##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779685_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25127
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.936761252835595	34.0	31.0	34.0	12.0	34.0
2	28.138416842440403	34.0	23.0	34.0	12.0	34.0
3	27.379790663429777	33.0	23.0	34.0	11.0	34.0
4	29.772754407609344	34.0	31.0	34.0	12.0	34.0
5	29.207267083217257	34.0	27.0	34.0	11.0	34.0
6	31.643292076252635	37.0	27.0	38.0	11.0	38.0
7	32.791021610220085	37.0	31.0	38.0	12.0	38.0
8	31.955506029370795	37.0	28.0	38.0	11.0	38.0
9	33.41401679468301	38.0	34.0	38.0	21.0	38.0
10-11	33.16908106817368	38.0	34.0	38.0	11.5	38.0
12-13	32.21144585505631	37.5	30.0	38.0	16.0	38.0
14-15	32.26980936840849	37.0	30.0	38.0	11.5	38.0
16-17	31.864269510884707	37.5	29.0	38.0	16.0	38.0
18-19	32.42396625144267	37.0	30.0	38.0	11.5	38.0
20-21	31.448879691168862	36.5	28.0	38.0	16.0	38.0
22-23	31.342340908186415	36.5	28.5	38.0	11.0	38.0
24-25	32.74841803637521	37.0	32.5	38.0	16.0	38.0
26-27	31.116348947347475	36.5	27.0	38.0	11.0	38.0
28-29	33.26929199665698	37.5	34.0	38.0	11.0	38.0
30-31	33.73311577187886	38.0	35.0	38.0	21.0	38.0
32-33	33.8116368846261	38.0	35.0	38.0	21.0	38.0
34-35	33.863752139133204	38.0	35.5	38.0	21.0	38.0
36-37	31.769510884705696	37.0	29.0	38.0	11.0	38.0
38-39	30.06305965694273	35.0	24.0	38.0	11.0	38.0
40-41	31.336749313487484	36.5	26.5	38.0	11.0	38.0
42-43	32.34379352887332	37.0	30.5	38.0	16.0	38.0
44-45	33.82878974807976	38.0	35.0	38.0	21.0	38.0
46-47	32.40522147490747	37.5	30.5	38.0	16.0	38.0
48-49	31.730509014207826	36.5	29.0	38.0	16.0	38.0
50-51	29.6848609065945	35.0	23.0	38.0	11.0	38.0
52-53	32.98278743980579	37.0	33.0	38.0	16.0	38.0
54-55	32.066203685278786	37.0	29.5	38.0	11.0	38.0
56-57	32.520117801568034	37.5	31.0	38.0	16.0	38.0
58-59	33.56071158514745	38.0	34.5	38.0	21.0	38.0
60-61	33.980598559318665	38.0	36.0	38.0	21.0	38.0
62-63	33.90567914991841	38.0	35.5	38.0	21.0	38.0
64-65	33.591196720659056	38.0	35.0	38.0	16.0	38.0
66-67	31.762705456282085	37.0	27.0	38.0	11.0	38.0
68-69	31.71677876387949	37.0	28.0	38.0	11.0	38.0
70-71	31.12404982687945	36.5	26.0	38.0	11.0	38.0
72-73	30.211107573526483	35.5	24.5	38.0	11.0	38.0
74-75	32.19116886218012	37.0	30.5	38.0	14.5	38.0
76	32.78552951008875	37.0	33.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	54.0
14	167.0
15	245.0
16	378.0
17	535.0
18	632.0
19	601.0
20	499.0
21	382.0
22	279.0
23	230.0
24	207.0
25	209.0
26	254.0
27	284.0
28	309.0
29	402.0
30	568.0
31	805.0
32	1214.0
33	1891.0
34	2804.0
35	4262.0
36	5178.0
37	2730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54893142834401	17.913001950093523	10.749393083137662	29.788673538424803
2	22.075854658335654	16.301189955028455	36.932383491861344	24.690571894774546
3	20.181478091296214	16.94193497035062	35.43598519520834	27.44060174314483
4	14.737135352409759	18.67314044653162	31.2412942253353	35.34842997572333
5	14.406813387989015	27.79082262108489	38.492458311776176	19.30990567914992
6	27.416723046921636	31.213435746408248	17.320014327217734	24.049826879452382
7	27.416723046921636	26.358100847693716	19.341743940780834	26.88343216460381
8	23.01906315915151	30.803518127910216	18.139849564213794	28.03756914872448
9	23.568273172284794	17.041429537947227	18.59354479245433	40.79675249731365
