##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779686_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59404
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.680492896101274	34.0	31.0	34.0	12.0	34.0
2	28.040115143761362	34.0	23.0	34.0	12.0	34.0
3	27.095515453504813	32.0	23.0	34.0	11.0	34.0
4	29.569170426233924	34.0	27.0	34.0	12.0	34.0
5	29.068109891589792	34.0	23.0	34.0	11.0	34.0
6	31.499444481853075	37.0	27.0	38.0	11.0	38.0
7	32.526984714834015	37.0	31.0	38.0	12.0	38.0
8	31.714396336947008	37.0	27.0	38.0	11.0	38.0
9	33.1789946804929	37.0	34.0	38.0	21.0	38.0
10-11	32.99357787354387	38.0	34.0	38.0	11.0	38.0
12-13	31.95196451417413	37.0	30.0	38.0	16.0	38.0
14-15	32.003013265099995	37.0	29.0	38.0	11.5	38.0
16-17	31.696779678136153	36.5	29.0	38.0	11.0	38.0
18-19	32.233898390680764	37.0	29.5	38.0	11.5	38.0
20-21	31.222148340179114	36.5	27.0	38.0	11.0	38.0
22-23	31.148929365025925	36.0	28.5	38.0	11.0	38.0
24-25	32.42949128004848	37.0	30.5	38.0	11.0	38.0
26-27	30.935181132583665	36.5	25.5	38.0	11.0	38.0
28-29	33.02985489192647	37.5	34.0	38.0	11.0	38.0
30-31	33.47867146993468	38.0	34.0	38.0	11.0	38.0
32-33	33.56998686957107	38.0	34.0	38.0	11.0	38.0
34-35	33.631582048346914	38.0	34.0	38.0	16.0	38.0
36-37	31.56521446367248	37.0	27.5	38.0	11.0	38.0
38-39	29.768677193454984	35.0	23.0	38.0	11.0	38.0
40-41	31.03366776648037	36.5	26.5	38.0	11.0	38.0
42-43	32.08502794424618	37.0	30.0	38.0	16.0	38.0
44-45	33.62767658743519	38.0	34.5	38.0	21.0	38.0
46-47	32.252373577536865	37.5	30.0	38.0	16.0	38.0
48-49	31.616179045182143	36.5	28.5	38.0	11.0	38.0
50-51	29.504856575314793	35.0	23.0	38.0	11.0	38.0
52-53	32.78801932529797	37.0	32.5	38.0	11.0	38.0
54-55	31.90217830449128	37.0	29.5	38.0	11.0	38.0
56-57	32.31704094000404	37.5	30.0	38.0	15.5	38.0
58-59	33.35846070971652	38.0	34.0	38.0	11.0	38.0
60-61	33.79171436266918	38.0	35.0	38.0	21.0	38.0
62-63	33.69084573429399	38.0	35.0	38.0	16.0	38.0
64-65	33.39780991179045	38.0	35.0	38.0	16.0	38.0
66-67	31.47450508383274	37.0	27.0	38.0	11.0	38.0
68-69	31.503046932866475	37.0	27.0	38.0	11.0	38.0
70-71	30.976979664669045	36.5	25.5	38.0	11.0	38.0
72-73	29.89868527371894	35.0	23.5	38.0	11.0	38.0
74-75	31.928944178843174	36.5	30.0	38.0	11.0	38.0
76	32.493922968150294	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	17.0
13	118.0
14	359.0
15	616.0
16	906.0
17	1254.0
18	1501.0
19	1515.0
20	1275.0
21	947.0
22	716.0
23	564.0
24	549.0
25	562.0
26	637.0
27	801.0
28	848.0
29	1110.0
30	1536.0
31	2172.0
32	3069.0
33	4475.0
34	6718.0
35	9529.0
36	11512.0
37	6094.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.75994882499495	19.880816106659484	11.339303750589186	29.01993131775638
2	20.808363073193725	18.441519089623597	38.024375462931786	22.725742374250892
3	20.19729311157498	17.576257491078042	36.061544677126115	26.16490472022086
4	14.148878863376204	18.779880142751328	32.9977779274123	34.07346306646017
5	14.123628038515927	28.824658272170222	39.254932327789376	17.796781361524477
6	27.412295468318632	33.28058716584742	17.542589724597672	21.76452764123628
7	27.553700087536193	25.892195811729852	19.667025789509125	26.887078311224833
8	23.49000067335533	31.787421722442932	18.498754292640225	26.22382331156151
9	23.458016295198977	16.61504275806343	19.963302134536395	39.9636388122012
10-11	24.18018988620295	23.498417614975423	26.7843916234597	25.537000875361926
12-13	24.08043364813225	20.170698449573255	29.073447468983048	26.675420433311448
14-15	23.386472291428188	22.602013332435526	23.807319372432833	30.204195003703454
16-17	24.67847283011245	23.308194734361322	25.815601642987	26.197730792539225
18-19	22.389064709447176	23.362904854891926	27.537707898458013	26.71032253720288
