##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 332684 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.372662947421578 32.0 27.0 32.0 13.0 32.0 2 29.193057075182455 32.0 32.0 32.0 22.0 32.0 3 28.77537543134025 32.0 27.0 32.0 22.0 32.0 4 27.70101056858761 32.0 27.0 32.0 13.0 32.0 5 28.688313835351263 32.0 27.0 32.0 13.0 32.0 6 32.52012119608998 37.0 32.0 37.0 13.0 37.0 7 32.28093025213115 37.0 32.0 37.0 13.0 37.0 8 32.47814743119597 37.0 32.0 37.0 13.0 37.0 9 32.55805208546248 37.0 32.0 37.0 13.0 37.0 10-11 32.17182220966443 37.0 32.0 37.0 13.0 37.0 12-13 32.09011253922641 37.0 32.0 37.0 13.0 37.0 14-15 33.05960160392445 37.0 34.5 37.0 17.5 37.0 16-17 33.09660218104868 37.0 34.5 37.0 17.5 37.0 18-19 32.339371595868755 37.0 32.0 37.0 13.0 37.0 20-21 32.87314388428659 37.0 34.5 37.0 17.5 37.0 22-23 32.74632534176576 37.0 34.5 37.0 17.5 37.0 24-25 32.05614186435176 37.0 32.0 37.0 13.0 37.0 26-27 32.6635020018997 37.0 34.5 37.0 17.5 37.0 28-29 32.17082125981412 37.0 32.0 37.0 13.0 37.0 30-31 32.38310078032006 37.0 32.0 37.0 13.0 37.0 32-33 32.348099097041036 37.0 32.0 37.0 13.0 37.0 34-35 32.295514662562674 37.0 32.0 37.0 13.0 37.0 36-37 33.03306290654194 37.0 34.5 37.0 17.5 37.0 38-39 32.896941241538514 37.0 34.5 37.0 17.5 37.0 40-41 32.25275636940761 37.0 32.0 37.0 13.0 37.0 42-43 32.245504743239835 37.0 32.0 37.0 13.0 37.0 44-45 31.86188244700677 37.0 32.0 37.0 13.0 37.0 46-47 32.24434147719758 37.0 32.0 37.0 13.0 37.0 48-49 32.77071034374963 37.0 32.0 37.0 13.0 37.0 50-51 33.02864580202234 37.0 32.0 37.0 13.0 37.0 52-53 31.920475586442386 37.0 32.0 37.0 13.0 37.0 54-55 32.10356674802515 37.0 32.0 37.0 13.0 37.0 56-57 31.8572910028736 37.0 32.0 37.0 13.0 37.0 58-59 31.395786993062487 37.0 29.5 37.0 13.0 37.0 60-61 31.190862800735832 37.0 29.5 37.0 13.0 37.0 62-63 30.861629955152637 37.0 27.0 37.0 13.0 37.0 64-65 30.848117132173474 37.0 27.0 37.0 13.0 37.0 66-67 30.922179906457778 37.0 27.0 37.0 13.0 37.0 68-69 31.24654326628272 37.0 27.0 37.0 13.0 37.0 70-71 31.33520097149247 37.0 29.5 37.0 13.0 37.0 72-73 31.59797285111397 37.0 32.0 37.0 13.0 37.0 74-75 32.017668418078415 37.0 32.0 37.0 13.0 37.0 76 31.60581512786909 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.16886828462108738 11101 2 0.2421224861090927 11101 3 -0.41245614948628173 11101 4 0.029852128001525813 11101 5 -0.3109817622653601 11101 6 -0.25781231947635774 11101 7 0.14914357807809608 11101 8 -0.856403910335704 11101 9 0.4865217630280654 11101 10-11 0.13624758503560486 11101 12-13 0.43315288589803913 11101 14-15 0.49738414614053994 11101 16-17 0.42723110589272295 11101 18-19 -0.10087480936452664 11101 20-21 0.8252524490950179 11101 22-23 0.2282929691379536 11101 24-25 0.7748154912503082 11101 26-27 0.38443761298330514 11101 28-29 0.28330433204043004 11101 30-31 0.6898966536309459 11101 32-33 0.7668946435016721 11101 34-35 0.4397856955001629 11101 36-37 -0.3985863122805 11101 38-39 0.24929311438332036 11101 40-41 0.4882346293053814 11101 42-43 0.7561090152338537 11101 44-45 0.43066154714637506 11101 46-47 -0.21065649426747513 11101 48-49 0.39801792292545457 11101 50-51 -0.03320500066084264 11101 52-53 0.3976616147778813 11101 54-55 -0.3682193330054844 11101 56-57 -0.6158440195100745 