##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779687_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18492
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.00654337010599	33.0	23.0	34.0	12.0	34.0
2	26.786988968202465	32.0	21.0	34.0	12.0	34.0
3	26.23193813540991	32.0	21.0	34.0	11.0	34.0
4	27.900605667315595	33.0	23.0	34.0	12.0	34.0
5	27.565704088254382	33.0	23.0	34.0	11.0	34.0
6	29.83609128271685	36.0	23.0	38.0	11.0	38.0
7	30.907906121566082	37.0	26.0	38.0	12.0	38.0
8	30.168829764222366	36.0	24.0	38.0	11.0	38.0
9	31.48896820246593	37.0	27.0	38.0	12.0	38.0
10-11	31.094202898550726	37.0	26.0	38.0	11.0	38.0
12-13	30.41547696301103	35.5	25.0	38.0	11.0	38.0
14-15	30.20035691109669	36.0	24.5	38.0	11.0	38.0
16-17	29.990590525632705	35.5	24.0	38.0	11.0	38.0
18-19	30.52433484750162	36.0	25.5	38.0	11.0	38.0
20-21	29.623729180186025	35.0	23.5	38.0	11.0	38.0
22-23	29.542775254163963	35.5	23.5	38.0	11.0	38.0
24-25	30.57205818732425	36.5	24.5	38.0	11.0	38.0
26-27	29.171641791044777	35.0	22.0	38.0	11.0	38.0
28-29	30.944705818732423	37.0	26.0	38.0	11.0	38.0
30-31	31.40893359290504	37.5	26.5	38.0	11.0	38.0
32-33	31.571679645252	38.0	27.0	38.0	11.0	38.0
34-35	31.596501189703655	38.0	27.0	38.0	11.0	38.0
36-37	29.832873675102746	35.0	24.0	38.0	11.0	38.0
38-39	28.470635950681377	34.0	21.5	37.5	11.0	38.0
40-41	29.25254163962795	34.5	22.0	37.5	11.0	38.0
42-43	30.145441271901362	35.5	24.0	38.0	11.0	38.0
44-45	31.557619511139954	37.5	27.0	38.0	11.0	38.0
46-47	30.34168829764222	36.0	24.5	38.0	11.0	38.0
48-49	29.57860155743024	35.5	23.0	38.0	11.0	38.0
50-51	27.843121349772872	33.0	21.0	37.5	11.0	38.0
52-53	30.60634328358209	36.5	24.5	38.0	11.0	38.0
54-55	29.845554834523035	35.5	23.5	38.0	11.0	38.0
56-57	30.243916288124595	36.0	24.0	38.0	11.0	38.0
58-59	31.162935323383085	37.0	26.0	38.0	11.0	38.0
60-61	31.589390006489293	38.0	27.0	38.0	11.0	38.0
62-63	31.576600692191217	38.0	26.5	38.0	11.0	38.0
64-65	31.308457711442784	37.5	26.0	38.0	11.0	38.0
66-67	29.746728314947006	36.0	23.0	38.0	11.0	38.0
68-69	29.656256759679863	35.5	23.0	38.0	11.0	38.0
70-71	29.191731559593336	34.5	22.0	38.0	11.0	38.0
72-73	28.39425156824573	34.0	21.0	37.5	11.0	38.0
74-75	29.721609344581438	35.5	23.0	38.0	11.0	38.0
76	30.095446679645253	36.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	9.0
13	70.0
14	160.0
15	338.0
16	511.0
17	680.0
18	801.0
19	766.0
20	622.0
21	447.0
22	349.0
23	266.0
24	239.0
25	219.0
26	213.0
27	232.0
28	232.0
29	336.0
30	446.0
31	566.0
32	890.0
33	1255.0
34	1877.0
35	2545.0
36	2958.0
37	1464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.55505083279256	17.63465282284231	12.978585334198572	35.83171101016656
2	18.91628812459442	14.682024659312134	41.26108587497296	25.140601341120483
3	21.08479342418343	14.676616915422885	36.680726800778714	27.557862859614968
4	16.74237508111616	18.797317759030932	27.357776335712742	37.10253082414017
5	16.033960631624485	24.859398658879517	37.10793856802942	21.99870214146658
6	27.56327060350422	32.22474583603721	14.087172831494701	26.124810728963876
7	26.817001946787798	23.783257624918882	17.396712091715337	32.003028336577984
8	21.90677049534934	31.051265412070084	17.391304347826086	29.65065974475449
9	22.2744970798183	14.887518927103613	18.689162881245945	44.14882111183214
10-11	25.17845554834523	21.944624702574085	25.30553752974259	27.57138221933809
