FastQCFastQC Report
Wed 25 May 2016
SRR1779685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25127
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTG150.002199567470.030
AAGCCAT150.002199567470.025
CCCCGGT150.002199567470.043
CTCTGCC452.5638656E-854.44444316
CTTGTAC200.006873470752.537
GAGCTGT200.006873470752.556
GTGGCCG200.006873470752.528
ACCCCGG200.006873470752.542
TGACTCT200.006873470752.513
ACTCTGC352.02914E-550.015
TCTGCCG451.6203721E-646.66666417
CTGCCGC404.4670247E-543.7518
TGCCGCC450.00422942231.11110919
CTCATTC500.007078888428.0000028
TCTGACG1550.00711370613.54838759