SRR1779687_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779687_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18492
Sequences flagged as poor quality	0
Sequence length	76
%GC	52

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	1920	10.382868267358859	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	467	2.5254163962794722	RNA PCR Primer, Index 30 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	291	1.5736534717715769	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	199	1.0761410339606317	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	197	1.0653255461821327	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	185	1.00043261951114	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	93	0.5029201817001947	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	76	0.4109885355829548	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	71	0.38394981613670776	RNA PCR Primer, Index 30 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	69	0.3731343283582089	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	58	0.3136491455764655	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	58	0.3136491455764655	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	48	0.25957170668397145	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	40	0.2163097555699762	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	40	0.2163097555699762	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	39	0.21090201168072678	No Hit
TCTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	30	0.16223231667748217	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGTCTT	24	0.12978585334198572	No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA	21	0.11356262167423752	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	21	0.11356262167423752	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACAC	15	0.002190274	70.00001	10
ATAAGCC	15	0.002190274	70.00001	50
TAAGCCT	15	0.002190274	70.00001	51
TCTTTAT	30	9.6952135E-8	70.00001	1
AAGCCTG	15	0.002190274	70.00001	52
ACTCGTA	25	2.3296979E-4	56.000004	38
AGCCTGC	20	0.0068446524	52.5	53
CATGAAA	20	0.0068446524	52.5	29
CTTTATA	40	7.087492E-7	52.5	2
TTTATAC	40	7.087492E-7	52.5	3
GCCGGAA	30	5.729468E-4	46.666668	32
GAAAAAA	430	0.0	40.697674	60
TGAAAAA	430	0.0	40.697674	59
CCGGAAC	35	0.0012239347	40.000004	33
AAACATT	35	0.0012239347	40.000004	34
AACTCGT	35	0.0012239347	40.000004	37
CGGAACT	35	0.0012239347	40.000004	34
TATGCCG	430	0.0	39.88372	43
TCTGCTT	425	0.0	39.52941	53
CTGCTTG	425	0.0	39.52941	54

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content