FastQCFastQC Report
Wed 25 May 2016
SRR1779682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465162
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT140473.019808152858574No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG19280.4144792566890675No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC8180.17585271367824543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG15900.058.1047370
CGTATGC24950.053.46208641
TCGTATG25800.053.3119140
CTCGTAT25300.052.98215539
CCGTCTT23950.052.9339947
GTATGCC24950.052.4911342
TCTCGTA25200.052.359138
TATGCCG25050.052.14179243
GTCTTCT24850.051.986649
CTTGAAA25700.051.74898557
CGTCTTC24950.051.3738548
TTGAAAA26350.051.13655558
ATCTCGT25700.051.06807737
CTGCTTG24750.051.06501454
GCCGTCT25100.050.9273146
TTCTGCT24900.050.6167952
CTTCTGC25400.050.3095751
TGCTTGA25700.050.26179555
AATCTCG26200.050.2216836
ATGCCGT26000.050.1019344