##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779683_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138719
Sequences flagged as poor quality	0
Sequence length	76
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.66893504134257	34.0	31.0	34.0	12.0	34.0
2	27.968692104181834	34.0	23.0	34.0	12.0	34.0
3	26.85518926751202	32.0	21.0	34.0	11.0	34.0
4	29.4548836136362	34.0	27.0	34.0	12.0	34.0
5	28.880369668178115	34.0	23.0	34.0	11.0	34.0
6	31.22625595628573	36.0	27.0	38.0	11.0	38.0
7	32.355012651475285	37.0	31.0	38.0	12.0	38.0
8	31.519532291899452	37.0	27.0	38.0	11.0	38.0
9	33.0334561235303	37.0	33.0	38.0	21.0	38.0
10-11	32.7625343319949	37.0	31.5	38.0	11.5	38.0
12-13	31.725080918980098	36.5	29.0	38.0	16.0	38.0
14-15	31.7516526214866	36.5	29.0	38.0	11.5	38.0
16-17	31.446344768921342	36.5	28.0	38.0	16.0	38.0
18-19	31.984573129852436	37.0	29.0	38.0	11.5	38.0
20-21	30.875222572250376	35.5	27.0	38.0	11.0	38.0
22-23	30.862217864892337	35.5	26.5	38.0	11.0	38.0
24-25	32.164465574290475	36.5	29.5	38.0	16.0	38.0
26-27	30.58374123227532	36.0	25.0	38.0	11.0	38.0
28-29	32.83778357687123	37.0	32.0	38.0	16.0	38.0
30-31	33.30678926462849	37.5	34.0	38.0	21.0	38.0
32-33	33.43616231374217	38.0	34.0	38.0	21.0	38.0
34-35	33.46642132656666	38.0	34.0	38.0	21.0	38.0
36-37	31.13007590885171	36.5	27.0	38.0	11.0	38.0
38-39	29.296383336096714	34.0	23.0	37.5	11.0	38.0
40-41	30.616790057598458	35.0	26.0	38.0	11.0	38.0
42-43	31.739343565048767	36.5	29.0	38.0	16.0	38.0
44-45	33.4448597524492	37.5	34.0	38.0	21.0	38.0
46-47	31.946889034667205	37.0	29.0	38.0	16.0	38.0
48-49	31.273452086592318	36.5	27.5	38.0	16.0	38.0
50-51	28.99176753004275	34.0	22.0	37.5	11.0	38.0
52-53	32.53716506030176	37.0	32.5	38.0	16.0	38.0
54-55	31.54185079188864	36.5	28.5	38.0	16.0	38.0
56-57	32.00012615431196	37.0	29.5	38.0	16.0	38.0
58-59	33.13483372861684	37.0	34.0	38.0	21.0	38.0
60-61	33.628075461905	38.0	34.0	38.0	21.0	38.0
62-63	33.48476055911591	38.0	34.0	38.0	20.0	38.0
64-65	33.16450882719742	38.0	33.0	38.0	16.0	38.0
66-67	31.01699478802471	36.5	26.0	38.0	11.0	38.0
68-69	31.09958260944788	36.5	26.0	38.0	11.0	38.0
70-71	30.580317043807987	36.0	25.5	38.0	11.0	38.0
72-73	29.290742436147895	34.0	22.5	37.5	11.0	38.0
74-75	31.53776339218132	36.5	28.0	38.0	14.5	38.0
76	31.976585759701265	37.0	30.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	5.0
12	29.0
13	199.0
14	466.0
15	868.0
16	1509.0
17	2253.0
18	2982.0
19	3155.0
20	2906.0
21	2433.0
22	1994.0
23	1911.0
24	1924.0
25	2078.0
26	2394.0
27	3003.0
28	3361.0
29	4288.0
30	5427.0
31	6886.0
32	8848.0
33	11644.0
34	15648.0
35	19781.0
36	21492.0
37	11233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.26241538650077	16.649485650848117	10.279053338043093	30.80904562460802
2	22.5585536227914	16.5918151082404	36.098876145300935	24.750755123667272
3	21.7208889914143	15.806053965210246	33.603904295734544	28.869152747640914
4	15.866722415259735	16.444873772689917	30.417105206245765	37.27129860580458
5	16.366899992070298	28.10573893987125	36.5350096237718	18.99235144428665
6	30.819858851346964	30.341914229485507	16.162890447595498	22.675336471572027
