##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779687_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18492
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13816785637032	34.0	34.0	34.0	31.0	34.0
2	33.344905905256326	34.0	34.0	34.0	33.0	34.0
3	33.39536015574303	34.0	34.0	34.0	34.0	34.0
4	33.47377244213714	34.0	34.0	34.0	34.0	34.0
5	33.45425048669695	34.0	34.0	34.0	34.0	34.0
6	36.70268224096907	38.0	37.0	38.0	34.0	38.0
7	36.76773739995674	38.0	37.0	38.0	34.0	38.0
8	36.53104044992429	38.0	37.0	38.0	34.0	38.0
9	36.504326195111396	38.0	37.0	38.0	34.0	38.0
10-11	36.36477936404932	38.0	37.0	38.0	34.0	38.0
12-13	36.39536015574302	38.0	37.0	38.0	34.0	38.0
14-15	36.44216417910448	38.0	37.0	38.0	34.0	38.0
16-17	36.27057646549859	38.0	36.5	38.0	33.5	38.0
18-19	36.2402930997188	38.0	37.0	38.0	34.0	38.0
20-21	36.438135409906984	38.0	37.0	38.0	34.0	38.0
22-23	36.23426346528228	38.0	37.0	38.0	34.0	38.0
24-25	36.16047479991347	38.0	36.0	38.0	33.0	38.0
26-27	35.96055050832793	38.0	36.0	38.0	32.0	38.0
28-29	35.84963768115942	38.0	36.0	38.0	31.0	38.0
30-31	35.86556348691326	38.0	36.5	38.0	31.5	38.0
32-33	35.544208306294614	38.0	36.0	38.0	31.0	38.0
34-35	35.30405040017305	38.0	36.0	38.0	29.0	38.0
36-37	35.105586199437596	38.0	35.0	38.0	29.0	38.0
38-39	34.09747458360372	37.0	34.0	38.0	23.5	38.0
40-41	35.18305213065109	37.5	35.5	38.0	29.0	38.0
42-43	35.750621890547265	38.0	36.0	38.0	31.0	38.0
44-45	35.73507462686567	38.0	36.0	38.0	31.0	38.0
46-47	35.731694786934895	38.0	36.0	38.0	31.0	38.0
48-49	35.68651308674021	38.0	36.0	38.0	31.0	38.0
50-51	35.485831711010164	38.0	36.0	38.0	31.0	38.0
52-53	35.59966471987887	38.0	36.0	38.0	31.0	38.0
54-55	35.579088254380274	38.0	36.0	38.0	31.0	38.0
56-57	35.4888330088687	38.0	36.0	38.0	31.0	38.0
58-59	35.343094311053434	38.0	36.0	38.0	30.5	38.0
60-61	35.41320571057754	38.0	36.0	38.0	31.0	38.0
62-63	35.498864373783256	38.0	36.0	38.0	31.0	38.0
64-65	35.58595608911962	38.0	36.0	38.0	31.0	38.0
66-67	35.53252757949383	38.0	36.0	38.0	31.0	38.0
68-69	34.989292667099285	38.0	35.5	38.0	27.5	38.0
70-71	33.49004975124379	37.5	32.5	38.0	21.5	38.0
72-73	31.338741077222583	37.0	26.0	38.0	11.0	38.0
74-75	31.003487994808566	37.0	25.0	38.0	11.0	38.0
76	30.25367726584469	36.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	6.0
23	14.0
24	18.0
25	35.0
26	72.0
27	111.0
28	203.0
29	329.0
30	480.0
31	702.0
32	1049.0
33	1466.0
34	2245.0
35	3054.0
36	3294.0
37	5413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.1440622972096	17.126324897252864	12.286394116374648	37.44321868916288
2	17.00735453168938	15.53104044992429	43.19705818732425	24.26454683106208
3	18.256543370105994	19.743672939649578	38.422020333117025	23.577763357127406
4	14.292667099286177	26.492537313432834	29.969716634220205	29.245078953060784
5	14.422452952628165	32.48431754272117	37.82176076141034	15.271468743240321
6	27.763357127406447	39.1196192948302	14.995673804888602	18.121349772874755
7	24.935107073329007	27.833657797966687	16.19078520441272	31.040449924291586
8	20.711659095825222	41.23945489941596	15.698680510491023	22.35020549426779
9	20.230369889682024	16.969500324464633	14.108803807051698	48.69132597880164
10-11	23.612913692407528	25.946355180618646	26.04099069868051	24.399740428293313
12-13	23.45879299156392	15.390439108803807	27.668721609344583	33.48204629028769
14-15	23.899524118537744	16.47469175859831	17.302076573653473	42.32370754921047
16-17	31.481181051265413	25.735453168937923	15.474259139087174	27.309106640709498
18-19	31.316244862643305	20.622431321652606	16.004218040233614	32.057105775470475
20-21	23.710253082414017	18.191650443434998	15.682457278823275	42.41563919532771
22-23	24.348366861345447	26.733181916504435	15.928509625784123	32.989941596365995
24-25	31.99762059268873	27.409149902660612	15.674345662989401	24.91888384166126
26-27	31.20808998485832	29.458684836686132	17.2398875189271	22.093337659528444
28-29	23.98334414882111	27.603828682673587	19.85993943326844	28.552887735236858