12-13	23.44797750378542	17.247999134760978	28.788124594419205	30.515898767034393
14-15	25.20549426779148	20.530499675535367	21.712091715336364	32.5519143413368
16-17	27.46052346960848	19.156932727666018	23.420938784339175	29.96160501838633
18-19	23.44797750378542	19.070408825438026	26.62773091066407	30.853882760112484
20-21	27.255029201817	18.35388276011248	24.832359939433267	29.55872809863725
22-23	28.233830845771145	18.234912394548992	24.113130002163096	29.418126757516767
24-25	24.51600692191218	22.212308025091932	20.784663638330088	32.487021414665804
26-27	25.93553969284015	21.563378758382004	24.624161799697166	27.87691974908068
28-29	25.040558079169372	22.17174994592256	23.023469608479342	29.764222366428726
30-31	24.394332684404066	21.27406446030716	24.40785204412719	29.923750811161582
32-33	24.072571922993728	21.271360588362533	24.651200519143412	30.004866969500327
34-35	24.662016006921913	21.422777417261518	24.213173264114214	29.70203331170236
36-37	26.441163746484968	18.343067272333982	28.961172398875192	26.25459658230586
38-39	26.29245078953061	17.13443651308674	32.989941596365995	23.583171101016656
40-41	25.075708414449494	21.890547263681594	22.885572139303484	30.148172182565435
42-43	21.682349123945492	22.2961280553753	25.010815487778498	31.010707332900715
44-45	22.031148604802077	19.72744970798183	27.790395846852693	30.4510058403634
46-47	25.98961713173264	19.246160501838634	26.698031581224313	28.066190785204416
48-49	25.81116158338741	19.075816569327277	28.095933376595283	27.017088470690027
50-51	29.902119835604584	17.577871512005192	27.820138438243564	24.699870214146657
52-53	21.076681808349555	21.65801427644387	27.13876270819814	30.126541207008433
54-55	25.30283365779797	19.803158122431324	25.186567164179102	29.707441055591605
56-57	25.392061431970582	19.9978369024443	24.759355396928402	29.850746268656714
58-59	21.479558728098638	21.59041747782825	26.262708198139734	30.667315595933374
60-61	21.822950465065976	20.614319705818733	25.297425913908718	32.26530391520658
62-63	21.31462253947653	22.333982262600045	25.202790395846854	31.148604802076573
64-65	21.776984642007356	23.047804455980963	24.43759463551806	30.737616266493617
66-67	20.844148821111833	25.50562405364482	20.665693272766603	32.984533852476744
68-69	21.088067490468593	26.13362896465944	20.495903523240408	32.28240002163156
70-71	20.906337875838197	26.811594202898554	19.581440622972096	32.70062729829115
72-73	20.103287908284663	27.357776335712742	19.240752757949384	33.298182998053214
74-75	22.7125243348475	24.767467012762275	21.771576898118106	30.748431754272115
76	23.956305429374865	22.490806835388277	21.69586848366861	31.857019251568246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	1.0
19	6.0
20	9.5
21	8.0
22	13.5
23	19.0
24	23.0
25	27.0
26	32.5
27	41.5
28	67.5
29	90.0
30	100.5
31	121.5
32	147.5
33	163.0
34	200.5
35	281.0
36	342.0
37	360.0
38	392.5
39	476.0
40	539.0
41	587.0
42	623.0
43	669.5
44	729.5
45	751.5
46	760.0
47	738.5
48	726.5
49	728.5
50	721.0
51	689.0
52	665.0
53	647.5
54	622.0
55	588.0
56	545.5
57	552.5
58	568.0
59	548.5
60	532.5
61	508.0
62	480.0
63	476.5
64	510.0
65	531.5
66	491.5
67	467.0
68	491.5
69	497.5
70	454.0
71	429.0
72	410.5
73	366.0
74	326.0
75	312.0
76	274.0
77	211.0
78	158.0
79	130.0
80	108.0
81	71.0
82	40.0
83	24.0
84	21.0
