##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779684_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8642
Sequences flagged as poor quality	0
Sequence length	76
%GC	53
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.58643832446193	23.0	12.0	34.0	12.0	34.0
2	24.21164082388336	23.0	12.0	34.0	12.0	34.0
3	24.076486924323074	23.0	11.0	34.0	11.0	34.0
4	24.125896783152047	23.0	12.0	34.0	12.0	34.0
5	24.126475352927564	23.0	11.0	34.0	11.0	34.0
6	25.35119185373756	27.0	12.0	37.0	11.0	38.0
7	26.367970377227493	28.0	12.0	37.0	12.0	38.0
8	25.71985651469567	27.0	11.0	37.0	11.0	38.0
9	26.93959731543624	31.0	12.0	37.0	12.0	38.0
10-11	26.134691043739878	28.0	11.0	38.0	11.0	38.0
12-13	26.39956028697061	29.5	11.0	37.5	11.0	38.0
14-15	25.323825503355707	27.0	11.0	37.0	11.0	38.0
16-17	25.55768340661884	27.0	11.0	37.0	11.0	38.0
18-19	25.993288590604028	28.0	11.5	37.0	11.0	38.0
20-21	25.08638046748438	26.5	11.0	36.5	11.0	38.0
22-23	25.23108076834066	27.0	11.0	36.0	11.0	38.0
24-25	25.366871094654016	26.5	11.0	37.0	11.0	38.0
26-27	24.595174728072205	25.0	11.0	36.5	11.0	38.0
28-29	25.318329090488312	26.0	11.0	37.0	11.0	38.0
30-31	25.68016662809535	26.5	11.0	38.0	11.0	38.0
32-33	25.90048599861143	27.0	11.0	38.0	11.0	38.0
34-35	26.024126359638974	27.0	11.0	38.0	11.0	38.0
36-37	25.367507521407084	26.5	11.0	36.5	11.0	38.0
38-39	25.26434853043277	26.5	16.0	35.5	11.0	38.0
40-41	24.649386716037952	26.0	11.0	36.5	11.0	38.0
42-43	25.052707706549413	26.0	11.0	37.0	11.0	38.0
44-45	25.83875260356399	27.0	11.0	38.0	11.0	38.0
46-47	25.32376764637815	26.5	11.0	37.0	11.0	38.0
48-49	24.433464475815782	25.5	11.0	36.0	11.0	38.0
50-51	23.955045128442492	24.0	11.0	34.0	11.0	38.0
52-53	24.90210599398287	25.5	11.0	37.0	11.0	38.0
54-55	24.622251793566306	25.0	11.0	36.5	11.0	38.0
56-57	24.884806757694975	25.5	11.0	37.5	11.0	38.0
58-59	25.33018977088637	26.0	11.0	37.5	11.0	38.0
60-61	25.833545475584355	27.0	11.0	38.0	11.0	38.0
62-63	25.96464938671604	27.0	11.0	38.0	11.0	38.0
64-65	25.80392270307799	27.0	11.0	38.0	11.0	38.0
66-67	25.184795186299468	26.0	11.0	37.0	11.0	38.0
68-69	24.847489007174264	25.0	11.0	37.0	11.0	38.0
70-71	24.68768803517704	25.0	11.0	36.5	11.0	38.0
72-73	24.200300856283267	25.0	11.0	35.0	11.0	38.0
74-75	24.295591298310576	24.5	11.0	36.5	11.0	38.0
76	24.24149502429993	25.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	76.0
14	215.0
15	306.0
16	545.0
17	670.0
18	774.0
19	771.0
20	622.0
21	453.0
22	297.0
23	193.0
24	129.0
25	116.0
26	109.0
27	81.0
28	85.0
29	99.0
30	102.0
31	153.0
32	207.0
33	324.0
34	490.0
35	682.0
36	795.0
37	342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.16778523489933	18.224947928720205	17.646378153205276	38.96088868317519
2	14.313816246239297	12.601249710715113	51.86299467715807	21.221939365887525
3	14.61467252950706	12.84424901643138	47.801434853043276	24.739643601018283
4	12.994677158065263	26.162925248785	25.48021291367739	35.36218467947235
5	12.242536449895859	22.182365193242305	45.174728072205504	20.40037028465633
6	22.67993520018514	33.013191390881744	15.251099282573477	29.05577412635964
7	19.717657949548713	18.629946771580652	21.441795880583197	40.210599398287435
