
59	581.0
60	527.5
61	509.0
62	488.0
63	477.5
64	464.0
65	430.0
66	413.5
67	428.0
68	454.5
69	439.5
70	411.5
71	425.0
72	419.5
73	399.5
74	370.0
75	355.0
76	301.5
77	231.5
78	181.0
79	147.0
80	129.5
81	106.0
82	80.0
83	60.0
84	54.5
85	36.0
86	17.0
87	11.0
88	8.5
89	3.5
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	33808.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.92108376715571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11556908704175	54.69119734973971
2	16.48246235976144	23.379081874112636
3	4.3875380573049165	9.335068622811168
4	1.2386870751136507	3.513961192617132
5	0.3419944113108396	1.2127307146237576
6	0.16682654210284856	0.709891150023663
7	0.09592526170913793	0.4762186464742073
8	0.029194644867998498	0.1656412683388547
9	0.03753597197314092	0.23958826313298628
>10	0.07507194394628185	1.1328679602460956
>50	0.012511990657713641	0.7246805489824893
>100	0.012511990657713641	1.6297917652626597
>500	0.004170663552571213	2.7892806436346427
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	943	2.7892806436346427	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	300	0.8873639375295789	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	138	0.4081874112636063	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	113	0.33424041646947467	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	100	0.2957879791765263	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	92	0.2721249408424042	No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT	53	0.15676762896355892	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	45	0.13310459062943683	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	37	0.10944155229531471	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	36	0.10648367250354945	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	35	0.10352579271178418	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.002957879791765263
16	0.0	0.0	0.0	0.0	0.002957879791765263
17	0.0	0.0	0.0	0.0	0.002957879791765263
18	0.0	0.0	0.0	0.0	0.002957879791765263
19	0.0	0.0	0.0	0.017747278750591577	0.002957879791765263
20	0.0	0.0	0.0	0.029578797917652628	0.002957879791765263
21	0.0	0.0	0.0	0.029578797917652628	0.002957879791765263
22	0.0	0.0	0.0	0.03253667770941789	0.002957879791765263
23	0.0	0.0	0.0	0.041410317084713676	0.002957879791765263
24	0.0	0.0	0.0	0.04436819687647894	0.002957879791765263
25	0.0	0.0	0.0	0.04436819687647894	0.002957879791765263
26	0.0	0.0	0.0	0.04436819687647894	0.002957879791765263
27	0.0	0.0	0.0	0.050283956460009466	0.002957879791765263
28	0.0	0.0	0.0	0.059157595835305256	0.002957879791765263
29	0.0	0.0	0.0	0.06507335541883578	0.002957879791765263
30	0.0	0.0	0.0	0.06803123521060105	0.002957879791765263
31	0.0	0.0	0.0	0.08577851396119261	0.002957879791765263
32	0.0	0.0	0.0	0.10056791292001893	0.002957879791765263
33	0.0	0.0	0.0	0.12718883104590628	0.002957879791765263
34	0.0	0.0	0.0	0.15972550875532418	0.002957879791765263
35	0.0	0.0	0.0	0.20705158542356839	0.002957879791765263
36	0.0	0.0	0.0	0.2869143398012305	0.002957879791765263
37	0.0	0.0	0.0	0.41706105063890203	0.002957879791765263
38	0.0	0.0	0.0	0.5205868433506863	0.002957879791765263
39	0.0	0.0	0.0	0.6536914339801231	0.002957879791765263
40	0.0	0.0	0.0	0.8341221012778041	0.002957879791765263
41	0.0	0.0	0.0	1.0382158069096072	0.002957879791765263
42	0.0	0.0	0.0	1.2245622337908186	0.002957879791765263
43	0.0	0.0	0.0	1.440487458589683	0.002957879791765263
44	0.0	0.0	0.0	1.7066966398485566	0.002957879791765263
45	0.0	0.0	0.0	1.9729058211074302	0.002957879791765263
46	0.0	0.0	0.0	2.230241362991008	0.002957879791765263
47	0.0	0.0	0.0	2.5082820634169427	0.002957879791765263
48	0.0	0.0	0.0	2.7951964032181733	0.002957879791765263
49	0.0	0.0	0.0	3.052531945101751	0.002957879791765263
50	0.0	0.0	0.0	3.32169900615239	0.002957879791765263
51	0.0	0.0	0.0	3.6204448651206813	0.002957879791765263
52	0.0	0.0	0.0	3.963558920965452	0.002957879791765263
53	0.0	0.0	0.0	4.280052058684335	0.002957879791765263
54	0.0	0.0	0.0	4.599503076194983	0.002957879791765263
55	0.0	0.0	0.0	4.865712257453857	0.002957879791765263
56	0.0	0.0	0.0	5.194036914339801	0.002957879791765263
57	0.0	0.0	0.0	5.5016564126833885	0.002957879791765263
58	0.0	0.0	0.0	5.761949834358732	0.002957879791765263
59	0.0	0.0	0.0	5.998580217699953	0.002957879791765263
60	0.0	0.0	0.0	6.2707051585423565	0.002957879791765263
61	0.0	0.0	0.0	6.554661618551822	0.002957879791765263
62	0.0	0.0	0.0	6.841575958353053	0.002957879791765263
63	0.0	0.0	0.0	7.166942735447232	0.002957879791765263
64	0.0	0.0	0.0	7.533719829626124	0.002957879791765263
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGGT	15	0.0022062175	70.0	4
TTTATAC	25	2.3578743E-4	55.999996	3
AAGAGAT	20	0.006894094	52.5	6
ATCAAAA	20	0.006894094	52.5	55
CACACTA	20	0.006894094	52.5	35
AAAGAAG	20	0.006894094	52.5	57
CTATACA	50	3.3813612E-6	42.0	1
ATCTCGT	255	0.0	32.941177	37
TCTCGTA	255	0.0	32.941177	38
CGTATGC	270	0.0	32.407406	41
CTCGTAT	270	0.0	32.407406	39
AGATCTC	240	0.0	32.083332	35
TGCTTGA	275	0.0	31.818182	55
TATGCCG	275	0.0	31.818182	43
GTATGCC	275	0.0	31.818182	42
TCGTATG	275	0.0	31.818182	40
AAGATCT	245	0.0	31.42857	34
CTGCTTG	280	0.0	31.25	54
CGTCTTC	280	0.0	31.25	48
GCTTGAA	280	0.0	31.25	56
>>END_MODULE