FastQCFastQC Report
Wed 25 May 2016
SRR1779681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549016
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT151532.7600288516181677No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG20330.37029886196395007No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC11280.2054584930129541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG17000.057.02318270
CGTATGC27450.052.67281741
TCGTTAG200.00695311752.4942639
TCGTATG28650.051.91404340
CTCGTAT28600.051.5153539
CTTGAAA27700.051.42037257
CCGTCTT26600.051.35227647
TCTCGTA29500.051.24863438
GTATGCC27800.051.14212442
TGAAAAA30350.050.85111259
TCTTCTG26950.050.7875550
GTCTTCT27050.050.72920249
TTGAAAA28650.050.6925458
GCTTGAA28300.050.45844356
GCCGTCT27350.050.07213646
TATGCCG28350.050.03098743
GTATCTC29050.049.99453735
GAAAAAA32250.049.48296460
CGTATCT29350.049.3642834
TCTGCTT27550.049.17321453
Produced by FastQC (version 0.11.5)