FastQCFastQC Report
Wed 25 May 2016
SRR1779680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332684
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161374.850548869197196No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGAT350.001254483140.032
TTAGAGT601.2139542E-535.04
AGTGTCC400.00241697135.020
CGGAAAT450.00430410431.11111355
GCGAATA450.00430410431.11111357
TGTGCGC450.00430410431.1111133
CGAATAG450.00430410431.11111358
TTACGTC450.00430410431.11111338
CGCAATA450.00430410431.11111357
GCCGTAT450.00430410431.11111354
GATAGCG450.00430410431.11111346
GTTGCAC450.00430410431.11111339
TCGGAAA500.007203120328.00000254
GCGAAAT657.9473876E-426.92307711
CATGTAA808.6525084E-526.2533
CAGCGAA700.001224722825.025
TTTCCCA700.001224722825.029
GTACTGA700.001224722825.01
GTAGATC1001.3948806E-524.50000238
ACAGTTA750.001830306623.33333451
Produced by FastQC (version 0.11.5)