FastQCFastQC Report
Wed 25 May 2016
SRR1779686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59404
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT43627.342939869369067No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6951.1699548851929162Illumina PCR Primer Index 10 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG6571.1059861288802102No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3210.5403676520099656Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3190.5370008753619285Illumina PCR Primer Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2970.49996633223351966No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2520.4242138576526834No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT1470.24745808363073196Illumina PCR Primer Index 10 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1340.22557403541849033No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1260.2121069288263417No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1200.20200659888223016No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT940.15823850245774695No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC910.1531883374856912Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACA509.549694E-1055.9999963
CTATACA800.052.51
TACGTAG200.006919788752.54
GTGCAAA305.8342423E-446.6666681
TTTACAC602.5446752E-740.8333362
CTTTACA602.5446752E-740.8333361
TTGAGTA350.001246180940.00000445
GCCGTCT9200.039.94565646
TGCCGTC9150.039.7814245
ATCTCGT8900.039.719137
GTCTTCT9300.039.5161349
TCTCGTA8950.039.49720438
CTGCTTG9300.039.13978654
CCGTCTT9300.039.13978647
GTATGCC9300.039.13978642
ATGCCGT9300.039.13978644
GATTATC8800.038.97727233
CTCGTAT9250.038.97297339
TCGTATG9250.038.97297340
CGTATGC9350.038.93048541