11101 58-59 0.7941953645117863 11101 60-61 0.5673857539377103 11101 62-63 0.21644029715913504 11101 64-65 0.02408700727114166 11101 66-67 -0.08778541117891336 11101 68-69 -0.8516308420499819 11101 70-71 0.4030831938376096 11101 72-73 0.391262647390235 11101 74-75 -0.8146061972606589 11101 76 -1.3195901824137444 11102 1 -0.020472528843150428 11102 2 -0.41653810295121474 11102 3 0.4827635793589735 11102 4 0.564856803083341 11102 5 0.5842379665798951 11102 6 -0.04842709273489021 11102 7 -0.29691766549366605 11102 8 0.23195513594760797 11102 9 -1.1162715983557625 11102 10-11 -0.23252871229959737 11102 12-13 -0.17235786679013643 11102 14-15 0.5305772270194495 11102 16-17 -0.5590358412788561 11102 18-19 0.015242535189010198 11102 20-21 0.5956182742469593 11102 22-23 -0.8324246559204838 11102 24-25 -0.9856450043083598 11102 26-27 -0.11296187275769398 11102 28-29 -1.0140016520642945 11102 30-31 0.23954017864262767 11102 32-33 0.3138207772090169 11102 34-35 0.444227023223398 11102 36-37 -0.022505199611032367 11102 38-39 -0.19253133629456443 11102 40-41 1.0481633343077164 11102 42-43 -0.17128112034352938 11102 44-45 0.4692601913726584 11102 46-47 -0.4851130160066077 11102 48-49 -0.4837609457047449 11102 50-51 0.2675780755429855 11102 52-53 -0.5042493249135624 11102 54-55 0.13987440238488347 11102 56-57 -0.048318306560094726 11102 58-59 -0.8450344157593719 11102 60-61 -0.3972297205830948 11102 62-63 -0.1471641909194048 11102 64-65 -0.38536250184526466 11102 66-67 0.6980374032203365 11102 68-69 0.059717919053337454 11102 70-71 0.12506192221535528 11102 72-73 -0.19914174718666544 11102 74-75 0.0973561683307409 11102 76 -0.06631762514399142 11103 1 -0.16315809213885046 11103 2 0.5516038619779238 11103 3 0.5367210619436484 11103 4 0.9116319652260252 11103 5 0.4558750071756208 11103 6 0.6776418479328967 11103 7 0.42158461651450096 11103 8 0.7189837211025392 11103 9 1.0788701263619913 11103 10-11 0.5015122437417219 11103 12-13 0.8321310834861038 11103 14-15 0.7557754014129188 11103 16-17 0.17069632302573012 11103 18-19 0.5588709281748336 11103 20-21 0.06347498815519259 11103 22-23 0.0601708281054556 11103 24-25 0.868396552261423 11103 26-27 0.7791682017078401 11103 28-29 -0.07863352811521906 11103 30-31 0.08125588846728249 11103 32-33 0.2798008396639773 11103 34-35 0.5159901747967837 11103 36-37 0.4991888911888722 11103 38-39 0.475871858516868 11103 40-41 0.7441307856649075 11103 42-43 0.4917473447057219 11103 44-45 0.773232687168953 11103 46-47 0.7921059366717529 11103 48-49 0.4622815717105837 11103 50-51 0.00884038637652651 11103 52-53 -0.40968110487993314 11103 54-55 -0.41394796129515044 11103 56-57 -0.48843060538306204 11103 58-59 0.6585837682425506 11103 60-61 -1.4528628090815232E-4 11103 62-63 0.4636333108821802 11103 64-65 0.8564252713951888 11103 66-67 0.38999632707332665 11103 68-69 0.5363273353396671 11103 70-71 0.43269764164845625 11103 72-73 -0.06363249904830326 11103 74-75 -0.4568565388515822 11103 76 0.4345831675535834 11104 1 0.3904830103364141 11104 2 -0.0029112299470384073 11104 3 0.37422145211751 11104 4 -0.3628943637061468 11104 5 0.3005545964005769 11104 6 0.670837215788815 11104 7 0.7319757267932445 11104 8 0.05693671295571079 11104 9 -0.11581102355841466 11104 