7	30.801337966233653	24.13962139015845	18.60248850185268	26.456552141755214
8	26.124411571761215	29.640202714879937	17.558770734661216	26.67661497869764
9	24.53123986245377	14.438028504076645	18.893863044904375	42.13686858856521
10-11	27.48611366429851	21.38621855524437	24.74417062260526	26.38349715785186
12-13	26.282381692156303	17.901619490111127	27.2696470859631	28.54635173176947
14-15	25.54805827692352	20.124425989604735	22.021814197250517	32.30570153622123
16-17	26.493264943463014	20.942295145785046	24.754081224385338	27.810358686366605
18-19	25.239967848093052	20.877112672248796	25.173645528830384	28.709273950827768
20-21	25.989604734819814	20.736823893250286	25.41937902347946	27.85419234845044
22-23	26.584171977277315	19.710415525245825	26.041336255370684	27.664076242106173
24-25	25.58013797876252	22.36712154962982	22.019651520722043	30.033088950885617
26-27	24.957737543839414	21.762731902837803	25.551033943330463	27.72849660999232
28-29	26.220024294153905	22.391352146283968	23.686800056229792	27.701823503332335
30-31	25.569693255956455	22.07259488880078	23.69066070720542	28.66705114803734
32-33	25.66412191728483	22.423711585458165	23.700051183344506	28.2121153139125
34-35	25.8533561135261	22.10832125838938	23.959932812849182	28.078389815235333
36-37	25.906888895296866	20.10474638830416	27.787349692897717	26.201015023501256
38-39	25.233031755758212	19.11184803373824	31.449014165735502	24.20610604476805
40-41	25.34792434875699	21.162018664100263	24.231250292865614	29.258806694277133
42-43	23.15317101303008	21.405734676590914	25.417124731919188	30.02396957845982
44-45	23.002393379278526	20.794645174312983	27.415006199717407	28.787955246691084
46-47	24.12826113598189	20.003316104010324	28.662312477922676	27.20611028208511
48-49	24.142405553773344	20.8136739320845	28.400730987301436	26.643189526840715
50-51	24.784001903162206	19.70890269001885	30.715163661136202	24.791931745682742
52-53	21.42875165356431	22.210207222696816	27.7820431026093	28.578998021129575
54-55	22.870304288587555	21.174765890265792	27.78318446909896	28.171745352047694
56-57	22.977994845639518	21.66309225584371	27.048137401553518	28.310775496963252
58-59	21.672337726001327	22.760171861933735	26.71177081230716	28.85571959975778
60-61	21.88644424579717	21.777228293780098	26.518209867643243	29.81811759277949
62-63	21.675942212866577	22.256625046858332	26.383041610196372	29.684391130078723
64-65	22.45126733758182	22.491998039159146	25.315032152022837	29.74170247123619
66-67	21.316007641567243	23.37094041740259	22.15549868435281	33.15755325667736
68-69	21.999783690244428	23.75081116158339	22.4233902949023	31.826014853269886
70-71	22.10583608779183	24.067779503945154	21.588226176787575	32.23815823147544
72-73	21.651870194751126	24.127801146951853	20.082110506756635	34.138218151540386
74-75	24.03579976354566	24.058147582110212	22.063424550880935	29.84262810346319
76	24.425268712558662	22.87390874946834	22.480301621286504	30.22052091668649
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.5
16	6.5
17	11.0
18	10.0
19	19.0
20	32.5
21	36.0
22	47.0
23	80.0
24	130.5
25	159.0
26	205.5
27	293.0
28	415.0
29	496.0
30	579.5
31	838.0
