FastQCFastQC Report
Wed 25 May 2016
SRR1779684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8642
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT190021.98565146956723No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5516.375838926174497TruSeq Adapter, Index 12 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2843.286276324924786No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2522.9159916685952325TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2142.476278639203888RNA PCR Primer, Index 12 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2032.348993288590604No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG840.9719972228650776No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC780.9025688498032862No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT770.8909974542929877RNA PCR Primer, Index 12 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC740.8562832677620921TruSeq Adapter, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG630.7289979171488081No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC570.6595695440870168No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT450.5207127979634344No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT430.4975700069428373No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT360.41657023837074747No Hit
CTTATAAACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT330.3818560518398519No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT260.3008562832677621No Hit
CTTATTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG250.2892848877574635No Hit
CTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT190.21985651469567227TruSeq Adapter, Index 12 (95% over 22bp)
CTTATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC190.21985651469567227No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG170.19671372367507522No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTTAAATCTCGTATGCCGT160.1851423281647767No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT160.1851423281647767No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGTCTT160.1851423281647767No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA150.17357093265447812No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCTGAAATCTCGTATGCCGT130.15042814163388105No Hit
CCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT120.1388567461235825No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGC110.12728535061328397No Hit
CTTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC110.12728535061328397No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGACATCTCGTATGCCGT90.10414255959268687No Hit
TCTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT90.10414255959268687No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGTC90.10414255959268687No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA150.002150368470.01
TCTTTAT353.1650416E-970.01
TTTATAC508.0035534E-1056.0000043
CTTTATA452.3066605E-854.4444432
AAAACCA200.006720885652.50000468
TTTACAC200.006720885652.5000042
CTTATAC3350.044.9253731
AACAAAA404.2240004E-543.75000470
TTATACA3950.038.101272
TATACAC4150.036.2650573
TTGAAAT4450.031.46067432
GAAATCT4350.031.3793134
TGAAATC4350.031.3793133
TCTTATA1257.2759576E-1130.8000011
AATCTCG4450.030.67415636
AAATCTC4450.030.67415635
ATCTCGT4500.030.33333437
AAACAAA703.1091837E-530.069
ACTACTT5050.029.80198127
ACTTGAA4950.029.6969730
Produced by FastQC (version 0.11.5)