10-11 0.6554748494788356 11104 12-13 0.22912518405732385 11104 14-15 -0.279543136527586 11104 16-17 -0.004288850367530017 11104 18-19 -0.03508293766778792 11104 20-21 -0.23908143098516632 11104 22-23 0.46120712988152945 11104 24-25 0.9109098962074853 11104 26-27 0.7737213746016778 11104 28-29 0.4447769035731426 11104 30-31 0.6029183412562418 11104 32-33 0.14036830852810311 11104 34-35 0.7698841099342815 11104 36-37 0.31268578540493763 11104 38-39 -0.18336589782736468 11104 40-41 0.5553022436665955 11104 42-43 0.9192218273351784 11104 44-45 0.06461452691674552 11104 46-47 -0.03269039257255457 11104 48-49 0.4750636674124209 11104 50-51 0.3074638529932514 11104 52-53 0.15454151271846683 11104 54-55 0.5933717003460757 11104 56-57 0.6407169427643957 11104 58-59 0.14288317205689083 11104 60-61 0.24421279968219523 11104 62-63 0.09460687270132695 11104 64-65 1.552198908127714 11104 66-67 1.0713179239769097 11104 68-69 0.5641701420943583 11104 70-71 -0.5457959751071684 11104 72-73 0.808749434341216 11104 74-75 0.2325962813250939 11104 76 -0.3720778806094778 11105 1 0.9111397899974314 11105 2 0.5836883463241485 11105 3 0.11795379675027817 11105 4 0.34588035380797777 11105 5 0.38609485063786764 11105 6 0.2339551536419222 11105 7 0.12427798668024792 11105 8 -0.03751950173355567 11105 9 0.6414276770065541 11105 10-11 -0.0639372267923548 11105 12-13 -0.14892036679013643 11105 14-15 -0.24648346138634025 11105 16-17 -0.0679805876556685 11105 18-19 0.6251836583774093 11105 20-21 0.2693048684498578 11105 22-23 0.254418641180969 11105 24-25 -0.2914647506851722 11105 26-27 -0.14908053217798312 11105 28-29 0.7682673696748381 11105 30-31 -0.36428681411099006 11105 32-33 -0.12322632424026381 11105 34-35 -0.7604831217041337 11105 36-37 -0.03190284453857117 11105 38-39 0.08724674341557659 11105 40-41 -0.22833123090967433 11105 42-43 0.3051681550187908 11105 44-45 0.3795862783291746 11105 46-47 -0.392948160934143 11105 48-49 0.3680280035706218 11105 50-51 -0.14014384474685926 11105 52-53 -0.3010111002758826 11105 54-55 -0.7508638222527964 11105 56-57 -0.6485221109079227 11105 58-59 -0.1779148505419812 11105 60-61 0.05159274318502227 11105 62-63 -0.295488466281725 11105 64-65 -0.10082898735251433 11105 66-67 0.26755733075656707 11105 68-69 0.2365748031113064 11105 70-71 -1.0215639835817463 11105 72-73 -0.4217413848678291 11105 74-75 -0.13181049833592695 11105 76 -0.8342071178976163 11106 1 -0.046697511589872676 11106 2 0.03466387542467686 11106 3 0.06330763272911355 11106 4 -0.0874033763507498 11106 5 -0.02040316523514818 11106 6 0.267106608668378 11106 7 -0.10158180167954356 11106 8 -0.1256478086118591 11106 9 -0.3025207356918571 11106 10-11 -0.16781454160716436 11106 12-13 0.6494013924691231 11106 14-15 -0.5696068079471956 11106 16-17 -0.19996636807893964 11106 18-19 0.8750596504409032 11106 20-21 0.8127076462276364 11106 22-23 0.08680124700106973 11106 24-25 0.7755408710079479 11106 26-27 0.21794162390667537 11106 28-29 -0.3129578287378578 11106 30-31 -0.23445050458717986 11106 32-33 -0.13628849355242778 11106 34-35 0.6250988888778721 11106 36-37 -0.4742391540623814 11106 38-39 0.08894151854784837 11106 40-41 0.27241281670937667 11106 42-43 -0.2269085645579274 11106 44-45 