FastQCFastQC Report
Wed 25 May 2016
SRR1779685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25127
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT13605.412504477255542No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3641.4486409042066304RNA PCR Primer, Index 24 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG2210.8795319775540255No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2040.8118756715883312RNA PCR Primer, Index 24 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1580.6288056672105703No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1310.5213515342062323No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT760.302463485493692RNA PCR Primer, Index 24 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC710.2825645719743702No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG700.27858478927050584No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC540.2149082660086759No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT500.19898913519321845No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG410.16317109085843912No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT340.13531261193138855No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC290.1154136984120667No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT270.10745413300433795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT252.7608185E-670.000011
CAACTCG150.002199567470.036
GCTCTAC150.002199567470.01
TTTACAC308.144751E-658.3333322
ACTCTCC200.006873470752.534
CGCACTC200.006873470752.531
GGAACAA200.006873470752.517
CTTTACA352.02914E-550.01
TTGATTT305.7695835E-446.66666422
TTACACA404.4670247E-543.753
CTTTATA458.947249E-538.8888852
TTTATAC458.947249E-538.8888853
AGCATCT3250.034.4615434
GCATCTC3250.034.4615435
CCGTCTT3600.034.02777547
CGTCTTC3600.034.02777548
GTCTTCT3650.033.5616449
GCCGTCT3650.033.5616446
CTTGAAA3650.033.5616457
ATAGCAT3450.033.4782632