8	16.454524415644528	35.32747049294145	16.547095579726914	31.67090951168711
9	17.56537838463319	15.494098588289749	18.537375607498262	48.4031474195788
10-11	18.844017588521176	22.76672066651238	28.558204119416803	29.83105762554964
12-13	18.334876186068037	15.94538301319139	34.500115713955104	31.21962508678547
14-15	24.271002082851194	21.34922471650081	22.153436704466557	32.22633649618144
16-17	29.478130062485537	17.177736635038183	21.783152048137005	31.56098125433927
18-19	18.305947697292293	16.57602406850266	31.17912520249942	33.93890303170563
20-21	29.159916685952325	15.725526498495718	23.17750520712798	31.937051608423978
22-23	30.849340430455914	15.0890997454293	21.412867391807453	32.648692432307335
24-25	19.630872483221477	22.951862994677157	20.654940985882895	36.76232353621847
26-27	28.1937051608424	21.42443878731775	22.303864846100442	28.077991205739412
28-29	21.337653320990512	21.36079611201111	24.166859523258506	33.13469104373987
30-31	20.290442027308494	21.57486692895163	25.40499884286045	32.72969220087943
32-33	22.193936588752603	20.811154825271927	25.173570932654478	31.82133765332099
34-35	20.302013422818792	20.261513538532746	25.804211987965747	33.63226105068271
36-37	28.66813237676464	14.770886368896088	31.16176810923397	25.3992131451053
38-39	28.88798889146031	11.808609118259662	36.58296690580884	22.720435084471188
40-41	23.704003702846563	23.87178893774589	22.76672066651238	29.657486692895162
42-43	17.721592224022217	24.36935894468873	26.573709789400603	31.33533904188845
44-45	17.30502198565147	17.36866466095811	31.21962508678547	34.10668826660495
46-47	28.806989122888222	16.367738949317285	25.023142791020597	29.802129136773896
48-49	27.586206896551722	16.558666975237212	28.824346216153668	27.030779912057394
50-51	40.499884286044896	13.156676695209443	22.963434390187455	23.380004628558204
52-53	16.998380004628558	19.60194399444573	29.7789863457533	33.62068965517241
54-55	27.88706317981949	16.228882203193706	24.91321453367276	30.970840083314048
56-57	27.875491784309187	16.946308724832214	23.10807683406619	32.07012265679241
58-59	17.953020134228186	20.33094191159454	28.32677620921083	33.38926174496644
60-61	18.58944688729461	18.65308956260125	27.372136079611202	35.38532747049294
62-63	18.537375607498262	21.812080536912752	26.25549641286739	33.39504744272159
64-65	18.2596621152511	23.171719509372828	26.457995834297616	32.110622541078456
66-67	16.47766720666512	31.711409395973156	18.358018977088637	33.452904420273086
68-69	17.057223861598104	31.74796042353758	18.08135161719609	33.11346409766823
70-71	16.75538069891229	34.45961582966906	17.085165470955797	31.699838000462854
72-73	15.47095579726915	36.600323999074284	16.2751677852349	31.65355241842166
74-75	19.509372830363343	26.180282342050454	20.31358481832909	33.996760009257116
76	19.798657718120804	23.73293219162231	22.321221939365888	34.147188150891
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.5
21	1.0
22	1.5
23	4.0
24	8.0
25	10.0
26	10.5
27	14.0
28	26.0
29	35.0
30	35.0
31	47.0
32	71.5
33	84.0
34	90.5
35	114.0
36	157.5
37	184.0
38	190.5
39	205.0
40	245.5
41	280.0
42	282.0
43	320.0
44	352.0
45	368.5
46	391.0
47	379.0
48	381.0
49	390.0
50	385.0
51	356.5
52	313.0
53	315.5
54	333.0
55	309.0
56	274.5