10-11	24.704501134238072	22.475822820074022	25.52035658853027	27.29931945715764
12-13	24.654753850439768	19.168623393162733	28.706172642973694	27.470450113423805
14-15	23.33545588410873	21.655987583077966	23.41107175548215	31.597484777331157
16-17	25.243761690611695	22.640983802284396	25.1820750587018	26.933179448402118
18-19	22.766346957456125	22.416126079516058	27.068492060333504	27.749034902694312
20-21	24.067735901619773	22.559398256855175	26.41381780554782	26.959048035977233
22-23	25.27361006089068	21.57838182035261	26.44167628447487	26.706331834281848
24-25	22.732518804473276	24.473673737413936	23.456839256576593	29.336968201536195
26-27	22.847932502885342	23.31953675329327	27.068492060333504	26.764038683487883
28-29	23.33545588410873	24.292593624388108	25.6397500696462	26.732200421856966
30-31	22.82007402395829	24.17717992597604	25.667608548573245	27.33513750149242
32-33	22.997174354280254	24.033907748636924	25.289529191706134	27.679388705376684
34-35	23.160345445138695	23.880686114538147	25.5840331117921	27.374935328531063
36-37	24.103553945954552	22.028097265889283	29.07430254308115	24.79404624507502
38-39	23.733434154495164	20.8421220201377	32.37752218728858	23.04692163807856
40-41	23.498626974967166	22.696700760138498	24.881601464560035	28.9230708003343
42-43	21.610220081983524	23.99410992159828	25.243761690611695	29.1519083058065
44-45	21.39730170732678	23.142436422971304	27.926135233016275	27.534126636685635
46-47	23.80905002586859	22.350459664902296	28.01170056114936	25.828789748079757
48-49	23.681696979344927	22.975285549409	27.812711425956145	25.530306045289926
50-51	25.15023679707088	20.871970390416685	29.985672782266086	23.992120030246348
52-53	21.33362518406495	23.57424284634059	27.55402555020496	27.538106419389504
54-55	23.28371870895849	22.19922792215545	27.364985871771403	27.152067497114658
56-57	23.078759899709475	23.003144028336052	26.89736140406734	27.02073466788713
58-59	21.456998447884747	23.958291877263502	27.09038086520476	27.494328809646994
60-61	21.8251283479922	23.202133163529272	26.316313129303143	28.65642535917539
62-63	20.977434632069087	23.91252437616906	26.75607911808015	28.353961873681698
64-65	21.57042225494488	25.136307557607356	25.70342659290803	27.589843594539737
66-67	20.52174951247662	24.774147331555696	23.12651729215585	31.577585863811837
68-69	20.567164179104477	24.955223880597018	23.592039800995025	30.88557213930348
70-71	20.736657778485295	25.349225932264098	23.21805229434473	30.696063994905877
72-73	20.24117483185418	24.51347156445258	22.179329008636127	33.06602459505711
74-75	21.480877144107932	25.239781907907826	24.286623950332313	28.99271699765193
76	23.317546861941338	23.767262307478013	24.45974449795041	28.455446332630242
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	1.0
19	3.0
20	5.5
21	7.0
22	6.5
23	12.0
24	24.5
25	31.0
26	42.5
27	70.5
28	109.5
29	132.0
30	152.0
31	196.0
32	257.5
33	295.0
34	355.0
35	469.5
36	579.0
37	634.0
38	700.5
39	837.0
40	982.5
41	1157.0
42	1256.0
43	1284.0
44	1294.5
45	1327.0
46	1377.0
47	1359.5
48	1304.0
49	1250.5
50	1235.0
51	1134.5
52	967.0
53	848.0
54	796.0
55	754.5
56	670.0
57	595.0
58	563.0
59	520.5
60	480.5
61	463.0
62	443.0
63	444.0
64	449.0
65	459.0
66	462.5
67	460.0
68	441.0
69	432.0
70	432.0
71	422.0
72	390.5
73	331.5
74	281.5
75	259.0
76	228.5
77	181.5