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11108 5 -0.07221204445519547 11108 6 0.21986357157761915 11108 7 0.4786561592690788 11108 8 0.47395469454394146 11108 9 0.144018624553091 11108 10-11 0.38980907185401037 11108 12-13 0.18455837686468612 11108 14-15 -0.04115086832374004 11108 16-17 0.7691201949162476 11108 18-19 0.7975477658224221 11108 20-21 0.1838300376545945 11108 22-23 0.7077154897427249 11108 24-25 0.4744907273182122 11108 26-27 -0.376383302905559 11108 28-29 0.30423659556316096 11108 30-31 0.8942659566165858 11108 32-33 0.49410658608123015 11108 34-35 0.5781378596833378 11108 36-37 -0.5386578149277383 11108 38-39 0.07607658690119479 11108 40-41 0.6410374239126639 11108 42-43 0.13818422946888376 11108 44-45 0.6042055676692719 11108 46-47 -0.10405750949590242 11108 48-49 0.06101393842678959 11108 50-51 -0.07199928044906301 11108 52-53 0.199610198370479 11108 54-55 0.5064463511820563 11108 56-57 0.15402389163014973 11108 58-59 1.319071190067156 11108 60-61 0.015055095130875884 11108 62-63 0.45485523084179746 11108 64-65 0.35841646527016024 11108 66-67 -0.06368738159537912 11108 68-69 0.44641511614007356 11108 70-71 1.1447457668412682 11108 72-73 0.8950151718157642 11108 74-75 0.4307952546251599 11108 76 0.7092837873477293 11109 1 0.4776462002538402 11109 2 0.343704371965174 11109 3 -0.31113274171126193 11109 4 0.23730663585926237 11109 5 0.3288031839711998 11109 6 0.2287468203085865 11109 7 0.5088933712956347 11109 8 0.37994844698439323 11109 9 0.2560110103398898 11109 10-11 -0.3041215216641433 11109 12-13 0.4189882870560169 11109 14-15 0.41237070528032405 11109 16-17 0.8320594764468936 11109 18-19 0.6009448763261318 11109 20-21 -0.06763423411424441 11109 22-23 0.506622166821991 11109 24-25 -0.2171458404287563 11109 26-27 -0.10240585269080071 11109 28-29 0.0633394850594513 11109 30-31 -0.06721149359817247 11109 32-33 -0.2450211960351325 11109 34-35 -0.6370856858067029 11109 36-37 -0.49083713941035967 11109 38-39 0.02695026905660569 11109 40-41 -0.34972546167890783 11109 42-43 0.3981168729675133 11109 44-45 0.3098747398676345 11109 46-47 0.14992042880944467 11109 48-49 0.4663853753654905 11109 50-51 0.061578911663389135 11109 52-53 8.719125446319254E-4 11109 54-55 0.38475316492669265 11109 56-57 0.36169423524592403 11109 58-59 0.550450534073402 11109 60-61 -0.19520212860985353 11109 62-63 -0.49781218423044393 11109 64-65 -0.43636584632687203 11109 66-67 0.6477656640899028 11109 68-69 0.4316869825984888 11109 70-71 0.34161710616184493 11109 72-73 -0.4285522823037269 11109 74-75 -0.4338938316692591 11109 76 0.7783730103075115 11110 1 -0.7270478126298272 11110 2 -0.7768668671405479 11110 3 -0.7775973116241062 11110 4 -0.010775023860329469 11110 5 -1.2426253874573732 11110 6 -0.8168196821544669 11110 7 -0.25410871282714 11110 8 -0.7415938038681986 11110 9 -0.3692968714335123 11110 10-11 -0.7109636019976051 11110 12-13 -1.314291364327083 11110 14-15 -1.5391379154093308 11110 16-17 -0.3842393109725819 11110 18-19 -0.5767739565651198 11110 20-21 -0.6851029722726381 11110 22-23 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-0.029651397444325767 21610 32-33 0.8598465924264076 21610 34-35 0.011652294962530618 21610 36-37 -0.13672055287189977 21610 38-39 0.1471425767489123 