20-21	24.344320247794762	22.72995084506094	27.35169348865396	25.574035418490336
22-23	24.823244225978048	22.0018853949229	27.302875227257424	25.871995151841627
24-25	22.328462729782505	24.943606491145378	24.547168540839	28.180762238233115
26-27	23.09291540060771	24.097064987753246	27.31741395709007	25.49260565454897
28-29	22.832829715420807	24.83355357849285	26.20131810415211	26.132298601934227
30-31	22.613797050703656	24.92929769039122	26.227190088209547	26.229715170695577
32-33	22.504376809642448	24.657430476062217	26.24402397144973	26.594168742845596
34-35	22.756043364083226	24.36788768433102	26.366911319103092	26.509157632482662
36-37	24.159350879998655	22.06267307481882	29.846726203001506	23.931249842181014
38-39	23.869604740421522	21.15850784458959	32.734327654703385	22.237559760285503
40-41	23.069153592350684	23.46390815433304	25.700289542791733	27.766648710524542
42-43	21.117264830651134	24.090128610867957	26.87950306376675	27.913103494714157
44-45	21.307487711265235	23.16931519762979	28.40886135613763	27.114335734967344
46-47	23.315769981819408	22.008618948218974	28.92566157161134	25.749949498350276
48-49	23.18951585751801	22.64578142886001	29.322099521917718	24.842603191704264
50-51	25.27355060265302	21.001952730455862	30.762238233115614	22.962258433775503
52-53	20.596256144367384	24.005117500505015	28.54521581038314	26.85341054474446
54-55	22.676082418692346	23.042219379166387	27.81041680694903	26.471281395192243
56-57	22.433674500033668	23.42687361120463	27.615985455524882	26.52346643323682
58-59	20.38330752137903	24.125479765672345	28.29775772675241	27.193454986196215
60-61	20.40014140461922	23.362904854891926	28.096592822032186	28.14036091845667
62-63	20.081981011379703	24.772742576257492	27.455221870581102	27.6900545417817
64-65	20.817621708975825	25.02861760150832	26.381220119857247	27.77254056965861
66-67	19.80590532624066	25.10352838192714	23.87212982290755	31.218436468924654
68-69	19.955047477944643	25.272745639436998	23.914068287426762	30.858138595191598
70-71	20.250319843781565	25.55383475860211	23.41845666958454	30.777388728031784
72-73	20.084506093865734	25.346777994747825	22.409265369335397	32.15945054205104
74-75	21.80156218436469	25.71123156689785	24.22564137095145	28.261564877786004
76	22.41936569927951	24.663322335196284	24.50845060938657	28.40886135613763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.5
16	3.0
17	4.0
18	7.5
19	12.5
20	20.5
21	27.0
22	35.5
23	53.0
24	76.5
25	91.0
26	121.5
27	187.0
28	252.5
29	283.0
30	358.0
31	496.0
32	699.5
33	840.0
34	934.0
35	1188.5
36	1515.5
37	1682.0
38	1882.0
39	2228.0
40	2556.5
41	2919.5
42	3100.0
43	3175.5
44	3326.5
45	3416.5
46	3431.0
47	3370.5
48	3205.0
49	2943.5
50	2787.0
51	2635.0
52	2345.5
53	2080.0
54	1952.0
55	1861.5
56	1617.5
57	1384.5
58	1305.0
59	1212.5
60	1064.5
61	989.5
62	970.0
63	942.0
64	911.5
65	887.0
66	838.5
67	812.0
68	817.5
69	803.0
70	737.5
71	692.0
72	658.5
73	590.5
74	510.5
75	465.0
76	426.0
77	331.0
78	253.5
79	232.0
80	197.0
81	126.0
82	75.0
83	60.0
84	49.5
85	32.0
86	17.5
87	10.0
88	7.5
89	4.0
90	2.5
91	2.0
92	2.0
93	2.5
94	1.5
95	0.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0016833883240185846
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.416941620092923E-4
28-29	8.416941620092923E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	8.416941620092923E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.013467106592148677
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	59404.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.55747087738199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.21926811834237	93.87246650057234
2	2.250736588852665	4.346508652615985
3	0.3591415470981014	1.040333984243485
4	0.1202949842221796	0.4646151774291294
