##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779684_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8642
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.23906503124277	34.0	34.0	34.0	32.0	34.0
2	33.39817171950937	34.0	34.0	34.0	34.0	34.0
3	33.418421661652395	34.0	34.0	34.0	34.0	34.0
4	33.44758157833835	34.0	34.0	34.0	34.0	34.0
5	33.49456144411016	34.0	34.0	34.0	34.0	34.0
6	36.834066188382316	38.0	37.0	38.0	34.0	38.0
7	36.83938903031706	38.0	37.0	38.0	34.0	38.0
8	36.63469104373988	38.0	37.0	38.0	34.0	38.0
9	36.56248553575561	38.0	37.0	38.0	34.0	38.0
10-11	36.49919000231428	38.0	37.0	38.0	34.0	38.0
12-13	36.48194862300393	38.0	37.0	38.0	34.0	38.0
14-15	36.52840777597778	38.0	37.0	38.0	34.0	38.0
16-17	36.124161073825505	38.0	36.0	38.0	33.0	38.0
18-19	36.12057394121731	38.0	36.0	38.0	33.0	38.0
20-21	36.47691506595695	38.0	37.0	38.0	34.0	38.0
22-23	36.34916685952326	38.0	37.0	38.0	34.0	38.0
24-25	36.10448970145799	38.0	36.0	38.0	32.5	38.0
26-27	35.89736172182366	38.0	36.0	38.0	31.5	38.0
28-29	35.60558898403147	38.0	36.0	38.0	31.0	38.0
30-31	35.420678083776906	38.0	36.0	38.0	31.0	38.0
32-33	35.32272622078223	38.0	36.0	38.0	30.0	38.0
34-35	34.66217310807683	37.5	34.5	38.0	26.0	38.0
36-37	34.62508678546632	37.0	34.5	38.0	26.5	38.0
38-39	33.21088868317519	37.0	34.0	38.0	21.0	38.0
40-41	34.75057856977551	37.0	34.5	38.0	28.0	38.0
42-43	35.75271927794492	38.0	36.0	38.0	31.5	38.0
44-45	35.76579495487156	38.0	36.0	38.0	31.0	38.0
46-47	35.684853043277016	38.0	36.0	38.0	31.0	38.0
48-49	35.634343901874566	38.0	36.0	38.0	31.0	38.0
50-51	35.35934968757232	38.0	36.0	38.0	31.0	38.0
52-53	35.50150428141634	38.0	36.0	38.0	31.0	38.0
54-55	35.46679009488544	38.0	36.0	38.0	31.0	38.0
56-57	35.21383938903031	38.0	36.0	38.0	28.0	38.0
58-59	35.01267067808378	38.0	35.0	38.0	27.0	38.0
60-61	34.970377227493636	37.0	35.0	38.0	27.0	38.0
62-63	35.25908354547559	38.0	36.0	38.0	29.0	38.0
64-65	35.471823651932425	38.0	36.0	38.0	31.0	38.0
66-67	35.39574172645221	38.0	36.0	38.0	31.0	38.0
68-69	33.984494330016204	37.5	34.0	38.0	23.0	38.0
70-71	30.638509604258275	35.5	26.5	38.0	16.0	38.0
72-73	25.76510067114094	26.5	11.0	37.5	10.0	38.0
74-75	24.722749363573246	24.5	11.0	37.0	11.0	38.0
76	24.431613052534136	25.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	7.0
24	10.0
25	18.0
26	34.0
27	73.0
28	108.0
29	180.0
30	298.0
31	414.0
32	618.0
33	909.0
34	1467.0
35	1958.0
36	1326.0
37	1219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.930803054848415	14.68410090256885	14.59152973848646	51.793566304096274
2	10.367970377227493	9.638972460078685	59.88197176579495	20.11108539689887
3	11.640823883360333	19.463087248322147	54.17727377921777	14.718815089099746
4	9.176116639666745	38.96088868317519	27.93334876186068	23.929645915297385
5	9.592686878037492	33.64961814394816	46.0310113399676	10.726683638046747
6	16.57023837074751	47.107151122425364	13.260819254802128	23.061791252024992
7	14.579958342976163	31.97176579495487	12.161536681323767	41.286739180745194
8	12.855820411941679	48.634575329784774	13.839389030317056	24.67021522795649
9	11.791252024994215	19.91437167322379	8.123119648229576	60.17125665355242
10-11	14.313816246239297	29.62277250636427	30.479055774126362	25.584355473270076
12-13	16.564452672992363	9.864614672529507	33.04211987965749	40.52881277482064
14-15	20.880583198333717	11.235825040499885	12.890534598472575	54.99305716269382
16-17	36.536681323767645	27.192779449201577	6.902337421893081	29.3682018051377
18-19	35.22332793334876	17.675306641981024	7.081694052302708	40.01967137236751
20-21	19.411015968525806	12.630178199490857	7.30155056699838	60.657255264984954
22-23	20.79958342976163	30.426984494330018	7.289979171488081	41.48345290442027
24-25	36.27632492478593	33.46447581578339	7.023837074751214	23.235362184679474
26-27	34.20504512844249	36.69289516315668	11.762323536218467	17.339736172182366
28-29	16.396667438093036	18.780374913214533	29.379773200647996	35.443184448044434
30-31	17.183522332793334	26.492710020828515	22.541078454061562	33.782689192316596
32-33	17.21245082156908	19.312659106688265	43.75723212219393	19.717657949548713