30-31	23.29385680294181	18.337659528444732	33.106208089984854	25.262275578628596
32-33	32.25448842742808	16.493618862210685	20.61702357776336	30.63486913259788
34-35	32.154445165476965	18.110534285096257	18.021306510923644	31.713714038503138
36-37	32.58706467661692	24.145576465498593	18.978477179320787	24.288881678563705
38-39	23.512870430456413	19.60036772658447	26.20051914341337	30.68624269954575
40-41	22.277200951762925	17.226368159203982	28.423101881894873	32.07332900713823
42-43	30.72680077871512	16.793748648064028	28.387951546614755	24.0914990266061
44-45	22.772009517629247	24.697166342202035	28.939541423318193	23.591282716850532
46-47	30.131948950897687	17.242591390871727	21.990590525632705	30.63486913259788
48-49	29.61010166558512	15.793316028552887	20.700843608046725	33.89573869781527
50-51	22.317759030932294	16.29353233830846	29.172074410555915	32.21663422020333
52-53	20.568353882760114	16.304347826086957	37.751460090850095	25.375838200302837
54-55	20.635950681375732	16.08803807051698	32.02465931213498	31.25135193597231
56-57	28.601557430240103	16.152930997187973	24.032013843824355	31.21349772874757
58-59	22.761194029850746	19.695003244646333	33.939000648929266	23.60480207657365
60-61	28.201384382435645	29.499242915855504	20.635950681375732	21.66342202033312
62-63	20.146549859398657	41.21512005191434	16.77752541639628	21.86080467229072
64-65	18.948734587929916	43.305213065109236	16.504434349989182	21.241617996971662
66-67	19.413800562405363	42.596798615617566	16.32868267358858	21.66071814838849
68-69	18.98658879515466	42.445381786718585	16.615293099718798	21.95273631840796
70-71	19.546290287691974	39.54142331819165	17.46701276227558	23.445273631840795
72-73	20.252000865239022	33.87140385031365	19.86534717715769	26.011248107289635
74-75	20.40612156608263	31.697490806835386	20.679212632489723	27.21717499459226
76	21.149686350854424	31.500108154877786	19.5598096474151	27.790395846852693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	1.5
11	0.0
12	0.0
13	0.5
14	0.5
15	1.0
16	1.5
17	1.0
18	5.0
19	8.5
20	8.0
21	8.0
22	12.5
23	19.0
24	33.0
25	45.0
26	43.0
27	41.5
28	70.5
29	99.0
30	110.0
31	140.5
32	169.5
33	179.0
34	204.0
35	240.5
36	272.5
37	293.0
38	299.0
39	345.5
40	489.0
41	1057.0
42	1522.0
43	1637.5
44	1670.0
45	1309.0
46	1031.0
47	905.5
48	651.0
49	460.0
50	398.0
51	377.5
52	341.0
53	297.0
54	269.0
55	282.5
56	324.5
57	328.0
58	303.0
59	316.5
60	334.0
61	348.5
62	359.0
63	371.5
64	378.0
65	419.0
66	447.5
67	429.0
68	441.5
69	465.0
70	454.5
71	433.0
72	437.5
73	406.5
74	347.0
75	323.0
76	308.5
77	264.5
78	195.0
79	155.0
80	145.0
81	103.5
82	65.0
83	58.0
84	42.5
85	23.0
86	13.0
87	7.0
88	3.5
89	0.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	18492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.75059485182782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44632366031689	48.26411421155094
2	15.364073348762686	18.667531905688946
3	3.4359978636282715	6.262167423750811
4	0.8278440448638062	2.011680726800779
5	0.23144027060708564	0.7030067056024227
6	0.16912942852056256	0.6164828033744322
7	0.08011393982552964	0.34068786502271253
8	0.03560619547801318	0.17304780445598097
9	0.03560619547801318	0.1946787800129786
>10	0.2670464660850988	2.844473285745187
>50	0.05340929321701976	2.298291152930997
>100	0.04450774434751647	7.240969067704954
>500	0.0	0.0
>1k	0.008901548869503294	10.382868267358859