57	275.5
58	287.0
59	285.5
60	280.5
61	256.5
62	236.0
63	247.0
64	251.5
65	241.5
66	236.0
67	234.0
68	228.5
69	212.0
70	188.0
71	175.0
72	157.0
73	126.5
74	107.5
75	101.0
76	88.5
77	68.5
78	47.0
79	33.0
80	31.0
81	22.0
82	11.5
83	8.0
84	7.0
85	6.0
86	4.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0057856977551492715
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	8642.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.57185836611896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.59325973271353	99.16685952325851
2	0.38349796629866356	0.7637121036797038
3	0.023242300987797792	0.06942837306179125
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.011571395510298541	0.0
19	0.0	0.0	0.0	0.023142791020597082	0.0
20	0.0	0.0	0.0	0.05785697755149271	0.0
21	0.0	0.0	0.0	0.09257116408238833	0.0
22	0.0	0.0	0.0	0.24299930571626938	0.0
23	0.0	0.0	0.0	0.24299930571626938	0.0
24	0.0	0.0	0.0	0.31242767877806066	0.0
25	0.0	0.0	0.0	0.31242767877806066	0.0
26	0.0	0.0	0.0	0.31242767877806066	0.0
27	0.0	0.0	0.0	0.31242767877806066	0.0
28	0.0	0.0	0.0	0.34714186530895624	0.0
29	0.0	0.0	0.0	0.34714186530895624	0.0
30	0.0	0.0	0.0	0.3587132608192548	0.0
31	0.0	0.0	0.0	0.3587132608192548	0.0
32	0.0	0.0	0.0	0.3587132608192548	0.0
33	0.0	0.0	0.0	0.3702846563295533	0.0
34	0.0	0.0	0.0	0.4165702383707475	0.0
35	0.0	0.0	0.0	0.4628558204119417	0.0
36	0.0	0.0	0.0	0.5091414024531359	0.0
37	0.0	0.0	0.0	0.6248553575561213	0.0
38	0.0	0.0	0.0	0.7058551261282111	0.0
39	0.0	0.0	0.0	0.8099976857208979	0.0
40	0.0	0.0	0.0	0.960425827354779	0.0
41	0.0	0.0	0.0	1.11085396898866	0.0
42	0.0	0.0	0.0	1.2959962971534367	0.0
43	0.0	0.0	0.0	1.388567461235825	0.0
44	0.0	0.0	0.0	1.5737097894006018	0.0
45	0.0	0.0	0.0	1.66628095348299	0.0
46	0.0	0.0	0.0	1.793566304096274	0.0
47	0.0	0.0	0.0	2.001851423281648	0.0
48	0.0	0.0	0.0	2.0597084008331406	0.0
49	0.0	0.0	0.0	2.1175653783846333	0.0
50	0.0	0.0	0.0	2.30270770654941	0.0
51	0.0	0.0	0.0	2.476278639203888	0.0
52	0.0	0.0	0.0	2.5919925943068733	0.0
53	0.0	0.0	0.0	2.8465632955334415	0.0
54	0.0	0.0	0.0	3.066419810229114	0.0
55	0.0	0.0	0.0	3.1474195788012036	0.0
56	0.0	0.0	0.0	3.2862763249247857	0.0
57	0.0	0.0	0.0	3.482990048599861	0.0
58	0.0	0.0	0.0	3.610275399213145	0.0
59	0.0	0.0	0.0	3.737560749826429	0.0
60	0.0	0.0	0.0	3.818560518398519	0.0
61	0.0	0.0	0.0	3.9342744735015045	0.0
62	0.0	0.0	0.0	3.9574172645221015	0.0
63	0.0	0.0	0.0	4.061559824114788	0.0
64	0.0	0.0	0.0	4.130988197176579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACTCT	20	7.490427E-5	70.00001	13
TTCCGAC	20	7.490427E-5	70.00001	19
CTGACTC	20	7.490427E-5	70.00001	12
TGCCTAC	25	2.6084563E-6	70.00001	19
GCTGCCT	20	7.490427E-5	70.00001	17
TCCACAT	20	7.490427E-5	70.00001	5
CTGCCTA	25	2.6084563E-6	70.00001	18
CTTCCGA	20	7.490427E-5	70.00001	18
ATCCACA	15	0.0021503684	70.0	4
GCCTACG	15	0.0021503684	70.0	20
GTATCCT	15	0.0021503684	70.0	57
TCTCGTT	15	0.0021503684	70.0	43
AGATCTC	15	0.0021503684	70.0	40
CTCGTTT	15	0.0021503684	70.0	44
TAGATCT	15	0.0021503684	70.0	39
CCACATC	30	7.697397E-6	58.333332	6
TCCGACG	30	7.697397E-6	58.333332	20
TATACCC	25	2.2596578E-4	56.000004	3
CTTATCC	25	2.2596578E-4	56.000004	1
TCTGACT	25	2.2596578E-4	56.000004	11
>>END_MODULE