21610 40-41 0.21502814409032567 21610 42-43 -0.0685297616478735 21610 44-45 0.08466440332917458 21610 46-47 -0.2731564942674751 21610 48-49 -0.20684178809604958 21610 50-51 -0.34782613641353066 21610 52-53 0.049249316390785225 21610 54-55 -0.01909298891946065 21610 56-57 -0.033287735907922666 21610 58-59 0.3435695244580188 21610 60-61 -0.19384996514831343 21610 62-63 -0.30655617461505713 21610 64-65 0.20320747098082137 21610 66-67 -0.07684371091009723 21610 68-69 -0.6781387385553579 21610 70-71 -0.3119285669150784 21610 72-73 -0.14504867653449338 21610 74-75 0.5446217933307409 21610 76 0.3519907987690516 21611 1 -0.01253733294300119 21611 2 -0.27897404277597815 21611 3 0.15888773211149498 21611 4 -1.0251787399816266 21611 5 -0.05902951564857162 21611 6 -0.004142913531715919 21611 7 0.32556125664222435 21611 8 -0.2763106931150503 21611 9 -0.1876676968844464 21611 10-11 0.23952245318230325 21611 12-13 0.16960981381822648 21611 14-15 -0.12482035935845914 21611 16-17 0.5696837817993341 21611 18-19 0.09085420653963894 21611 20-21 0.18258512827241447 21611 22-23 0.1793334859211413 21611 24-25 0.22672480888390112 21611 26-27 -0.09530399542259715 21611 28-29 0.04324954648092216 21611 30-31 -0.44194643071428885 21611 32-33 0.07801733862412163 21611 34-35 0.34011890617925644 21611 36-37 0.2947269875273335 21611 38-39 -0.025861225532459287 21611 40-41 -0.3075256225446559 21611 42-43 -0.3622114394045255 21611 44-45 0.6124760121694841 21611 46-47 0.32166289736750286 21611 48-49 -0.5629269235523253 21611 50-51 0.07707138022145443 21611 52-53 0.5017950625884993 21611 54-55 -0.19649317903353136 21611 56-57 0.20791206209588253 21611 58-59 -0.1590222649906501 21611 60-61 0.3690436303840059 21611 62-63 -0.3642661912500387 21611 64-65 0.09838481176029035 21611 66-67 0.04427231808229948 21611 68-69 -0.4922948103234184 21611 70-71 -0.5655377636831389 21611 72-73 -0.34191327967137397 21611 74-75 0.09379152954747383 21611 76 -0.25184415845528463 21612 1 0.46322312333076354 21612 2 0.5693654296574806 21612 3 0.06717254675027817 21612 4 -0.5265154795253544 21612 5 0.4459906839711998 21612 6 0.7990593203085865 21612 7 0.15162173668024792 21612 8 0.32706383159978003 21612 9 -1.1541452396601102 21612 10-11 0.7667919398743166 21612 12-13 -0.6528266167901364 21612 14-15 0.004167580280324046 21612 16-17 0.4567589956776672 21612 18-19 0.355001366710745 21612 20-21 -0.02105971488347791 21612 22-23 0.3182207245142976 21612 24-25 0.1961654576481635 21612 26-27 0.5944090511553526 21612 28-29 0.1901423696748381 21612 30-31 0.8062861025556742 21612 32-33 0.4555497174264076 21612 34-35 0.3807929199625306 21612 36-37 1.0273419471281002 21612 38-39 0.5143300767489123 21612 40-41 0.6603406440903257 21612 42-43 1.5818608633521265 21612 44-45 0.5866175283291746 21612 46-47 -0.2594846192674751 21612 48-49 0.9396425869039504 21612 50-51 0.8455332385864693 21612 52-53 0.9301086913907852 21612 54-55 0.9359851360805393 21612 56-57 1.7264778890920773 21612 58-59 0.2283351494580188 21612 60-61 0.06982190985168657 21612 62-63 0.6055532003849429 21612 64-65 0.6778168459808214 21612 66-67 0.02862503908990277 21612 68-69 -0.2152481135553579 21612 70-71 -0.2806785669150784 21612 72-73 0.2944044484655066 21612 74-75 