FastQCFastQC Report
Wed 25 May 2016
SRR1779687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18492
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCTG150.00219027470.0000142
TTCCGAC150.00219027470.0000119
GTATCAT150.00219027470.0000157
CTTACTC150.00219027470.0000112
CGTATCA150.00219027470.0000156
TTTAGAT150.00219027470.0000137
GCCGTAT150.00219027470.0000154
TCCGACG150.00219027470.0000120
GCCCTAT207.7240074E-570.054
GTGTTCG207.7240074E-570.048
ATCTTAC207.7240074E-570.010
CGCCCTA207.7240074E-570.053
CATCTTA207.7240074E-570.09
GATCTCG409.920768E-961.2541
GTAGATC308.059276E-658.33333638
TGTTCGC308.059276E-658.33333649
TGTAGAT308.059276E-658.33333637
ATACCCA308.059276E-658.3333364
GTTCGCC308.059276E-658.33333650
TGACTCT252.3296979E-456.00000413