32	1102.0
33	1191.0
34	1418.5
35	1927.5
36	2497.0
37	2785.0
38	3137.5
39	3893.0
40	4606.5
41	5176.5
42	5436.0
43	5717.5
44	6066.0
45	6322.5
46	6512.0
47	6468.0
48	6278.0
49	5937.5
50	5743.0
51	5471.5
52	4951.5
53	4508.0
54	4313.0
55	4179.5
56	3879.0
57	3700.5
58	3689.0
59	3692.5
60	3818.5
61	3884.0
62	3827.0
63	3912.5
64	3973.0
65	3892.5
66	3758.5
67	3680.0
68	3556.5
69	3248.0
70	2912.5
71	2762.0
72	2549.0
73	2169.0
74	1885.5
75	1769.0
76	1563.0
77	1206.0
78	937.5
79	820.0
80	693.5
81	467.0
82	296.5
83	226.0
84	185.0
85	106.5
86	56.5
87	44.0
88	30.5
89	11.5
90	6.0
91	3.5
92	1.0
93	0.5
94	1.5
95	2.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	7.208817825964719E-4
5	0.0
6	0.0
7	7.208817825964719E-4
8	0.0014417635651929439
9	0.0014417635651929439
10-11	0.0025230862390876524
12-13	0.00180220445649118
14-15	0.0014417635651929439
16-17	0.003243968021684124
18-19	0.00180220445649118
20-21	0.0014417635651929439
22-23	0.002162645347789416
24-25	0.0014417635651929439
26-27	0.00180220445649118
28-29	0.00180220445649118
30-31	0.0028835271303858877
32-33	0.0014417635651929439
34-35	0.0014417635651929439
36-37	0.002162645347789416
38-39	0.0028835271303858877
40-41	0.0025230862390876524
42-43	0.001081322673894708
44-45	0.002162645347789416
46-47	0.0014417635651929439
48-49	0.00180220445649118
50-51	0.00180220445649118
52-53	0.0025230862390876524
54-55	0.0014417635651929439
56-57	0.001081322673894708
58-59	0.002162645347789416
60-61	0.002162645347789416
62-63	0.002162645347789416
64-65	0.002162645347789416
66-67	0.0028835271303858877
68-69	0.020905571695297685
70-71	0.003243968021684124
72-73	0.0025230862390876524
74-75	0.002162645347789416
76	0.0014417635651929439
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	138719.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.7583562328224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.16944697257294	91.1285871494059
2	2.868171589472244	5.435664504241426
3	0.5900859814803449	1.677467329233275
4	0.21352872376498633	0.8093452348985003
5	0.06494333638366752	0.30769619019957917
6	0.042925297568987934	0.24405183830652383
7	0.022954889846730275	0.15226173405671659
8	0.013971729067302692	0.10591504641183583
9	0.005987793651560767	0.051065413549490485
>10	0.007983686191249107	0.08794555969672133
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.20881782596472E-4	0.0
2	0.0	0.0	0.0	7.20881782596472E-4	0.0
3	0.0	0.0	0.0	7.20881782596472E-4	0.0
4	0.0	0.0	0.0	0.002162645347789416	0.0
5	0.0	0.0	0.0	0.002162645347789416	0.0
6	0.0	0.0	0.0	0.002162645347789416	0.0
7	0.0	0.0	0.0	0.002883527130385888	0.0
8	0.0	0.0	0.0	0.002883527130385888	0.0
9	0.0	0.0	0.0	0.002883527130385888	0.0
10	0.0	0.0	0.0	0.005046172478175304	0.0
11	0.0	0.0	0.0	0.005046172478175304	0.0
12	0.0	0.0	0.0	0.005046172478175304	0.0
13	0.0	0.0	0.0	0.005046172478175304	0.0
14	0.0	0.0	0.0	0.00720881782596472	0.0
15	0.0	0.0	0.0	0.00720881782596472	0.0
16	0.0	0.0	0.0	0.008650581391157664	0.0
17	0.0	0.0	0.0	0.010092344956350608	0.0
18	0.0	0.0	0.0	0.012975872086736496	0.0
19	0.0	0.0	0.0	0.01441763565192944	7.20881782596472E-4
20	0.0	0.0	0.0	0.015859399217122386	7.20881782596472E-4