0.4274342933307409 21612 76 0.3871470487690516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 17.0 12 70.0 13 143.0 14 242.0 15 387.0 16 566.0 17 816.0 18 1175.0 19 1665.0 20 2461.0 21 3481.0 22 4859.0 23 6297.0 24 7653.0 25 8965.0 26 9928.0 27 11188.0 28 12540.0 29 14329.0 30 16904.0 31 20969.0 32 26656.0 33 37885.0 34 55341.0 35 64298.0 36 23847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.078007959505115 18.27770496928016 8.632516141443531 26.011770929771195 2 27.435727103579083 21.055779826441388 27.95604223840713 23.552450831572397 3 25.688641473590557 22.10115304613387 25.370922557141313 26.839282923134267 4 22.009474456240756 16.645224898101503 28.461543085931396 32.88375755972635 5 22.09153430883361 30.982854600762284 30.05374469466521 16.8718663957389 6 38.315939450048695 30.673251493910136 14.936396099602026 16.074412956439144 7 35.874221096011475 26.83818335392375 16.757294569239416 20.530300980825363 8 32.787570186723734 29.938620432602708 16.893809140205118 20.380000240468434 9 29.99513051424174 16.414675788435872 16.438722631686524 37.15147106563586 10-11 33.385785825503035 22.64985782218455 21.817832043813613 22.146524308498808 12-13 32.0719242162596 21.9313551769174 21.107899850759054 24.888820756063947 14-15 30.588185785910955 21.25650767695471 19.317580647100552 28.837725890033788 16-17 30.839775883420906 24.354793137030935 20.10346154308593 24.701969436462228 18-19 30.33142661332259 24.753594262405954 20.903232206033973 24.01174691823748 20-21 30.41479364020157 24.13765636107592 20.944531363893912 24.5030186348286 22-23 31.088894332867923 23.664075098855815 20.71636084362972 24.530669724646543 24-25 30.841371515827383 23.719426601299133 20.5578583817027 24.881343501170782 26-27 30.45176571726581 23.18419759320796 21.71388121142167 24.650155478104566 28-29 30.662881292758293 23.551778865229466 22.13451804114415 23.65082180086809 30-31 31.17497561496137 23.268061085085375 21.342056338832556 24.214906961120704 32-33 30.374515117221023 24.06386249994364 21.54705598564401 24.014566397191324 34-35 30.98228939347485 23.327682291197 21.825346727505547 23.864681587822606 36-37 31.23763834532677 22.86252337066628 21.80986647910016 24.08997180490679 38-39 30.314247625746393 23.274974562910394 22.709872897213117 23.700904914130096 40-41 30.669540268753938 22.206691945948627 22.909612289157714 24.214155496139725 42-43 29.969881328828556 22.349436702696853 23.091582402520107 24.589099565954477 44-45 29.395851305897807 22.719976674491935 23.550647312907483 24.33352470670278 46-47 29.260631382774864 22.259511696587587 23.66009633810014 24.819760582537402 48-49 28.5649445119092 21.994896057520048 24.68603840280867 24.75412102776208 50-51 28.083556768585204 22.52963773430643 24.488553702612688 24.89825179449568 52-53 27.890280265958083 23.470620769258517 24.092682545598826 24.54641641918457 54-55 27.752010917266833 23.1359187697635 23.89715165141696 25.214918661552705 56-57 27.374024599920645 23.478435993314978 23.64556155390701 25.501977852857365 58-59 27.6084813216145 23.105860215700183 23.992136682257037 25.293521780428275 60-61 27.842336872227097 23.2483377619603 23.564102872395427 25.345222493417175 62-63 27.788732648386727 23.2183973692279 