34-35	31.312196250867853	34.9456144411016	20.13422818791946	13.607961120111087
36-37	16.35038185605184	50.387641749595005	15.731312196250869	17.53066419810229
38-39	16.153668132376765	17.28187919463087	30.16084239759315	36.40361027539921
40-41	17.36866466095811	11.559824114788244	33.29090488312891	37.78060634112474
42-43	35.29275630641055	12.115251099282572	33.406618838231886	19.185373756074984
44-45	19.949085859754685	25.983568618375376	33.39504744272159	20.672298079148348
46-47	35.17125665355242	10.234899328859061	18.143948160148113	36.44989585744041
48-49	34.01411710252256	7.30733626475353	16.790094885443185	41.88845174728072
50-51	18.334876186068037	8.105762554964128	35.50104142559592	38.058319833371904
52-53	15.656098125433926	7.677620921083082	52.7482064336959	23.918074519787087
54-55	16.83638046748438	7.498264290673455	40.08331404767415	35.58204119416802
56-57	33.97361721823652	7.990048599861144	23.50728997917149	34.52904420273085
58-59	19.312659106688265	15.436241610738255	45.770654940985885	19.480444341587596
60-61	30.750983568618373	38.538532747049295	17.391807451978707	13.31867623235362
62-63	14.620458227262208	63.77574635501041	8.157833834760472	13.445961582966905
64-65	11.58875260356399	66.6396667438093	8.036334181902337	13.735246470724368
66-67	11.530895626012496	66.6859523258505	7.845406155982411	13.937745892154593
68-69	12.132608192548021	65.08909974542931	8.128905345984725	14.649386716037954
70-71	12.526035639898172	59.22240222170794	10.466327239065032	17.78523489932886
72-73	13.984031474195788	48.25271927794492	13.775746355010416	23.987502892848877
74-75	14.747743577875491	42.970377227493636	16.390881740337885	25.890997454292986
76	14.68410090256885	44.11015968525804	15.667669520944225	25.538069891228883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	0.0
17	0.0
18	0.5
19	1.5
20	1.5
21	1.0
22	1.5
23	1.5
24	3.0
25	5.0
26	4.0
27	3.0
28	6.5
29	10.0
30	10.5
31	13.0
32	20.5
33	26.0
34	29.5
35	39.5
36	139.5
37	233.0
38	755.5
39	1411.5
40	1325.5
41	861.5
42	617.0
43	485.0
44	258.0
45	131.5
46	100.0
47	100.5
48	76.0
49	49.0
50	47.0
51	56.5
52	58.0
53	56.0
54	62.0
55	57.0
56	70.0
57	101.0
58	114.0
59	123.0
60	139.5
61	157.0
62	167.0
63	170.5
64	170.0
65	172.5
66	181.0
67	183.0
68	189.0
69	191.5
70	181.0
71	174.0
72	162.0
73	139.0
74	130.5
75	133.0
76	123.0
77	94.0
78	63.0
79	51.0
80	46.0
81	39.0
82	23.0
83	9.0
84	6.5
85	4.5
86	4.5
87	4.0
88	2.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	8642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.316593381161766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.98769483182936	35.96389724600787
2	10.527754990429314	8.909974542929877
3	1.9688269073010665	2.4994214302244853
4	0.6562756357670222	1.11085396898866
5	0.3828274541974296	0.8099976857208979
6	0.3007929997265518	0.7637121036797038
7	0.2461033634126333	0.7289979171488081
8	0.05468963631391851	0.1851423281647767
9	0.08203445447087777	0.31242767877806066
>10	0.46486190866830734	4.316130525341356
>50	0.16406890894175555	5.010414255959269
>100	0.10937927262783702	11.02753992131451
>500	0.027344818156959255	6.375838926174497
>1k	0.027344818156959255	21.98565146956723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	1900	21.98565146956723	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	551	6.375838926174497	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	284	3.286276324924786	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	252	2.9159916685952325	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	214	2.476278639203888	RNA PCR Primer, Index 12 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	203	2.348993288590604	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	84	0.9719972228650776	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	78	0.9025688498032862	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	77	0.8909974542929877	RNA PCR Primer, Index 12 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	74	0.8562832677620921	TruSeq Adapter, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	63	0.7289979171488081	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	57	0.6595695440870168	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	45	0.5207127979634344	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT	43	0.4975700069428373	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	36	0.41657023837074747	No Hit
CTTATAAACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	33	0.3818560518398519	No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	26	0.3008562832677621	No Hit
CTTATTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	25	0.2892848877574635	No Hit
CTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	19	0.21985651469567227	TruSeq Adapter, Index 12 (95% over 22bp)
CTTATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	19	0.21985651469567227	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG	17	0.19671372367507522	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTTAAATCTCGTATGCCGT	16	0.1851423281647767	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT	16	0.1851423281647767	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGTCTT	16	0.1851423281647767	No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA	15	0.17357093265447812	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCTGAAATCTCGTATGCCGT	13	0.15042814163388105	No Hit
CCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	12	0.1388567461235825	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGC	11	0.12728535061328397	No Hit
CTTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	11	0.12728535061328397	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGACATCTCGTATGCCGT	9	0.10414255959268687	No Hit
TCTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	9	0.10414255959268687	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGTC	9	0.10414255959268687	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.011571395510298541	0.0
20	0.0	0.0	0.0	0.05785697755149271	0.0
21	0.0	0.0	0.0	0.08099976857208979	0.0
22	0.0	0.0	0.0	0.10414255959268688	0.0
23	0.0	0.0	0.0	0.10414255959268688	0.0
24	0.0	0.0	0.0	0.10414255959268688	0.0
25	0.0	0.0	0.0	0.11571395510298542	0.0
26	0.0	0.0	0.0	0.1388567461235825	0.0
27	0.0	0.0	0.0	0.1388567461235825	0.0
28	0.0	0.0	0.0	0.16199953714417958	0.0
29	0.0	0.0	0.0	0.17357093265447812	0.0
30	0.0	0.0	0.0	0.17357093265447812	0.0
31	0.0	0.0	0.0	0.19671372367507522	0.0
32	0.0	0.0	0.0	0.19671372367507522	0.0
33	0.0	0.0	0.0	0.24299930571626938	0.0
34	0.0	0.0	0.0	0.31242767877806066	0.0
35	0.0	0.0	0.0	0.34714186530895624	0.0
36	0.0	0.0	0.0	0.4628558204119417	0.0
37	0.0	0.0	0.0	0.8447118722517936	0.0
38	0.0	0.0	0.0	0.9372830363341819	0.0
39	0.0	0.0	0.0	1.087711177968063	0.0
40	0.0	0.0	0.0	1.2959962971534367	0.0
41	0.0	0.0	0.0	1.4811386253182133	0.0
42	0.0	0.0	0.0	1.7009951400138856	0.0
43	0.0	0.0	0.0	1.8167090951168712	0.0
44	0.0	0.0	0.0	2.0365656098125435	0.0
45	0.0	0.0	0.0	2.22170793797732	0.0
46	0.0	0.0	0.0	2.4647072436935895	0.0
47	0.0	0.0	0.0	2.684563758389262	0.0
48	0.0	0.0	0.0	2.8118491090025457	0.0
49	0.0	0.0	0.0	2.9159916685952325	0.0
50	0.0	0.0	0.0	3.124276787780606	0.0
51	0.0	0.0	0.0	3.2978477204350845	0.0
52	0.0	0.0	0.0	3.436704466558667	0.0
53	0.0	0.0	0.0	3.6681323767646377	0.0
54	0.0	0.0	0.0	3.88798889146031	0.0
55	0.0	0.0	0.0	3.9689886600324	0.0
56	0.0	0.0	0.0	4.096274010645684	0.0
57	0.0	0.0	0.0	4.373987502892849	0.0
58	0.0	0.0	0.0	4.593844017588522	0.0
59	0.0	0.0	0.0	4.709557972691506	0.0
60	0.0	0.0	0.0	4.848414718815089	0.0
61	0.0	0.0	0.0	5.010414255959269	0.0
62	0.0	0.0	0.0	5.149271002082851	0.0
63	0.0	0.0	0.0	5.3112705392270305	0.0
64	0.0	0.0	0.0	5.461698680860912	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACA	15	0.0021503684	70.0	1
TCTTTAT	35	3.1650416E-9	70.0	1
TTTATAC	50	8.0035534E-10	56.000004	3
CTTTATA	45	2.3066605E-8	54.444443	2
AAAACCA	20	0.0067208856	52.500004	68
TTTACAC	20	0.0067208856	52.500004	2
CTTATAC	335	0.0	44.925373	1
AACAAAA	40	4.2240004E-5	43.750004	70
TTATACA	395	0.0	38.10127	2
TATACAC	415	0.0	36.265057	3
TTGAAAT	445	0.0	31.460674	32
GAAATCT	435	0.0	31.37931	34
TGAAATC	435	0.0	31.37931	33
TCTTATA	125	7.2759576E-11	30.800001	1
AATCTCG	445	0.0	30.674156	36
AAATCTC	445	0.0	30.674156	35
ATCTCGT	450	0.0	30.333334	37
AAACAAA	70	3.1091837E-5	30.0	69
ACTACTT	505	0.0	29.801981	27
ACTTGAA	495	0.0	29.69697	30
>>END_MODULE