85	15.5
86	9.0
87	5.0
88	3.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0027038719446247027
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	18492.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.99956738048886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.04954389031518	98.05861994375947
2	0.8903698039001475	1.7629245078953062
3	0.060086305784672535	0.17845554834523036
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0054077438892494055	0.0
10	0.0	0.0	0.0	0.0054077438892494055	0.0
11	0.0	0.0	0.0	0.0054077438892494055	0.0
12	0.0	0.0	0.0	0.0054077438892494055	0.0
13	0.0	0.0	0.0	0.010815487778498811	0.0
14	0.0	0.0	0.0	0.016223231667748216	0.0
15	0.0	0.0	0.0	0.016223231667748216	0.0
16	0.0	0.0	0.0	0.016223231667748216	0.0
17	0.0	0.0	0.0	0.016223231667748216	0.0
18	0.0	0.0	0.0	0.016223231667748216	0.0
19	0.0	0.0	0.0	0.027038719446247025	0.0
20	0.0	0.0	0.0	0.043261951113995244	0.0
21	0.0	0.0	0.0	0.043261951113995244	0.0
22	0.0	0.0	0.0	0.14060134112048453	0.0
23	0.0	0.0	0.0	0.15141682889898334	0.0
24	0.0	0.0	0.0	0.18927103612372917	0.0
25	0.0	0.0	0.0	0.200086523902228	0.0
26	0.0	0.0	0.0	0.200086523902228	0.0
27	0.0	0.0	0.0	0.2163097555699762	0.0
28	0.0	0.0	0.0	0.23794073112697384	0.0
29	0.0	0.0	0.0	0.25416396279472203	0.0
30	0.0	0.0	0.0	0.28120268224096906	0.0
31	0.0	0.0	0.0	0.2920181700194679	0.0
32	0.0	0.0	0.0	0.3136491455764655	0.0
33	0.0	0.0	0.0	0.34068786502271253	0.0
34	0.0	0.0	0.0	0.4218040233614536	0.0
35	0.0	0.0	0.0	0.5083279255894441	0.0
36	0.0	0.0	0.0	0.5569976205926888	0.0
37	0.0	0.0	0.0	0.7841228639411637	0.0
38	0.0	0.0	0.0	0.9301319489508977	0.0
39	0.0	0.0	0.0	1.1734804239671208	0.0
40	0.0	0.0	0.0	1.4222366428725937	0.0
41	0.0	0.0	0.0	1.654769630110318	0.0
42	0.0	0.0	0.0	1.8764871295695436	0.0
43	0.0	0.0	0.0	2.136058836253515	0.0
44	0.0	0.0	0.0	2.4767467012762276	0.0
45	0.0	0.0	0.0	2.6389790179537096	0.0
46	0.0	0.0	0.0	2.9201817001946786	0.0
47	0.0	0.0	0.0	3.190568894657149	0.0
48	0.0	0.0	0.0	3.4068786502271253	0.0
49	0.0	0.0	0.0	3.671858100800346	0.0
50	0.0	0.0	0.0	4.00173047804456	0.0
51	0.0	0.0	0.0	4.261302184728531	0.0
52	0.0	0.0	0.0	4.520873891412503	0.0
53	0.0	0.0	0.0	4.780445598096474	0.0
54	0.0	0.0	0.0	5.126541207008436	0.0
55	0.0	0.0	0.0	5.348258706467662	0.0
56	0.0	0.0	0.0	5.580791693705386	0.0
57	0.0	0.0	0.0	5.851178888167857	0.0
58	0.0	0.0	0.0	6.035042180402336	0.0
59	0.0	0.0	0.0	6.283798399307809	0.0
60	0.0	0.0	0.0	6.402768764871296	0.0
61	0.0	0.0	0.0	6.510923642656284	0.0
62	0.0	0.0	0.0	6.613670776552023	0.0
63	0.0	0.0	0.0	6.775903093229505	0.0
64	0.0	0.0	0.0	6.954358641574735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCTG	15	0.002190274	70.00001	42
TTCCGAC	15	0.002190274	70.00001	19
GTATCAT	15	0.002190274	70.00001	57
CTTACTC	15	0.002190274	70.00001	12
CGTATCA	15	0.002190274	70.00001	56
TTTAGAT	15	0.002190274	70.00001	37
GCCGTAT	15	0.002190274	70.00001	54
TCCGACG	15	0.002190274	70.00001	20
GCCCTAT	20	7.7240074E-5	70.0	54
GTGTTCG	20	7.7240074E-5	70.0	48
ATCTTAC	20	7.7240074E-5	70.0	10
CGCCCTA	20	7.7240074E-5	70.0	53
CATCTTA	20	7.7240074E-5	70.0	9
GATCTCG	40	9.920768E-9	61.25	41
GTAGATC	30	8.059276E-6	58.333336	38
TGTTCGC	30	8.059276E-6	58.333336	49
TGTAGAT	30	8.059276E-6	58.333336	37
ATACCCA	30	8.059276E-6	58.333336	4
GTTCGCC	30	8.059276E-6	58.333336	50
TGACTCT	25	2.3296979E-4	56.000004	13
>>END_MODULE