23.56753377139456 25.425336210990807 64-65 28.59414428428221 22.870385817150535 22.760221050938807 25.77524884762845 66-67 28.466631097217316 22.77907614617541 22.15280334853802 26.601489408069256 68-69 28.48818772196397 22.644796029860213 22.30182741254075 26.565188835635073 70-71 29.481624426820087 22.35322761723981 22.1961714362149 25.968976519725206 72-73 29.99002056005098 22.257758112803742 21.409205131596348 26.34301619554893 74-75 30.35467644172314 22.6338246411379 21.402925002291965 25.608573914846993 76 31.129032742881364 21.99150542708825 21.36117565370037 25.51828617633002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 1.0 7 1.0 8 1.0 9 2.0 10 3.5 11 8.0 12 12.0 13 18.5 14 28.5 15 32.5 16 42.0 17 51.0 18 67.5 19 118.0 20 171.5 21 191.0 22 237.5 23 392.5 24 614.0 25 727.0 26 869.5 27 1082.0 28 1518.0 29 1884.0 30 2143.5 31 2729.5 32 3640.5 33 4225.0 34 4720.5 35 5936.0 36 7418.5 37 8181.0 38 8909.5 39 10637.5 40 12422.0 41 13565.5 42 13924.0 43 14467.5 44 15362.5 45 15869.0 46 16024.0 47 15380.5 48 14224.5 49 13156.0 50 12600.0 51 11930.0 52 10417.5 53 9036.0 54 8497.0 55 7988.5 56 7181.0 57 6521.5 58 6161.0 59 6026.0 60 5863.0 61 5813.0 62 5791.0 63 5885.5 64 5839.0 65 5652.5 66 5662.0 67 5717.0 68 5664.5 69 5462.5 70 5287.5 71 5262.0 72 5173.5 73 4937.5 74 4708.5 75 4627.0 76 4348.5 77 3803.5 78 3191.5 79 2846.0 80 2565.0 81 2031.0 82 1529.5 83 1281.0 84 1081.5 85 748.0 86 533.5 87 453.0 88 407.0 89 339.5 90 315.5 91 300.5 92 288.0 93 300.0 94 361.0 95 498.0 96 586.0 97 709.5 98 1169.0 99 9181.5 100 16858.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.005855406331534E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.001502927703165767 8 0.0 9 0.0 10-11 6.011710812663068E-4 12-13 1.502927703165767E-4 14-15 0.0 16-17 0.0 18-19 3.005855406331534E-4 20-21 1.502927703165767E-4 22-23 7.514638515828835E-4 24-25 3.005855406331534E-4 26-27 1.502927703165767E-4 28-29 0.0 30-31 1.502927703165767E-4 32-33 1.502927703165767E-4 34-35 6.011710812663068E-4 36-37 6.011710812663068E-4 38-39 1.502927703165767E-4 40-41 1.502927703165767E-4 42-43 0.0 44-45 3.005855406331534E-4 46-47 4.508783109497301E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0018035132437989204 64-65 1.502927703165767E-4 66-67 4.508783109497301E-4 68-69 1.502927703165767E-4 70-71 1.502927703165767E-4 72-73 0.0 74-75 1.502927703165767E-4 76 3.005855406331534E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 332684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.31128535131273 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59898472302787 44.47297556433607 2 9.606144563584486 11.01080984419104 3 5.292971614540743 9.10041019672029 4 2.8807344485721385 6.603943760138975 5 1.5034943076838174 4.308359564587081 6 0.8287002154372008 2.849632471056945 7 0.5166138849522187 2.0725464043862796 8 0.3234705055968298 1.4830808359194652 9 0.23633321675218963 1.2190104380950544 >10 1.1967344404482625 11.409987848616554 >50 0.014236271463854654 0.4828913436642777 >100 0.0010545386269521784 0.10999527477709282 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 5.272693134760892E-4 4.8763564535108435 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16137 4.850548869197196 