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	1920	10.382868267358859	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	467	2.5254163962794722	RNA PCR Primer, Index 30 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	291	1.5736534717715769	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	199	1.0761410339606317	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	197	1.0653255461821327	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	185	1.00043261951114	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	93	0.5029201817001947	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	76	0.4109885355829548	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	71	0.38394981613670776	RNA PCR Primer, Index 30 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	69	0.3731343283582089	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	58	0.3136491455764655	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	58	0.3136491455764655	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	48	0.25957170668397145	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	40	0.2163097555699762	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	40	0.2163097555699762	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	39	0.21090201168072678	No Hit
TCTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	30	0.16223231667748217	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGTCTT	24	0.12978585334198572	No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA	21	0.11356262167423752	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	21	0.11356262167423752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0054077438892494055	0.0
10	0.0	0.0	0.0	0.0054077438892494055	0.0
11	0.0	0.0	0.0	0.0054077438892494055	0.0
12	0.0	0.0	0.0	0.0054077438892494055	0.0
13	0.0	0.0	0.0	0.010815487778498811	0.0
14	0.0	0.0	0.0	0.010815487778498811	0.0
15	0.0	0.0	0.0	0.010815487778498811	0.0
16	0.0	0.0	0.0	0.010815487778498811	0.0
17	0.0	0.0	0.0	0.010815487778498811	0.0
18	0.0	0.0	0.0	0.010815487778498811	0.0
19	0.0	0.0	0.0	0.016223231667748216	0.0
20	0.0	0.0	0.0	0.03244646333549643	0.0
21	0.0	0.0	0.0	0.03244646333549643	0.0
22	0.0	0.0	0.0	0.03244646333549643	0.0
23	0.0	0.0	0.0	0.04866969500324465	0.0
24	0.0	0.0	0.0	0.06489292667099286	0.0
25	0.0	0.0	0.0	0.06489292667099286	0.0
26	0.0	0.0	0.0	0.06489292667099286	0.0
27	0.0	0.0	0.0	0.08111615833874107	0.0
28	0.0	0.0	0.0	0.1081548777849881	0.0
29	0.0	0.0	0.0	0.15682457278823275	0.0
30	0.0	0.0	0.0	0.2054942677914774	0.0
31	0.0	0.0	0.0	0.24875621890547264	0.0
32	0.0	0.0	0.0	0.2974259139087173	0.0
33	0.0	0.0	0.0	0.36772658446895956	0.0
34	0.0	0.0	0.0	0.5245511572571923	0.0
35	0.0	0.0	0.0	0.6489292667099286	0.0
36	0.0	0.0	0.0	0.8382003028336578	0.0
37	0.0	0.0	0.0	1.3032662773091066	0.0
38	0.0	0.0	0.0	1.6060999351070733	0.0
39	0.0	0.0	0.0	1.8927103612372917	0.0
40	0.0	0.0	0.0	2.1955440190352586	0.0
41	0.0	0.0	0.0	2.536231884057971	0.0
42	0.0	0.0	0.0	2.8228423101881894	0.0
43	0.0	0.0	0.0	3.163530175210902	0.0
44	0.0	0.0	0.0	3.5853341985723555	0.0
45	0.0	0.0	0.0	3.942245295262816	0.0
46	0.0	0.0	0.0	4.293748648064028	0.0
47	0.0	0.0	0.0	4.693921695868483	0.0
48	0.0	0.0	0.0	5.040017304780446	0.0
49	0.0	0.0	0.0	5.553752974259139	0.0
50	0.0	0.0	0.0	5.948518278174346	0.0
51	0.0	0.0	0.0	6.283798399307809	0.0
52	0.0	0.0	0.0	6.613670776552023	0.0
53	0.0	0.0	0.0	6.894873458792992	0.0
54	0.0	0.0	0.0	7.257192299372702	0.0
55	0.0	0.0	0.0	7.597880164395415	0.0
56	0.0	0.0	0.0	7.884490590525632	0.0
57	0.0	0.0	0.0	8.27384815055159	0.0
58	0.0	0.0	0.0	8.560458576681809	0.0
59	0.0	0.0	0.0	8.90114644170452	0.0
60	0.0	0.0	0.0	9.18234912394549	0.0
61	0.0	0.0	0.0	9.44732857451871	0.0
62	0.0	0.0	0.0	9.72853125675968	0.0
63	0.0	0.0	0.0	10.0151416828899	0.0
64	0.0	0.0	0.0	10.399091499026605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACAC	15	0.002190274	70.00001	10
ATAAGCC	15	0.002190274	70.00001	50
TAAGCCT	15	0.002190274	70.00001	51
TCTTTAT	30	9.6952135E-8	70.00001	1
AAGCCTG	15	0.002190274	70.00001	52
ACTCGTA	25	2.3296979E-4	56.000004	38
AGCCTGC	20	0.0068446524	52.5	53
CATGAAA	20	0.0068446524	52.5	29
CTTTATA	40	7.087492E-7	52.5	2
TTTATAC	40	7.087492E-7	52.5	3
GCCGGAA	30	5.729468E-4	46.666668	32
GAAAAAA	430	0.0	40.697674	60
TGAAAAA	430	0.0	40.697674	59
CCGGAAC	35	0.0012239347	40.000004	33
AAACATT	35	0.0012239347	40.000004	34
AACTCGT	35	0.0012239347	40.000004	37
CGGAACT	35	0.0012239347	40.000004	34
TATGCCG	430	0.0	39.88372	43
TCTGCTT	425	0.0	39.52941	53
CTGCTTG	425	0.0	39.52941	54
>>END_MODULE