FastQCFastQC Report
Wed 25 May 2016
SRR1779683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138719
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGG150.002220770870.03
GTGTATC252.3836886E-455.99999636
GCCGTAT509.713403E-1055.99999654
GTATTGT200.006939220252.522
GTATCTC407.4363015E-752.538
AAGGATC200.006939220252.543
TGCGTCA200.006939220252.569
TAGATCT506.14873E-848.99999639
GGTCGTA305.861442E-446.66666450
CATTGCG305.861442E-446.66666447
TGCCTAC659.915311E-943.07692319
TCGGTGG1450.041.0344845
CGGTGGT1550.040.6451646
CGTATCA701.9073923E-840.056
CGACTAG350.001251955540.022
GTAGATC459.160115E-538.88888538
ATCTCGG1051.2732926E-1136.66666442
TCCATAC501.7027553E-435.03
GCTGCCT708.626812E-735.017
CCGTATC1102.3646862E-1135.055
Produced by FastQC (version 0.11.5)