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.038474949201043634 0.0 2 0.0 0.0 0.0 0.04148080460737517 0.0 3 0.0 0.0 0.0 0.042382561229274626 0.0 4 0.0 0.0 0.0 0.05170071298890238 0.0 5 0.0 0.0 0.0 0.05170071298890238 0.0 6 0.0 0.0 0.0 0.05380481177333445 0.0 7 0.0 0.0 0.0 0.05590891055776653 0.0 8 0.0 0.0 0.0 0.05590891055776653 0.0 9 0.0 0.0 0.0 0.05771242380156545 0.0 10 0.0 0.0 0.0 0.05951593704536437 0.0 11 0.0 0.0 0.0 0.06041769366726383 0.0 12 0.0 0.0 0.0 0.06041769366726383 0.0 13 0.0 0.0 0.0 0.06162003582979644 0.0 14 0.0 0.0 0.0 0.06552764785802744 0.0 15 0.0 0.0 0.0 0.06612881893929375 0.0 16 0.0 0.0 0.0 0.06883408880499213 0.0 17 0.0 0.0 0.0 0.06913467434562527 0.0 18 0.0 0.0 0.0 0.07003643096752474 0.0 19 0.0 0.0 0.0 0.07334287191448942 0.0 20 0.0 0.0 0.0 0.07424462853638888 0.0 21 0.0 0.0 0.0 0.0748457996176552 0.0 22 0.0 0.0 0.0 0.08506570799918241 0.0 23 0.0 0.0 0.0 0.08506570799918241 0.0 24 0.0 0.0 0.0 0.08867273448678024 0.0 25 0.0 0.0 0.0 0.09077683327121232 0.0 26 0.0 0.0 0.0 0.09318151759627755 0.0 27 0.0 0.0 0.0 0.1238412427408592 0.0 28 0.0 0.0 0.0 0.16201560640126966 0.0 29 0.0 0.0 0.0 0.20319582546801168 0.0 30 0.0 0.0 0.0 0.2545959529162809 0.0 31 0.0 0.0 0.0 0.31561481766481103 0.0 32 0.0 0.0 0.0 0.38474949201043634 0.0 33 0.0 0.0 0.0 0.4824397927162112 0.0 34 0.0 0.0 0.0 0.6441548135768477 0.0 35 0.0 0.0 0.0 0.8419400993134626 0.0 36 0.0 0.0 0.0 1.1488379362999122 0.0 37 0.0 0.0 0.0 1.6231619194190283 0.0 38 0.0 0.0 0.0 2.0800519411814213 0.0 39 0.0 0.0 0.0 2.643349244327951 0.0 40 0.0 0.0 0.0 3.3283836914309073 0.0 41 0.0 0.0 0.0 4.083153983960756 0.0 42 0.0 0.0 0.0 5.038114246552284 0.0 43 0.0 0.0 0.0 5.9603106852147985 0.0 44 0.0 0.0 0.0 6.872587801036419 0.0 45 0.0 0.0 0.0 7.70009979439949 0.0 46 0.0 0.0 0.0 8.640331365499994 0.0 47 0.0 0.0 0.0 9.51683880198627 0.0 48 0.0 0.0 0.0 10.286337786007142 0.0 49 0.0 0.0 0.0 11.006240155823544 0.0 50 0.0 0.0 0.0 11.697286313739164 0.0 51 0.0 0.0 0.0 12.444842553293816 0.0 52 0.0 0.0 0.0 13.296100804366906 0.0 53 0.0 0.0 0.0 14.240540573036276 0.0 54 0.0 0.0 0.0 15.114042154116218 0.0 55 0.0 0.0 0.0 15.866708347861634 0.0 56 0.0 0.0 0.0 16.577893736999677 0.0 57 0.0 0.0 0.0 17.27404984910606 0.0 58 0.0 0.0 0.0 17.92601988673937 0.0 59 0.0 0.0 0.0 18.535907948684038 0.0 60 0.0 0.0 0.0 19.10251169277753 0.0 61 0.0 0.0 0.0 19.717509708912964 0.0 62 0.0 0.0 0.0 20.331605968426494 0.0 63 0.0 0.0 0.0 21.06232941770569 0.0 64 0.0 0.0 0.0 21.813793269288574 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATAGAT 35 0.0012544831 40.0 32 TTAGAGT 60 1.2139542E-5 35.0 4 AGTGTCC 40 0.002416971 35.0 20 CGGAAAT 45 0.004304104 31.111113 55 GCGAATA 45 0.004304104 31.111113 57 TGTGCGC 45 0.004304104 31.111113 3 CGAATAG 45 0.004304104 31.111113 58 TTACGTC 45 0.004304104 31.111113 38 CGCAATA 45 0.004304104 31.111113 57 GCCGTAT 45 0.004304104 31.111113 54 GATAGCG 45 0.004304104 31.111113 46 GTTGCAC 45 0.004304104 31.111113 39 TCGGAAA 50 0.0072031203 28.000002 54 GCGAAAT 65 7.9473876E-4 26.923077 11 CATGTAA 80 8.6525084E-5 26.25 33 CAGCGAA 70 0.0012247228 25.0 25 TTTCCCA 70 0.0012247228 25.0 29 GTACTGA 70 0.0012247228 25.0 1 GTAGATC 100 1.3948806E-5 24.500002 38 ACAGTTA 75 0.0018303066 23.333334 51 >>END_MODULE