21	0.0	0.0	0.0	0.016580280999718856	7.20881782596472E-4
22	0.0	0.0	0.0	0.027393507738665935	7.20881782596472E-4
23	0.0	0.0	0.0	0.02811438952126241	7.20881782596472E-4
24	0.0	0.0	0.0	0.030997916651648295	7.20881782596472E-4
25	0.0	0.0	0.0	0.033881443782034185	7.20881782596472E-4
26	0.0	0.0	0.0	0.041090261607998904	7.20881782596472E-4
27	0.0	0.0	0.0	0.09731904065052371	7.20881782596472E-4
28	0.0	0.0	0.0	0.13840930225852263	7.20881782596472E-4
29	0.0	0.0	0.0	0.17877868208392506	7.20881782596472E-4
30	0.0	0.0	0.0	0.2141018894311522	7.20881782596472E-4
31	0.0	0.0	0.0	0.2393327518220287	7.20881782596472E-4
32	0.0	0.0	0.0	0.27681860451704526	7.20881782596472E-4
33	0.0	0.0	0.0	0.3243968021684124	7.20881782596472E-4
34	0.0	0.0	0.0	0.3936014532976737	7.20881782596472E-4
35	0.0	0.0	0.0	0.58030983499016	0.001441763565192944
36	0.0	0.0	0.0	0.7259279550746474	0.001441763565192944
37	0.0	0.0	0.0	1.1743164238496528	0.001441763565192944
38	0.0	0.0	0.0	1.6306345922332197	0.001441763565192944
39	0.0	0.0	0.0	2.13525184005075	0.001441763565192944
40	0.0	0.0	0.0	2.742955182779576	0.001441763565192944
41	0.0	0.0	0.0	3.421304940202856	0.001441763565192944
42	0.0	0.0	0.0	4.071540308104874	0.001441763565192944
43	0.0	0.0	0.0	4.732588902745839	0.001441763565192944
44	0.0	0.0	0.0	5.40445072412575	0.001441763565192944
45	0.0	0.0	0.0	5.871582119248265	0.001441763565192944
46	0.0	0.0	0.0	6.472797525933722	0.001441763565192944
47	0.0	0.0	0.0	7.180703436443458	0.001441763565192944
48	0.0	0.0	0.0	7.625487496305481	0.001441763565192944
49	0.0	0.0	0.0	8.099106827471363	0.001441763565192944
50	0.0	0.0	0.0	8.760155422112328	0.001441763565192944
51	0.0	0.0	0.0	9.512756003143044	0.001441763565192944
52	0.0	0.0	0.0	10.129109927263029	0.001441763565192944
53	0.0	0.0	0.0	10.936497523771077	0.001441763565192944
54	0.0	0.0	0.0	11.644403434280813	0.001441763565192944
55	0.0	0.0	0.0	12.14757891853315	0.001441763565192944
56	0.0	0.0	0.0	12.616872959003453	0.001441763565192944
57	0.0	0.0	0.0	13.051564673909125	0.001441763565192944
58	0.0	0.0	0.0	13.4653508171195	0.001441763565192944
59	0.0	0.0	0.0	13.930319566894225	0.001441763565192944
60	0.0	0.0	0.0	14.250391078367059	0.001441763565192944
61	0.0	0.0	0.0	14.56685818092691	0.001441763565192944
62	0.0	0.0	0.0	14.810516223444518	0.001441763565192944
63	0.0	0.0	0.0	15.112565690352438	0.001441763565192944
64	0.0	0.0	0.0	15.40452281230401	0.001441763565192944
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAAGG	15	0.0022207708	70.0	3
GTGTATC	25	2.3836886E-4	55.999996	36
GCCGTAT	50	9.713403E-10	55.999996	54
GTATTGT	20	0.0069392202	52.5	22
GTATCTC	40	7.4363015E-7	52.5	38
AAGGATC	20	0.0069392202	52.5	43
TGCGTCA	20	0.0069392202	52.5	69
TAGATCT	50	6.14873E-8	48.999996	39
GGTCGTA	30	5.861442E-4	46.666664	50
CATTGCG	30	5.861442E-4	46.666664	47
TGCCTAC	65	9.915311E-9	43.076923	19
TCGGTGG	145	0.0	41.03448	45
CGGTGGT	155	0.0	40.64516	46
CGTATCA	70	1.9073923E-8	40.0	56
CGACTAG	35	0.0012519555	40.0	22
GTAGATC	45	9.160115E-5	38.888885	38
ATCTCGG	105	1.2732926E-11	36.666664	42
TCCATAC	50	1.7027553E-4	35.0	3
GCTGCCT	70	8.626812E-7	35.0	17
CCGTATC	110	2.3646862E-11	35.0	55
>>END_MODULE