FastQCFastQC Report
Wed 25 May 2016
SRR1779680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332684
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT95822.880210650346876No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG12790.3844489064698032No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC6340.19057123276141924No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAGT150.002219403270.0326448
ACGTATG150.002220727570.022140
AGGGGGG11550.053.62911270
GACGTAT200.006934919452.52447546
TATCCGT200.006951435552.49289735
CGTATGC17450.051.7641941
TATGCCG16850.051.737443
TCGTATG18100.051.4527140
CTCGTAT17950.051.28981439
GTATGCC17150.051.0365242
TCTCGTA18400.050.98669438
ATCTCGT18250.050.81504437
CCGTCTT16550.050.7789547
ATGCCGT16800.050.4325944
CTTGAAA18000.050.3618757
GTAGATC18550.050.18189233
CTGCTTG17550.050.06525854
GTCTTCT17250.049.93631449
GCCGTCT17050.049.7005846
TAGATCT18550.049.42727734
Produced by FastQC (version 0.11.5)