78	132.5
79	100.0
80	88.0
81	68.0
82	46.0
83	32.0
84	22.5
85	11.5
86	6.0
87	2.0
88	2.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.007959565407728738
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	25127.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.95439169021371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.2326412871247	96.22318621403272
2	1.5276479908991183	2.9927965933060054
3	0.1787673180839394	0.5253313169100967
4	0.04469182952098485	0.17511043897003226
5	0.012188680778450412	0.05969674055796554
6	0.004062893592816804	0.023878696223186214
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.003979782703864369	0.0
11	0.0	0.0	0.0	0.003979782703864369	0.0
12	0.0	0.0	0.0	0.003979782703864369	0.0
13	0.0	0.0	0.0	0.003979782703864369	0.0
14	0.0	0.0	0.0	0.007959565407728738	0.0
15	0.0	0.0	0.0	0.007959565407728738	0.0
16	0.0	0.0	0.0	0.007959565407728738	0.0
17	0.0	0.0	0.0	0.007959565407728738	0.0
18	0.0	0.0	0.0	0.007959565407728738	0.0
19	0.0	0.0	0.0	0.007959565407728738	0.0
20	0.0	0.0	0.0	0.015919130815457476	0.0
21	0.0	0.0	0.0	0.015919130815457476	0.0
22	0.0	0.0	0.0	0.0517371751502368	0.0
23	0.0	0.0	0.0	0.06765630596569427	0.0
24	0.0	0.0	0.0	0.07561587137342302	0.0
25	0.0	0.0	0.0	0.08357543678115174	0.0
26	0.0	0.0	0.0	0.08755521948501611	0.0
27	0.0	0.0	0.0	0.11143391570820234	0.0
28	0.0	0.0	0.0	0.1154136984120667	0.0
29	0.0	0.0	0.0	0.11939348111593107	0.0
30	0.0	0.0	0.0	0.1273530465236598	0.0
31	0.0	0.0	0.0	0.13133282922752418	0.0
32	0.0	0.0	0.0	0.15123174274684603	0.0
33	0.0	0.0	0.0	0.17113065626616786	0.0
34	0.0	0.0	0.0	0.19500935248935408	0.0
35	0.0	0.0	0.0	0.23878696223186213	0.0
36	0.0	0.0	0.0	0.30246348549369206	0.0
37	0.0	0.0	0.0	0.3860389222748438	0.0
38	0.0	0.0	0.0	0.5173717515023679	0.0
39	0.0	0.0	0.0	0.6885024077685359	0.0
40	0.0	0.0	0.0	0.8636128467385681	0.0
41	0.0	0.0	0.0	1.0626019819317865	0.0
42	0.0	0.0	0.0	1.2377124209018187	0.0
43	0.0	0.0	0.0	1.5083376447645958	0.0
44	0.0	0.0	0.0	1.7272256934771362	0.0
45	0.0	0.0	0.0	1.8903967843355753	0.0
46	0.0	0.0	0.0	2.0694870060094717	0.0
47	0.0	0.0	0.0	2.3799100569108926	0.0
48	0.0	0.0	0.0	2.662474628885263	0.0
49	0.0	0.0	0.0	2.8216659370398376	0.0
50	0.0	0.0	0.0	3.12412942253353	0.0
51	0.0	0.0	0.0	3.438532256138815	0.0
52	0.0	0.0	0.0	3.6415011740358976	0.0
53	0.0	0.0	0.0	3.987742269272098	0.0
54	0.0	0.0	0.0	4.2623472758387395	0.0
55	0.0	0.0	0.0	4.473275759143551	0.0
56	0.0	0.0	0.0	4.7279818521908705	0.0
57	0.0	0.0	0.0	4.958809249015004	0.0
58	0.0	0.0	0.0	5.145859036096629	0.0
59	0.0	0.0	0.0	5.384645998328491	0.0
60	0.0	0.0	0.0	5.54781708918693	0.0
61	0.0	0.0	0.0	5.786604051418792	0.0
62	0.0	0.0	0.0	5.933856011461774	0.0
63	0.0	0.0	0.0	6.1209057985434	0.0
64	0.0	0.0	0.0	6.256218410474788	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCTTG	15	0.0021995674	70.0	30
AAGCCAT	15	0.0021995674	70.0	25
CCCCGGT	15	0.0021995674	70.0	43
CTCTGCC	45	2.5638656E-8	54.444443	16
CTTGTAC	20	0.0068734707	52.5	37
GAGCTGT	20	0.0068734707	52.5	56
GTGGCCG	20	0.0068734707	52.5	28
ACCCCGG	20	0.0068734707	52.5	42
TGACTCT	20	0.0068734707	52.5	13
ACTCTGC	35	2.02914E-5	50.0	15
TCTGCCG	45	1.6203721E-6	46.666664	17
CTGCCGC	40	4.4670247E-5	43.75	18
TGCCGCC	45	0.004229422	31.111109	19
CTCATTC	50	0.0070788884	28.000002	8
TCTGACG	155	0.007113706	13.548387	59
>>END_MODULE