FastQCFastQC Report
Wed 25 May 2016
SRR1779686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59404
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA150.002214503270.0000156
ATAGGGT150.002214503270.000016
ATCCCGG150.002214503270.0000142
CATCGGA150.002214503270.000019
ACATCGG150.002214503270.000018
GCTGTAG150.002214503270.0000151
TTCCGAC207.866982E-570.019
GACGCTT207.866982E-570.014
CGGTGGT550.063.6363646
TCTCGGT401.0442818E-861.2543
AGTGTAG308.2830775E-658.33333635
CTTCCGA252.3725611E-455.99999618
CTCGGTG509.549694E-1055.99999644
TGTCGAT252.3725611E-455.99999637
AGTGTCG252.3725611E-455.99999635
CGACGAC452.64572E-854.44444322
TCGTTTT200.006919788752.539
ACGCCGG200.006919788752.529
TATATAG200.006919788752.544
TTCCCGC200.006919788752.552
Produced by FastQC (version 0.11.5)