FastQCFastQC Report
Wed 25 May 2016
SRR1779683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138719
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT112108.081084782906451TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG13991.0085136138524642TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC8420.6069824609462294RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT5180.3734167633849725TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC3930.2833065405604135RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3060.22058982547452044TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2450.17661603673613563RNA PCR Primer, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT2410.17373250960574976No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT150.002220770870.036
TCTTTAT850.053.5294081
AGAGTAC200.006939220252.528
CTGCATC200.006939220252.54
AGCGATC200.006939220252.533
AGTACTA200.006939220252.542
CGCTTTC200.006939220252.568
TCTCGTA17550.048.6609738
CTCGTAT18050.048.4764539
TCGTATG18050.048.4764540
CGTATGC18100.048.3425441
TATGCCG18150.048.20936643
CTTGAAA18250.047.7534257
TTGAAAA18250.047.7534258
GTATGCC18400.047.74456442
ATCTCGT17900.047.70949637
CCGTCTT18000.047.6388947
GCCGTCT18150.047.63085646
CTGCTTG18450.047.42547254
TCTTCTG18100.047.3756950
Produced by FastQC (version 0.11.5)