5	0.029637894663435558	0.1430880075415797
6	0.01569065011593647	0.09090296949700358
7	0.0017434055684373857	0.011783718268130091
8	0.0017434055684373857	0.013467106592148677
9	0.0	0.0
>10	0.0017434055684373857	0.016833883240185844
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0016833883240185846	0.0
3	0.0	0.0	0.0	0.0016833883240185846	0.0
4	0.0	0.0	0.0	0.0016833883240185846	0.0
5	0.0	0.0	0.0	0.0016833883240185846	0.0
6	0.0	0.0	0.0	0.0016833883240185846	0.0
7	0.0	0.0	0.0	0.0016833883240185846	0.0
8	0.0	0.0	0.0	0.0016833883240185846	0.0
9	0.0	0.0	0.0	0.0016833883240185846	0.0
10	0.0	0.0	0.0	0.005050164972055754	0.0
11	0.0	0.0	0.0	0.005050164972055754	0.0
12	0.0	0.0	0.0	0.006733553296074338	0.0
13	0.0	0.0	0.0	0.006733553296074338	0.0
14	0.0	0.0	0.0	0.008416941620092924	0.0
15	0.0	0.0	0.0	0.008416941620092924	0.0
16	0.0	0.0	0.0	0.008416941620092924	0.0
17	0.0	0.0	0.0	0.008416941620092924	0.0
18	0.0	0.0	0.0	0.008416941620092924	0.0
19	0.0	0.0	0.0	0.011783718268130093	0.0
20	0.0	0.0	0.0	0.013467106592148677	0.0
21	0.0	0.0	0.0	0.016833883240185848	0.0
22	0.0	0.0	0.0	0.026934213184297354	0.0
23	0.0	0.0	0.0	0.028617601508315937	0.0
24	0.0	0.0	0.0	0.042084708100464614	0.0
25	0.0	0.0	0.0	0.0437680964244832	0.0
26	0.0	0.0	0.0	0.05050164972055754	0.0
27	0.0	0.0	0.0	0.08585280452494781	0.0
28	0.0	0.0	0.0	0.1195205710053195	0.0
29	0.0	0.0	0.0	0.13803784256952395	0.0
30	0.0	0.0	0.0	0.15823850245774695	0.0
31	0.0	0.0	0.0	0.17002222072587705	0.0
32	0.0	0.0	0.0	0.21715709379839743	0.0
33	0.0	0.0	0.0	0.23735775368662043	0.0
34	0.0	0.0	0.0	0.26597535519493637	0.0
35	0.0	0.0	0.0	0.3619284896639957	0.0
36	0.0	0.0	0.0	0.4359975759208134	0.0
37	0.0	0.0	0.0	0.6228536798868763	0.0
38	0.0	0.0	0.0	0.8080263955289206	0.0
39	0.0	0.0	0.0	1.0319170426233923	0.0
40	0.0	0.0	0.0	1.2827419029021614	0.0
41	0.0	0.0	0.0	1.7002222072587705	0.0
42	0.0	0.0	0.0	2.171570937983974	0.0
43	0.0	0.0	0.0	2.651336610329271	0.0
44	0.0	0.0	0.0	3.107534846138307	0.0
45	0.0	0.0	0.0	3.4139115211096898	0.0
46	0.0	0.0	0.0	3.797724058985927	0.0
47	0.0	0.0	0.0	4.2320382465827215	0.0
48	0.0	0.0	0.0	4.528314591609993	0.0
49	0.0	0.0	0.0016833883240185846	4.802706888425022	0.0
50	0.0	0.0	0.0016833883240185846	5.23365429937378	0.0
51	0.0	0.0	0.0016833883240185846	5.710053195071039	0.0
52	0.0	0.0	0.0016833883240185846	6.1662514308800755	0.0
53	0.0	0.0	0.0016833883240185846	6.821089488923305	0.0
54	0.0	0.0	0.0016833883240185846	7.472560770318497	0.0
55	0.0	0.0	0.0016833883240185846	7.853006531546697	0.0
56	0.0	0.0	0.0016833883240185846	8.240185846070972	0.0
57	0.0	0.0	0.0016833883240185846	8.576863510874688	0.0
58	0.0	0.0	0.0016833883240185846	8.831055147801495	0.0
59	0.0	0.0	0.0016833883240185846	9.142481987744933	0.0
60	0.0	0.0	0.0016833883240185846	9.295670325230624	0.0
61	0.0	0.0	0.0016833883240185846	9.5380782438893	0.0
62	0.0	0.0	0.0016833883240185846	9.70473368796714	0.0
63	0.0	0.0	0.0016833883240185846	10.001010032994412	0.0
64	0.0	0.0	0.0016833883240185846	10.282135883105514	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACA	15	0.0022145032	70.00001	56
ATAGGGT	15	0.0022145032	70.00001	6
ATCCCGG	15	0.0022145032	70.00001	42
CATCGGA	15	0.0022145032	70.00001	9
ACATCGG	15	0.0022145032	70.00001	8
GCTGTAG	15	0.0022145032	70.00001	51
TTCCGAC	20	7.866982E-5	70.0	19
GACGCTT	20	7.866982E-5	70.0	14
CGGTGGT	55	0.0	63.63636	46
TCTCGGT	40	1.0442818E-8	61.25	43
AGTGTAG	30	8.2830775E-6	58.333336	35
CTTCCGA	25	2.3725611E-4	55.999996	18
CTCGGTG	50	9.549694E-10	55.999996	44
TGTCGAT	25	2.3725611E-4	55.999996	37
AGTGTCG	25	2.3725611E-4	55.999996	35
CGACGAC	45	2.64572E-8	54.444443	22
TCGTTTT	20	0.0069197887	52.5	39
ACGCCGG	20	0.0069197887	52.5	29
TATATAG	20	0.0069197887	52.5	44
TTCCCGC	20	0.0069197887	52.5	52
>>END_MODULE