FastQCFastQC Report
Wed 25 May 2016
SRR1779681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549016
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG249734.548683462777041No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG200.00695011252.512
TACCAAG400.00241833635.021
CTGTTTA400.00241833635.050
CTATATG450.0043065231.1111131
CATAACG450.0043065231.1111134
ATAACGG450.0043065231.11111342
CGTTTAC450.0043065231.11111314
GCCTTAC604.980942E-429.1666661
TTACGTT604.980942E-429.16666652
TAGTGTC500.007207141727.99999845
TATAGTT1154.8947186E-827.3913042
TAGAACA1358.069037E-925.9259264
TTCCGTT700.001225738425.021
GGTATTA700.001225738425.036
GTACATG901.9270871E-423.3333341
CTTAGGT5250.023.33333470
CTATAGT1052.0381136E-523.3333321
GGCTTAG11550.022.72727470
CTCCTAA1102.9198713E-522.2727261
GAATATG952.7788428E-422.1052651
Produced by FastQC (version 0.11.5)