FastQCFastQC Report
Wed 25 May 2016
SRR1779682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465162
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223614.807142457896388No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG200.006949447652.5000041
CATTGAT552.9907355E-431.81818430
TGTACAC552.9907355E-431.8181843
CTAATCG657.9521735E-426.92307926
ACGCGAG700.001225456825.019
CACATTG851.3073838E-424.70588328
GCTCTAG851.3073838E-424.7058831
TAGACCG750.001831398523.3333345
TACCGAC901.926472E-423.33333265
GTTAACT800.00266478321.87500211
CGAGAAT800.00266478321.87500214
AAGCACA1304.335723E-621.5384644
TTGTGGA1003.928778E-421.02
TAGACTG850.003787422620.5882365
CTATCCA850.003787422620.5882364
ACATTGA850.003787422620.58823629
AATCGTC850.003787422620.58823628
TACCGCT850.003787422620.58823625
TAATCGT850.003787422620.58823627
CGAGTAC1055.459844E-420.01
Produced by FastQC (version 0.11.5)